Zastosowanie testów toksykologicznych w przemyśle naftowym

Steliga, Teresa; Badawczy, Instytut Nafty i Gazu – Państwowy Instytut; Jakubowicz, P.; Wojtowicz, Katarzyna; Kluk, D.

doi:10.18668/ng.2018.09.07

Cited by 3 publications

(4 citation statements)

References 10 publications

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“…The result of the Microtox ® Solid Phase test is EC 50 , which is the concentration of the test sample, which produces 50% of the test reaction. To assess environmental risk, the MARA test was used, based on evaluating the degree of growth inhibition of 18 test organisms (Microbacterium spaciec, Brevundimonas diminuta, Citrobacter freudii, Comamonas testosteroni, Entrococcus casseliflavus, Delftia acidovorans, Kurthia gibsoni, Staphylococcus warneri, Pseudomonas aurantiaca, Serriatia rudidaea and Pichia anomala) after 18 h of incubation [17,62,65,69]. All toxicological tests were conducted in accordance with the manufacturers' procedures, while the results obtained were converted to TU values.…”

Section: Soil Toxicity Analysismentioning

confidence: 99%

Evaluation of the Effectiveness of Bioaugmentation-Assisted Phytoremediation of Soils Contaminated with Petroleum Hydrocarbons Using Echinacea purpurea

Wojtowicz,

Steliga,

Kapusta

2023

Applied Sciences

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Phytoremediation supported by bioaugmentation is a promising method considered for cleaning up areas polluted with petroleum hydrocarbons. In this study, phytoremediation was carried out using Echinacea purpurea as a phytoremediant on two types of soil: Soil DW—aged soil taken from an excavation pit, Soil OS—soil taken from an oil spill area. The tests for each soil were carried out in six test systems (non-inoculation, inoculation with the B1 microbial consortium, inoculation with the B2 microbial consortium, inoculation with the B1 microbial consortium with the addition of γ-PGA (γ-poly glutamic acid), inoculation with the B2 microbial consortium with the addition of γ-PGA and inoculation with the γ-PGA solution) for 6 months. The effectiveness of the remediation treatments used was assessed based on chromatographic analyses of soil and plant material (roots, shoots) and toxicological analyses using four types of toxicological tests (PhytotoxkitTM (MicroBioTests Inc., Gent, Belgium), OstracodtoxkitTM (MicroBioTests Inc., Gent, Belgium), Microtox® Solid Phase Test (Modern Water Inc., New Castle, DE, USA), MARA (NCIMB Ltd., Aberdeen, UK)). The research conducted showed that the most effective method of bioremediation of soils contaminated with petroleum hydrocarbons was phytoremediation supported by bioaugmentation with the microbial consortium B2 with γ-PGA, which allowed for reducing the concentration of total petroleum hydrocarbons (TPHs) and polycyclic aromatic hydrocarbons (PAHs) in the tested soils by 53.98% and 49.54% (Soil DW-5) and 60.47% and 37.55% (Soil OS-5), respectively. In turn, the lowest bioremediation efficiency was recorded in non-inoculated systems, for which the concentration of TPHs and PAHs at the end of the study decreased by 18.40% and 16.14% (Soil DW-1) and 21.87% and 18.20% (Soil OS-1), respectively. The results of toxicological analyses confirmed the relationship between the concentration of TPHs and PAHs in the soil and its toxicity level.

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Section: Soil Toxicity Analysismentioning

confidence: 99%

Evaluation of the Effectiveness of Bioaugmentation-Assisted Phytoremediation of Soils Contaminated with Petroleum Hydrocarbons Using Echinacea purpurea

Wojtowicz,

Steliga,

Kapusta

2023

Applied Sciences

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“…The effectiveness of biodegradation processes carried out in the soil, parallel to determination of toxicant (PCBs) concentration, was assessed based on results of toxicological tests. Soil samples were analysed using 3 micro-biotests with organisms belonging to three trophic levels: producers; Phytotoxkit TM (Maminidy- Pajany et al, 2011;Blinova et al, 2012;Baran and Tarnawski, 2013), consumers; Ostracodtoxkit F TM (Niyommaneerat et al, 2017, Steliga et al, 2018b, reducers; Microtox ® STP (Lima et al, 2011;Slater et al, 2011;Trusz-Zdybek et al, 2012;Foucaulty et al, 2013;Oleszczuk et al, 2014;Oh et al, 2015) and additional mutagenicity test Ames (Steliga et al, 2012;Vijay et al, 2018).…”

Section: Toxicological Analysismentioning

confidence: 99%

“…A comprehensive assessment of the biodegradation effectiveness should be based not only on the concentration of selected physical and chemical parameters, but should also include a toxicological analysis. Toxicological tests allow simultaneous determination of harmful action of all substances present in the tested sample on selected live organisms, taking into account interactions occurring between all components of the tested system(Steliga et al, 2018b).…”

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confidence: 99%

Study on bioremediation of soil contaminated with polychlorinated biphenyls (PCBs)

