Zebrafish: A Model for the Study of Toxicants Affecting Muscle Development and Function

Dubińska–Magiera, Magda; Daczewska, Małgorzata; Lewicka, Anna; Migocka-Patrzałek, Marta; Niedbalska-Tarnowska, Joanna; Jagla, Krzysztof

doi:10.3390/ijms17111941

Cited by 55 publications

(20 citation statements)

References 160 publications

(179 reference statements)

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“…Many biochemical pathways also share high similarity, which facilitates the interpretation of test results from zebrafish. It is also worth noting that zebrafish genes associated with human diseases share a high level of conservation (84%) [ 78 , 79 ]. Therefore the zebrafish model has been used successfully in the modeling of many human diseases, including genetic and metabolic disorders [ 80 , 81 , 82 ].…”

Section: Why Use Zebrafish To Study Pygm?mentioning

confidence: 99%

Muscle Glycogen Phosphorylase and Its Functional Partners in Health and Disease

Migocka-Patrzałek

Elías

2021

Cells

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Glycogen phosphorylase (PG) is a key enzyme taking part in the first step of glycogenolysis. Muscle glycogen phosphorylase (PYGM) differs from other PG isoforms in expression pattern and biochemical properties. The main role of PYGM is providing sufficient energy for muscle contraction. However, it is expressed in tissues other than muscle, such as the brain, lymphoid tissues, and blood. PYGM is important not only in glycogen metabolism, but also in such diverse processes as the insulin and glucagon signaling pathway, insulin resistance, necroptosis, immune response, and phototransduction. PYGM is implicated in several pathological states, such as muscle glycogen phosphorylase deficiency (McArdle disease), schizophrenia, and cancer. Here we attempt to analyze the available data regarding the protein partners of PYGM to shed light on its possible interactions and functions. We also underline the potential for zebrafish to become a convenient and applicable model to study PYGM functions, especially because of its unique features that can complement data obtained from other approaches.

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Section: Why Use Zebrafish To Study Pygm?mentioning

confidence: 99%

Muscle Glycogen Phosphorylase and Its Functional Partners in Health and Disease

Migocka-Patrzałek

Elías

2021

Cells

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“…The embryo is sensitive and has specific consideration for its development. The unfavorable condition can literally cause stress to the embryo affecting its growth and development stage resulting in morphological abnormalities [12]. One of the common morphological abnormalities is scoliosis.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Effect of AqueousMomordica charantiaLinn. Extract on Zebrafish Embryo Model through Acute Toxicity Assay Assessment

Thiagarajan

Krishnan

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Momordica charantia Linn., commonly known as bitter gourd, has many protective roles due to its medicinal value as it contains bioactive components. However, this extract showed possible toxicity effect on zebrafish embryo. Thus this study was designed to differentiate the toxicity activities in two types of M. charantia sample which are Indian and Chinese M. charantia, as well as to compare between two different aqueous extraction methods, hot and cold aqueous method, using zebrafish embryo assay assessment. It was observed that the survival rate of zebrafish embryo decreased as the concentration of test extract increased for all samples of M. charantia. The LC50 values of hot aqueous Chinese M. charantia, hot aqueous Indian M. charantia, and cold aqueous Chinese M. charantia were 144.54 μg/ml, 199.53 μg/ml, and 251.19 μg/ml, respectively. However, cold aqueous Indian M. charantia has a higher LC50 which was not in the range of the tested concentration. Hatchability of Danio rerio embryo reduced as the concentration of M. charantia extract increased while no hatching was observed in the highest concentration (1000 μg/ml). Scoliosis of zebrafish larvae was only seen in higher concentrations (125-1000 μg/ml) of extract. The heartbeat of zebrafish larvae treated with M. charantia extract was within the normal range, 120-180 bpm, but at higher concentrations (125-1000 μg/ml) the heartbeat differed for all samples of test extract. Hence, although this plant extract was safe to be consumed due to its pharmaceutical effect, it still exhibited mild toxicity effect at higher concentration when it was evaluated on zebrafish embryo.

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“…The tissue evaluation was performed qualitatively, alterations such as pericardial edema, yolk sac edema, skeletal deformations, defined eye, and axial malformations (abnormal notochord) were verified. These elements are part of the most sensitive acute embryo and larvae stage sub-lethal endpoints ( Anuradha and Katti, 2009 , Dubinska-Magiera et al, 2016 ).…”

Section: Methodsmentioning

confidence: 99%

Antibacterial activity of Lemna minor extracts against Pseudomonas fluorescens and safety evaluation in a zebrafish model

González-Rentería

Monroy-Dosta

Guzmán-García

et al. 2020

Saudi Journal of Biological Sciences

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The treatment of bacterial diseases in aquaculture is done using antibiotics, their applications has resulted in contamination and bacterial resistance. Natural extracts are a potential alternative as an antimicrobial, they have demonstrated effectiveness in their use aimed at treating conditions. The purpose of this study was to evaluate the antimicrobial activity of Lemna minor extracts against Pseudomonas fluorescens with different solvent for extraction. Methanol, chloroform and hexane were used. Subsequently, the safety assessment of the extracts in Danio rerio embryos and larvae was performed to validate as ecologically harmless. Antibacterial activity was detected in three extracts with significant differences (p = 0.001). Hexane extract had the highest antibacterial activity, followed by chloroform and methanol extracts. The three extracts have differences with respect to the control, between times and concentrations tested (p = 0.001). Minimum inhibitory concentration values (MIC) at 24 h methanolic extract ME 0.05 µg mL −1 . In embryos and larvae increased safety of the LC 50 methanolic extract was evidenced followed by the hexane and chloroform extract. No morphological or tissue changes were observed in embryos and larvae. The hexane extracts of L. minor had a greater bactericidal effect against P. fluorescens and are functional because of their antibacterial activity, but methanolic extract is more safety in embryos and larvae of D. rerio , making it a potential alternative for use in the treatment and control of septicemia in fish.

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Zebrafish: A Model for the Study of Toxicants Affecting Muscle Development and Function

Cited by 55 publications

References 160 publications

Muscle Glycogen Phosphorylase and Its Functional Partners in Health and Disease

Muscle Glycogen Phosphorylase and Its Functional Partners in Health and Disease

Evaluation of the Effect of AqueousMomordica charantiaLinn. Extract on Zebrafish Embryo Model through Acute Toxicity Assay Assessment

Antibacterial activity of Lemna minor extracts against Pseudomonas fluorescens and safety evaluation in a zebrafish model

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