Zebrafish: A promising in vivo model for assessing the delivery of natural products, fluorescence dyes and drugs across the blood-brain barrier

Li, Ye; Chen, Tongkai; Miao, Xiaoqing; Xiang, Yi; Wang, Xueqing; Zhao, Haitao; Lee, Simon Ming‐Yuen; Zheng, Ying

doi:10.1016/j.phrs.2017.08.017

Cited by 58 publications

(39 citation statements)

References 96 publications

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“…In embryonic chick, aquaporin 4, a molecular water channels identified in mammalian BBB, was described in astroglia (Nico et al, 2001 ). In zebrafish, anatomical analysis and transmission electron microscopy revealed that adult endothelial cells display physical barrier properties and express Claudin-5 and ZO-1 proteins (Jeong et al, 2008 ; Eliceiri et al, 2011 ; Li et al, 2017 ). Complete maturation of the zebrafish BBB occurs between 3 and 10 days when expression of Claudin-5 and ZO-1 proteins are detected in cerebral endothelial vascular cells (Jeong et al, 2008 ; Eliceiri et al, 2011 ; Fleming et al, 2013 ; Li et al, 2017 ).…”

Section: Steroids and Lipoproteins: From Periphery To The Brainmentioning

confidence: 99%

“…In zebrafish, anatomical analysis and transmission electron microscopy revealed that adult endothelial cells display physical barrier properties and express Claudin-5 and ZO-1 proteins (Jeong et al, 2008 ; Eliceiri et al, 2011 ; Li et al, 2017 ). Complete maturation of the zebrafish BBB occurs between 3 and 10 days when expression of Claudin-5 and ZO-1 proteins are detected in cerebral endothelial vascular cells (Jeong et al, 2008 ; Eliceiri et al, 2011 ; Fleming et al, 2013 ; Li et al, 2017 ). Although some studies have highlighted the presence of stellate astrocytes in some fish (Kawai et al, 2001 ; Alunni et al, 2005 ; Strobl-Mazzulla et al, 2010 ), immunohistochemistry against GFAP or S100β protein has generally failed to demonstrate the presence of star-shaped cells resembling mammalian astrocytes in brain of many fish, including trout, carp, and zebrafish (Arochena et al, 2004 ; Pellegrini et al, 2005 ; Grupp et al, 2010 ; März et al, 2010 ).…”

Section: Steroids and Lipoproteins: From Periphery To The Brainmentioning

confidence: 99%

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Steroid Transport, Local Synthesis, and Signaling within the Brain: Roles in Neurogenesis, Neuroprotection, and Sexual Behaviors

et al. 2018

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Sex steroid hormones are synthesized from cholesterol and exert pleiotropic effects notably in the central nervous system. Pioneering studies from Baulieu and colleagues have suggested that steroids are also locally-synthesized in the brain. Such steroids, called neurosteroids, can rapidly modulate neuronal excitability and functions, brain plasticity, and behavior. Accumulating data obtained on a wide variety of species demonstrate that neurosteroidogenesis is an evolutionary conserved feature across fish, birds, and mammals. In this review, we will first document neurosteroidogenesis and steroid signaling for estrogens, progestagens, and androgens in the brain of teleost fish, birds, and mammals. We will next consider the effects of sex steroids in homeostatic and regenerative neurogenesis, in neuroprotection, and in sexual behaviors. In a last part, we will discuss the transport of steroids and lipoproteins from the periphery within the brain (and vice-versa) and document their effects on the blood-brain barrier (BBB) permeability and on neuroprotection. We will emphasize the potential interaction between lipoproteins and sex steroids, addressing the beneficial effects of steroids and lipoproteins, particularly HDL-cholesterol, against the breakdown of the BBB reported to occur during brain ischemic stroke. We will consequently highlight the potential anti-inflammatory, anti-oxidant, and neuroprotective properties of sex steroid and lipoproteins, these latest improving cholesterol and steroid ester transport within the brain after insults.