Wojtowicz

Steliga

2020

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The article presents issues related to biodegradation of polychlorinated biphenyls in soil by inoculation with a biopreparation IN-3 developed on the basis of microorganisms capable of metabolizing PCB. Biodegradation tests were carried out using the ex-situ technological pile method on a specially constructed test stand. As part of the research, physical and chemical analyzes of the initial soil were performed, with particular regard to the content of nutrients (nitrogen and phosphorus), PCB chromatographic analyzes and toxicological analyzes. Before inoculating soil with biopreparation IN-3, the parameters of the process were established (temperature = 17-25°C, humidity = 20-25%, pH = 7.5-7.8, content of nutrients: C:N:P = 100:10:1), which were monitored during bioremediation. During soil treatment, the content of identified PCB congeners was monitored. The assessment of biodegradation efficiency of polychlorinated biphenyls in soil was complemented by toxicological monitoring, which was carried out using live organisms representing all trophic levels: producers (Phytotoxkit TM test), consumers (Ostracodtoxkit F TM test) and reducers agents (Microtox ® SPT test). Also, the presence of mutagenic factors were tested with the Ames mutagenicity test. As a result of the six-month biodegradation process of soil contaminated with aged transformer oil, upon inoculation with biopreparation IN-3, a reduction in the content of polychlorinated biphenyls from 13 153.9 µg/kg d.m. down to 1650.4 µg/kg d.m. (87.5%) was archieved. The obtained chromatographic analysis results showed that the efficiency of the PCB biodegradation depended on the number of chlorine atoms in the biphenyl ring (with the increase in the number of chlorine atoms in the PCB molecule the degree of biodegradation decreased) and the structure of the molecule (PCB congeners having 2 or more chlorine atoms in the biphenyl ring ortho positions were more difficult to biodegrade than non-ortho or mono-ortho, while polychlorinated biphenyls, in which chlorine atoms occur on only one phenyl ring, were more easily biodegradable). The toxicological analysis showed a reduction in the toxicity of the soil tested from toxicity unit TU = 26.7 to TU = 6.1. In addition, toxkit tests (Phytotoxkit TM and Ostracodtoxkit F TM ) showed a decrease in inhibition of test organism growth from 69.3 to 14.8% (Ostracodtoxkit F TM test) and from 64.7-78.0 to 11.9-17.5% (Phytotoxkit test). Decreasing the mutagenicity index from 8.3 to 1.5 in the Ames test confirmed the effectiveness of the purification process. Based on the results of the experiment, conclusions were made regarding issues related to biodegradation of polychlorinated biphenyls in soil.

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“…On the other hand, tests which use invertebrates and embryophyta as bioindicators include tests of the type of ToxKit (Phytotoxkit TM , Spirodela Duckweed Toxkit, Ostracodtoxkit F TM ). Bioassays have the advantage of being able to determine the amount of the pollutant that causes acute or chronic toxicity, which in turn allows the estimation on the effects of organisms exposure to a given substance (Gabrielson et al, 2003;Sekutowski and Bartniak, 2009;Fai and Grant, 2010;Zima, 2012;Foucault et al, 2013;Jakubowicz et al, 2013;Oleszczuk et al, 2014;Niyommaneerat et al, 2017;Steliga et al, 2018).…”

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confidence: 99%

Potential of Helianthus annuus for phytoremediation of lead, zinc, total petroleum hydrocarbons (TPH) and polycyclic aromatic hydrocarbons (PAHs) contaminated soil

Kluk¹,

Steliga²

2019

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This paper describes the results regarding effectiveness of treatment of soils contaminated with heavy metals (lead, zinc), total petroleum hydrocarbons (TPH) and polycyclic aromatic hydrocarbons (PAHs): naphthalene (Nap), phenanthrene (Phe), fluoranthene (Flt), chrysene(Chr), in the process of phytoremediation carried out a laboratory scale with the plant Helianthus annuus. The reason behind the work was to investigate how plant vegetation can reduce the concentration of heavy metals in the soil and how the presence of petroleum substances and polycyclic aromatic hydrocarbons affect the efficiency of their removal. The analysis was based on the dynamics of cleaning the contaminated soils which contained periodic testing on physical, chemical and toxicological properties of: uncontaminated soil, soil contaminated with TPH and PAHs, soil contaminated with heavy metals, soil contaminated with heavy metals and petroleum substances. Phytoremediation of soils and its effectiveness in pot experiments was assessed based on physical and chemical soil analyses as well as five microbiotests applying organisms belonging to three trophic levels: producers (Phytotoxkit TM and Spirodela Duckweed Toxkit), consumers (Ostracodtoxkit TM) and decomposers (Microtox ® STP, MARA). The conducted research has shown that combining the results of chemical analyzes and biological tests on contaminated soils enable a real ecotoxicological assessment of environmental hazards. Six-month long phytoremediation of soils contaminated with heavy metals (Pb, Zn), and heavy metals together with petroleum substances (TPH, PAHs), yielded results in terms of a content reduction of: lead (from of 472-495 mg/ kg dry mass of soil to 226-318 mg/kg d.m.), zinc (from 998-1030 mg/kg d.m. to 520-656 mg/kg d.m.), TPH (from 4806-4824 mg/kg d.m. to 2774-3180 mg/kg d.m.) and PAH (removal rates of Nap: at 28,0-29.9%, Phe: at 25.1-27.0%, Flt: at 24-26,3%, Chr: at 14.5-18.6%). After finishing the experiment the content of lead and zinc in the plant biomass was also measured. Based on the obtained results, conclusions were made regarding issues related to usage of phytoremediation to clean contaminated soils depending on the concentration and type of toxic substance.

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Zastosowanie testów toksykologicznych w przemyśle naftowym

Cited by 3 publications

References 10 publications

Evaluation of the Effectiveness of Bioaugmentation-Assisted Phytoremediation of Soils Contaminated with Petroleum Hydrocarbons Using Echinacea purpurea

Evaluation of the Effectiveness of Bioaugmentation-Assisted Phytoremediation of Soils Contaminated with Petroleum Hydrocarbons Using Echinacea purpurea

Study on bioremediation of soil contaminated with polychlorinated biphenyls (PCBs)

Potential of Helianthus annuus for phytoremediation of lead, zinc, total petroleum hydrocarbons (TPH) and polycyclic aromatic hydrocarbons (PAHs) contaminated soil

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