Zastosowanie kliniczne szybkiego testu skryningowego opartego o technologię kapilarnej elektroforezy strefowej (CZE) do identyfikacji infekcji Escherichia coli w materiale biologicznym.

Abstract

SummaryBackgroundEscherichia coli is a Gram-negative bacterium which is a basic, symbiotic element of the physiological flora of the large intestine of humans and warm-blooded animals. However, in specific cases it may become a very dangerous pathogen (eg, diarrhoea, infection of the urinary tract, lungs, and generalized infections). Its early detection, as a cause of infectious disease, helps to achieve optimal treatment results; however, classical microbiological tests require at least 24 hours from sample taking to diagnosis.Material/MethodsWe present a unique solution based on CZE technologies enabling identification of E. coli presence in studied sample within half an hour. Altogether, 30 E. coli-infected wounds and ulcerations were examined, comparing the results obtained by classical culture method with the result of capillary zone electrophoresis (CZE) electropherogram.ResultsThe method, which does not require any preparation of the sample, achieved 86.7% sensitivity and 85%specificity in the examined clinical material (infections of surgical wounds).ConclusionsThe obtained results enable reliable, very fast testing for E. coli as a pathogen.

A study of interactions between bacteria and antibiotics by capillary electrophoresis

“…Our previous examinations and tests were focused only on marking the bacterial cells directly in the biological material . This time we tried to evaluate the effectiveness of treatments with antibiotics.…”

“…Usually, the standard procedure after confirmation of wound infection is the routine application of typical antibiotics, most often without culturing the pathogen. It is the main reason why we try to adopt electromigration techniques in the fast identification of bacteria in wound infection .…”

“…In our previous study, we described a clinical application of a rapid microbiological test based on CZE to access local skin infection and we proposed a fast screening CZE‐based test for identification of bacteria in biological material . In 2002, Armstrong and coworkers applied CZE to evaluate the binding of antibiotic (vancomycin) to the Bifidobacterium infantis cells.…”

Capillary electrophoresis in the diagnosis of surgical site infections

“…The sample preparation and measurements were carried out on the basis of the procedures described in our earlier work . Electropherograms obtained for the biological samples and standards were compared under the same conditions.…”

“…For individual bacterial cell types, as well as components presented in the sample matrix, a characteristic spectrum that allowed for the identification of signals and a qualitative assessment of pathogens in biological samples were obtained. At this stage, the identification could be derived from the spectra of Gram (+) and Gram (−), and the background of the sample (matrix) [3,4]. Sample preparation is not required, which considerably speeds up and simplifies the determination of bacteria in a biological matrix.…”