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Section: Steroids and Lipoproteins: From Periphery To The Brainmentioning

confidence: 99%

Section: Steroids and Lipoproteins: From Periphery To The Brainmentioning

confidence: 99%

Steroid Transport, Local Synthesis, and Signaling within the Brain: Roles in Neurogenesis, Neuroprotection, and Sexual Behaviors

et al. 2018

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“…Especially, permeability of the blood-brain-barrier (BBB) determines whether a cardiovascular drug candidate has a neurological impact even if the brain is not the therapeutic target. Interestingly, BBB in zebrafish has rather low permeability and includes strong tight junctions, drug transporters and protein expression compared to the BBB in higher vertebrates [33].…”

Section: Drug Absorption and Drug Metabolism In The Zebrafishmentioning

confidence: 99%

Streamlining drug discovery assays for cardiovascular disease using zebrafish

Pott

Rottbauer

Just

2019

Expert Opinion on Drug Discovery

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Introduction: In the last decade, our armamentarium of cardiovascular drug therapy has expanded significantly. Using innovative functional genomics strategies such as genome editing by CRISPR/Cas9 as well as high-throughput assays to identify bioactive small chemical compounds has significantly facilitated elaboration of the underlying pathomechanism in various cardiovascular diseases. However, despite scientific progress approvals for cardiovascular drugs has stagnated significantly compared to other fields of drug discovery and therapy during the past years. Areas covered: In this review, the authors discuss the aspects and pitfalls during the early phase of cardiovascular drug discovery and describe the advantages of zebrafish as an in vivo organism to model human cardiovascular diseases (CVD) as well as an in vivo platform for high-throughput chemical compound screening. They also highlight the emerging, promising techniques of automated read-out systems during high-throughput screening (HTS) for the evaluation of important cardiac functional parameters in zebrafish with the potential to streamline CVD drug discovery. Expert opinion: The successful identification of novel drugs to treat CVD is a major challenge in modern biomedical and clinical research. In this context, the definition of the etiologic fundamentals of human cardiovascular diseases is the prerequisite for an efficient and straightforward drug discovery.

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“…Complementing rodent findings, the zebrafish ( Danio rerio ) is rapidly emerging as an important model organism to study impulsive (Parker, Ife, et al, 2013) and aggressive behaviors (de Abreu, et al., 2019; Gerlai, Lahav, Guo, & Rosenthal, 2000; Zabegalov et al., 2019), both highly relevant to ICDs (Figure 2 and Table 2). Zebrafish possess fully sequenced genome with >70% homology to human genes (Howe et al., 2013), shared neurotransmitter systems, well‐conserved brain circuits (Stewart, Ullmann, et al, 2015) and a potential for high‐throughput in vivo drug screening (Li et al., 2017; Macrae & Peterson, 2015; Stewart, Gerlai, & Kalueff, 2015). Zebrafish also offer several genetic and epigenetic models (Huang et al., 2015; Lakstygal, de Abreu, & Kalueff, 2018; Lange et al., 2012) that can contribute to a better understanding of ICD pathobiology.…”

Section: Introductionmentioning

confidence: 99%

Zebrafish models of impulsivity and impulse control disorders

Abreu

Giacomini

Genário

et al. 2020

Eur J of Neuroscience

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Impulse control disorders (ICDs) are characterized by generalized difficulty controlling emotions and behaviors. ICDs are a broad group of the central nervous system (CNS) disorders including conduct disorder, intermittent explosive, oppositional‐defiant disorder, antisocial personality disorder, kleptomania, pyromania and other illnesses. Although they all share a common feature (aberrant impulsivity), their pathobiology is complex and poorly understood. There are also currently no ICD‐specific therapies to treat these illnesses. Animal models are a valuable tool for studying ICD pathobiology and potential therapies. The zebrafish (Danio rerio) has become a useful model organism to study CNS disorders due to high genetic and physiological homology to mammals, and sensitivity to various pharmacological and genetic manipulations. Here, we summarize experimental models of impulsivity and ICD in zebrafish and highlight their growing translational significance. We also emphasize the need for further development of zebrafish ICD models to improve our understanding of their pathogenesis and to search for novel therapeutic treatments.

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Zebrafish: A promising in vivo model for assessing the delivery of natural products, fluorescence dyes and drugs across the blood-brain barrier

Cited by 58 publications

References 96 publications

Steroid Transport, Local Synthesis, and Signaling within the Brain: Roles in Neurogenesis, Neuroprotection, and Sexual Behaviors

Steroid Transport, Local Synthesis, and Signaling within the Brain: Roles in Neurogenesis, Neuroprotection, and Sexual Behaviors

Streamlining drug discovery assays for cardiovascular disease using zebrafish

Zebrafish models of impulsivity and impulse control disorders

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