Influence of the perivitelline space on the quantification of internal concentrations of chemicals in eggs of zebrafish embryos (Danio rerio)

Brox, Stephan; Ritter, Axel P.; Küster, Eberhard; Reemtsma, Thorsten

doi:10.1016/j.aquatox.2014.10.008

Cited by 30 publications

(27 citation statements)

References 29 publications

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“…Such similar patterns of diphenhydramine uptake for dechorionated and chorionated 0 to 4 dpf embryos are interesting because it has been postulated that the chorion could act as a physical barrier to bioaccumulation or function to contain the osmoregulatory fluid (perivitelline fluid) that could interact with xenobiotics and potentially alter toxicity response Developmental stage-dependent bioconcentration by zebrafish-Environmental Toxicology and Chemistry, 2018;37:1175-1181 thresholds [30]. The chorion is an acellular membrane that envelopes teleost fish embryos [31] and is composed largely of proteins and glycoproteins that structurally and functionally differ between species.…”

Section: Resultsmentioning

confidence: 92%

Differential uptake of and sensitivity to diphenhydramine in embryonic and larval zebrafish

Kristofco

Haddad

Chambliss³

et al. 2018

Enviro Toxic and Chemistry

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The zebrafish fish embryo toxicity (FET) test is increasingly employed for alternative toxicity studies, yet our previous research identified increased sensitivity of zebrafish slightly older than embryos employed in FET methods (0-4 d postfertilization [dpf]). We identified rapid steady-state accumulation of diphenhydramine across zebrafish embryo and larval stages. However, significantly (p < 0.05) lower accumulation was observed at 48 h compared to 96 h in chorionated and dechorionated embryos (0-4 dpf), but not in zebrafish at 7 to 11 and 14 to 18 dpf. Increased uptake and toxicity of diphenhydramine was further observed in zebrafish at 7 to 11 and 14 to 18 dpf compared with 0-4 dpf embryos with chorion or dechorionated, which indicates that differential zebrafish sensitivity with age is associated with accumulation resulting from gill and other toxicokinetic and toxicodynamic changes during development. Environ Toxicol Chem 2018;37:1175-1181. © 2017 SETAC.

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Section: Resultsmentioning

confidence: 92%

Differential uptake of and sensitivity to diphenhydramine in embryonic and larval zebrafish

Kristofco

Haddad

Chambliss³

et al. 2018

Enviro Toxic and Chemistry

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“…The zebrafish chorion has been reported to reduce the permeability of organic molecules (Brox et al, 2014) ; however, for heavy metals it could also represent a barrier since these could accumulate at the surface or inside the chorion (Henn and Braunbeck, 2011). Moreover, in the case of arsenic, low bioaccumulation factors have been reported for As 2 O 3 in zebrafish larvae, which may also affect its bioavailability (López-Serrano Oliver et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

Arsenic (III, V), indium (III), and gallium (III) toxicity to zebrafish embryos using a high-throughput multi-endpoint in vivo developmental and behavioral assay

et al. 2016

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Gallium arsenide (GaAs), indium gallium arsenide (InGaAs) and other III/V materials are finding increasing application in microelectronic components. The rising demand for III/V-based products is leading to increasing generation of effluents containing ionic species of gallium, indium, and arsenic. The ecotoxicological hazard potential of these streams is unknown. While the toxicology of arsenic is comprehensive, much less is known about the effects of In(III) and Ga(III). The embryonic zebrafish was evaluated for mortality, developmental abnormalities, and photomotor response (PMR) behavior changes associated with exposure to As(III), As(V), Ga(III), and In(III). The As(III) lowest observable effect level (LOEL) for mortality was 500 μM at 24 and 120 hours post-fertilization (hpf). As(V) exposure was associated with significant mortality at 63 μM. The Ga(III)-citrate LOEL was 113 μM at 24 and 120 hpf. There was no association of significant mortality over the tested range of In(III)-citrate (56-900 μM) or sodium citrate (213-3400 μM) exposures. Only As(V) resulted in significant developmental abnormalities with LOEL of 500 μM. Removal of the chorion prior to As(III) and As(V) exposure was associated with increased incidence of mortality and developmental abnormality suggesting that the chorion may normally attenuate mass uptake of these metals by the embryo. Finally, As(III), As(V), and In(III) caused PMR hypoactivity (49-69% of control PMR) at 900-1,000 μM. Overall, our results represent the first characterization of multidimensional toxicity effects of III/V ions in zebrafish embryos helping to fill a significant knowledge gap, particularly in Ga(III) and In(III) toxicology.

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“…As Acyclovir and Zidovudine have low logP values, this can be expected as the embryo is a lipid structure. However, it is indicated here that for compounds with lower logP value and thus more hydrophilic, the PVS might influence exposure to the embryo in the presence of a chorion (Brox, ). For Metoprolol nearly all of the compound was found within the embryo.…”

Section: Discussionmentioning

confidence: 96%

Visualizing Compound Distribution during Zebrafish Embryo Development: The Effects of Lipophilicity and DMSO

Koning¹,

Beekhuijzen²,

Tobor-Kaplon³

et al. 2015

Birth Defects Research Pt B

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The predictability of the zebrafish embryo model is highly influenced by internal exposure of the embryo/larva. As compound uptake is likely to be influenced by factors such as lipophilicity, solvent use, and chorion presence, this article focuses on investigating their effects on compound distribution within the zebrafish embryo. To visualize compound uptake and distribution, zebrafish embryos were exposed for 96 hr, starting at 4 hr postfertilization, to water-soluble dyes: Schiff's reagent (logP -4.63), Giemsa stain (logP -0.77), Van Gierson stain (logP 1.64), Cresyl fast violet (logP 3.5), Eosine Y (logP 4.8), Sudan III (logP 7.5), and Oil red O (logP 9.81), with and without 1% dimethyl-sulfoxide (DMSO). Three additional compounds were used to analytically determine the uptake and distribution: Acyclovir (logP -1.56), Zidovudine (logP 0.05), and Metoprolol Tartrate Salt (logP 1.8). Examinations were performed every 24 hr. Both methods (visualization and specific analysis) showed that exposure to higher logP values results in higher compound uptake. Specific analysis showed that for lipophilic compounds >90% of compound is taken up by the embryo. For hydrophilic compounds, >90% of compound within the complete egg could not be associated to embryo or chorion and is probably distributed into the perivitelline space. Overall, internal exposure analyses on at least two occasions (i.e., before and after hatching) is crucial for interpretation of zebrafish embryotoxicity data, especially for compounds with extreme logP values. DMSO did not affect exposure when examined with the visualization method, however, this method might be not sensitive enough to draw hard conclusions.

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Influence of the perivitelline space on the quantification of internal concentrations of chemicals in eggs of zebrafish embryos (Danio rerio)

Cited by 30 publications

References 29 publications

Differential uptake of and sensitivity to diphenhydramine in embryonic and larval zebrafish

Differential uptake of and sensitivity to diphenhydramine in embryonic and larval zebrafish

Arsenic (III, V), indium (III), and gallium (III) toxicity to zebrafish embryos using a high-throughput multi-endpoint in vivo developmental and behavioral assay

Visualizing Compound Distribution during Zebrafish Embryo Development: The Effects of Lipophilicity and DMSO

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