Zebrafish as an alternative method for determining the embryo toxicity of plant products: a systematic review

Falcão, Maria Alice Pimentel; Dolabella, Silvio Santana; Guimarães, Adriana Gibara; Walker, Cristiani Isabel Banderó

doi:10.1007/s11356-018-3399-7

Cited by 27 publications

(15 citation statements)

References 83 publications

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“…The embryonic zebrafish (Danio rerio) is a useful tool for rapid chemical bioactivity screening, capable of producing concentration-response data for hundreds of chemicals in a matter of days [4][5][6][7][8]. Zebrafish embryos develop rapidly and transparently, allowing for visual observation of tissue development in real time.…”

Section: Introductionmentioning

confidence: 99%

Systematic Assessment of Exposure Variations on Observed Bioactivity in Zebrafish Chemical Screening

Wilson

Truong

Simonich

et al. 2020

Toxics

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The embryonic zebrafish is a powerful tool for high-throughput screening of chemicals. While this model has significant potential for use in safety assessments and chemical prioritization, a lack of exposure protocol harmonized across laboratories has limited full model adoption. To assess the potential that exposure protocols alter chemical bioactivity, we screened a set of eight chemicals and one 2D nanomaterial across four different regimens: (1) the current Tanguay laboratory’s standard protocol of dechorionated embryos and static exposure in darkness; (2) exposure with chorion intact; (3) exposure under a 14 h light: 10 h dark cycle; and (4) exposure with daily chemical renewal. The latter three regimens altered the concentrations, resulting in bioactivity of the test agents compared to that observed with the Tanguay laboratory’s standard regimen, though not directionally the same for each chemical. The results of this study indicate that with the exception for the 2D nanomaterial, the screening design did not change the conclusion regarding chemical bioactivity, just the nominal concentrations producing the observed activity. Since the goal of tier one chemical screening often is to differentiate active from non-active chemicals, researchers could consider the trade-offs regarding cost, labor, and sensitivity in their study design without altering hit rates. Taken further, these results suggest that it is reasonably feasible to reach agreement on a standardized exposure regiment, which will promote data sharing without sacrificing data content.

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Section: Introductionmentioning

confidence: 99%

Systematic Assessment of Exposure Variations on Observed Bioactivity in Zebrafish Chemical Screening

Wilson

Truong

Simonich

et al. 2020

Toxics

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show abstract

“…Zebrafish are used in the biotechnological field as a screening test because they possess many advantages, such as low cost, short generation time, genetic similarity, large number of eggs, and transparent embryos, to evaluate biological activity. Furthermore, the initial entry step of the in vivo study can easily measure heart rate and survival rate in zebrafish as a toxicity indicator of tested samples [ 38 ]. Furthermore, the heart rate and survival rate of zebrafish treated with tested samples can be used as indicators of toxicity [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Potential Antioxidant Properties of Enzymatic Hydrolysates from Stichopus japonicus against Hydrogen Peroxide-Induced Oxidative Stress

Lee

Kim

et al. 2021

Antioxidants

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A comprehensive antioxidant evaluation was performed on enzymatic hydrolysates of Stichopusjaponicus (S. japonicus) using Vero cells and zebrafish models for in vitro and in vivo studies, respectively. S. japonicus was hydrolyzed with food-grade enzymes (alcalase, α-chymotrypsin, flavourzyme, kojizyme, neutrase, papain, pepsin, protamex, and trypsin), and the free radical scavenging activities were screened via electron spin resonance (ESR) spectroscopy. According to the results, the enzymatic hydrolysates contained high protein and relatively low polysaccharide and sulfate contents. Among these hydrolysates, the α-chymotrypsin assisted hydrolysate from S. japonicus (α-chy) showed high yield and protein content, and strong hydroxyl radical scavenging activity. Therefore, α-chy was chosen for further purification. The α-chy was fractionated by ultrafiltration into three ultrafiltration (UF) fractions based on their molecular weight: >10 kDa (α-chy-I), 5–10 kDa (α-chy-II), and <5 kDa (α-chy-III), and we evaluated their antioxidant properties in H2O2 exposed Vero cells. The α-chy and its UF fractions significantly decreased the intracellular reactive oxygen species (ROS) generation and increased cell viability in H2O2 exposed Vero cells. Among them, α-chy-III effectively declined the intracellular ROS levels and increased cell viability and exhibited protection against H2O2 induced apoptotic damage. Furthermore, α-chy-III remarkably attenuated the cell death, intracellular ROS and lipid peroxidation in H2O2 exposed zebrafish embryos. Altogether, our findings demonstrated that α-chy and its α-chy-III from S. japonicus possess strong antioxidant activities that could be utilized as a bioactive ingredient for functional food industries.

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“…Belonging to the Cannabaceae family, Cannabis sativa is a plant with more than 500 active chemical compounds already isolated, among which about 17% of these are classified as cannabinoids. These are widely used in different preparations and formulations for both medical and recreational purposes as they can decrease the level of stress, anxiety, and depression [ 1 – 3 ]. Studies in animal models have shown that cannabidiol (CBD) can be used to treat a variety of diseases such as autism, fibromyalgia, multiple sclerosis, Alzheimer's, Parkinson's, epilepsy and seizures, schizophrenia, and psychosis [ 4 – 12 ], among others.…”

Section: Introductionmentioning

confidence: 99%

The endocannabinoid system in zebrafish and its potential to study the effects of Cannabis in humans

et al. 2022

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Zebrafish is considered an unprecedented animal model in drug discovery. A review of the literature presents highlights and elucidates the biological effects of chemical components found in Cannabis sativa. Particular attention is paid to endocannabinoid system (eCB) and its main receptors (CB1 and CB2). The zebrafish model is a promising one for the study of cannabinoids because of the many similarities to the human system. Despite the recent advances on the eCB system, there is still the need to elucidate some of the interactions and, thus, the zebrafish model can be used for that purpose as it respects the 3Rs concept and reduced time and costs. In view of the relevance of cannabinoids in the treatment and prevention of diseases, as well as the importance of the zebrafish animal model in elucidating the biological effects of new drugs, the aim of this study was to bring to light information on the use of the zebrafish animal model in testing C. sativa-based medicines.

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Zebrafish as an alternative method for determining the embryo toxicity of plant products: a systematic review

Cited by 27 publications

References 83 publications

Systematic Assessment of Exposure Variations on Observed Bioactivity in Zebrafish Chemical Screening

Systematic Assessment of Exposure Variations on Observed Bioactivity in Zebrafish Chemical Screening

Potential Antioxidant Properties of Enzymatic Hydrolysates from Stichopus japonicus against Hydrogen Peroxide-Induced Oxidative Stress

The endocannabinoid system in zebrafish and its potential to study the effects of Cannabis in humans

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