Zebrafish (Danio rerio) embryos as a model for testing proteratogens

Weigt, Stefan; Huebler, Nicole; Strecker, Ruben; Braunbeck, Thomas; Broschard, Thomas H.

doi:10.1016/j.tox.2011.01.004

Cited by 185 publications

(120 citation statements)

References 112 publications

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“…For example, Hallare and colleagues [12] recorded an up-regulation of Hsp70 levels in zebrafish (Danio rerio) embryos exposed to contaminated sediments and organic extracts collected along the Laguna Lake, Philippines. Their study also confirmed that developing zebrafish embryos are able to detect chemical stressors in their immediate environment [13][14][15][16].…”

Section: Introductionmentioning

confidence: 57%

Embryotoxic and proteotoxic effects of water and sediment from the Neckar River (Southern Germany) to zebrafish (Danio rerio) embryos

et al. 2014

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Background: The Neckar River (Southern Germany) represents an aquatic system strongly affected by numerous anthropogenic activities. Thus, it is an excellent model for ecotoxicological investigations. The present study aims to assess time and spatial variations of embryo-and proteotoxic effects in surface water and sediment. For this end, embryos of zebrafish (Danio rerio) were exposed to Neckar River samples collected in the Tübingen region in different seasons over 2 years. Additionally, quantification of the heat shock (stress) protein Hsp70 was carried out in newly hatched larvae; furthermore, physico-chemical water parameters were measured in order to gain baseline information about the limnologic conditions.

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Section: Introductionmentioning

confidence: 57%

Embryotoxic and proteotoxic effects of water and sediment from the Neckar River (Southern Germany) to zebrafish (Danio rerio) embryos

et al. 2014

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“…The average mortality of the control (the 17 chamber) was 8. of C1, C24, C23, C22, C21, C20, C19, and C18 in turn) or Cu 2þ (concentration from high to low of C9, C10, C11, C12, C13, C14, C15, and C16 in turn) solution, but there is no significant difference at the low concentration (Pb 2þ , 18,19,20;Cu,14,15,16). The mortality of mixture solution (Pb 2þ and Cu 2þ (C2-C8)) was obviously higher than the control group ( Fig.…”

Section: Embryo Culturing On Chipsmentioning

confidence: 98%

“…18 The normal and healthy developed eggs at different developmental stages were then selected by using a sterile micropipette under a stereomicroscope (SMZ-T4, Optec, Chongqing, China) at 3 hpf (Ref. 19) and cleaned twice with ultrapure water 20 for the subsequent microfluidic application. Ultrapure water medium was aerated for at least half an hour before addition of the test chemicals.…”

mentioning

confidence: 99%

Comparative toxicity of lead (Pb2+), copper (Cu2+), and mixtures of lead and copper to zebrafish embryos on a microfluidic chip

Yang

Chen

et al. 2015

Biomicrofluidics

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Investigations were conducted to determine acute effects of Pb 2þ and Cu 2þ presented individually and collectively on zebrafish embryos. Aquatic safety testing requires a cheap, fast, and highly efficient platform for real-time evaluation of single and mixture of metal toxicity. In this study, we have developed a microfluidic system for phenotype-based evaluation of toxic effects of Pb 2þ and Cu 2þ using zebrafish (Danio rerio) embryos. The microfluidic chip is composed of a disc-shaped concentration gradient generator and 24 culture chambers, which can generate one blank solution, seven mixture concentrations, and eight single concentrations for each metal solution, thus enabling the assessment of zebrafish embryos.To test the accuracy of this new chip platform, we have examined the toxicity and teratogenicity of Pb 2þ and Cu 2þ on embryos. The individual and combined impact of Pb 2þ and Cu 2þ on zebrafish embryonic development was quantitatively assessed by recording a series of physiological indicators, such as spontaneous motion at 22 hours post fertilization (hpf), mortality at 24 hpf, heartbeat and body length at 96 hpf, etc. It was found that Pb 2þ or Cu 2þ could induce deformity and cardiovascular toxicity in zebrafish embryos and the mixture could induce more severe toxicity. This chip is a multiplexed testing apparatus that allows for the examination of toxicity and teratogenicity for substances and it also can be used as a potentially costeffective and rapid aquatic safety assessment tool. V C 2015 AIP Publishing LLC.

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“…For this reason, an early focus in alternative method development was on tests for embryonic malformations (Augustine-Rauch et al, 2010). The most complete reflection of embryonic development apparently can be achieved with zebrafish embryos (Selderslaghs et al, 2011;Sukardi et al, 2011;Weigt et al, 2010Weigt et al, , 2011Yang et al, 2009), for example using dynamic cell imaging, or frog eggs (FETAX assay) (Hoke and Ankley, 2005), which has been evaluated more critically by IC-CVAM 1 . It seems to be timely to evaluate available protocols and datasets and define a protocol for formal validation.…”

Section: Reduction To Key Eventsmentioning

confidence: 99%

A roadmap for the development of alternative (non-animal) methods for systemic toxicity testing

Basketter

Clewell

Kimber

et al. 2012

ALTEX

203

165

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Zebrafish (Danio rerio) embryos as a model for testing proteratogens

Cited by 185 publications

References 112 publications

Embryotoxic and proteotoxic effects of water and sediment from the Neckar River (Southern Germany) to zebrafish (Danio rerio) embryos

Embryotoxic and proteotoxic effects of water and sediment from the Neckar River (Southern Germany) to zebrafish (Danio rerio) embryos

Comparative toxicity of lead (Pb2+), copper (Cu2+), and mixtures of lead and copper to zebrafish embryos on a microfluidic chip

A roadmap for the development of alternative (non-animal) methods for systemic toxicity testing

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