A study of interactions between bacteria and antibiotics by capillary electrophoresis

Kłodzińska, Ewa; Jaworski, Maciej; Kupczyk, Wojciech; Jackowski, Marek; Buszewski, Bogusław

doi:10.1002/elps.201200281

Cited by 11 publications

(9 citation statements)

References 8 publications

(14 reference statements)

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“…The goal of the present study was to investigate antibacterial activity of BioAgNPs synthesized by a new Actinomycetes CGG11n strain and their synergistic effect against Gram (–) and Gram (+) bacteria using fluorescence flow cytometry method as an alternative to the standard ones and as a complementary method to electromigration techniques . Moreover, interaction of different concentrations of silver nanoparticles in combination with various commercial antibiotics was evaluated.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial properties of biosynthesized silver nanoparticles studied by flow cytometry and related techniques

et al. 2016

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This work reports the effect of silver bionanoparticles (Bio(AgNPs) synthesized by Actinobacteria CGG 11n on selected Gram (+) and Gram (-) bacteria. Flow cytometry, classical antibiogram method and fluorescent microscopy approach was used for evaluation of antimicrobial activity of Bio(AgNPs) and their combination with antibiotics. Furthermore, the performed research specified the capacity of flow cytometry method as an alternative to the standard ones and as a complementary method to electromigration techniques. The study showed antibacterial activity of both BioAgNPs and the combination of antibiotics/BioAgNPs against all the tested bacteria strains in comparison with a diffusion, dilution and bioautographic methods. The synergistic effect of antibiotics/BioAgNPs combination (e.g. kanamycin, ampicillin, neomycin and streptomycin) was found to be more notable against Pseudomonas aeruginosa representing a prototype of multi-drug resistant "superbugs" for which effective therapeutic options are very limited.

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Section: Introductionmentioning

confidence: 99%

Antimicrobial properties of biosynthesized silver nanoparticles studied by flow cytometry and related techniques

et al. 2016

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“…In further studies, we undertake attempts to develop weekly individual profiles for patients after application of antibiotics. This was done in order to show the impact of antibiotic therapy in change “numbers” of bacteria present in the wound after surgery .…”

Section: Methodsmentioning

confidence: 99%

Capillary electrophoresis in the diagnosis of surgical site infections

et al. 2013

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A surgical site infection (SSI) is an infection that occurs after surgery in the part of the body where the surgery took place. A SSI may range from a spontaneously limited wound discharge within 7-10 days of an operation to a life-threatening postoperative complication, such as a sternal infection after open heart surgery. Most surgical site infections are caused by contamination of an incision with microorganisms from the patient's own body during surgery. From the analytical point of view the complex natures of these samples as well as the low concentrations of analytes require a system with high sensitivity and efficiency. Such situation require a technique such as capillary electrophoresis which is a powerful and versatile separation technique that promises to rival high performance liquid chromatography when applied to the separation of both charged and neutral species. During the study, it has been demonstrated that CZE identifying characteristics of such groups of pathogens such as bacteria G (+) and different species of bacteria G (-) and also develop weekly individual profiles for patients after application of antibiotics. This was done in order to show the impact of antibiotic therapy in change "numbers" of bacteria present in the wound after surgery. The method proved to be the ideal straight specificity in the case of E. coli (100%). Finally analysis of the spectra and the second derivatives of the UV-Vis spectra confirmed the similarity in the profiles and showed that the CZE is a great method for fast screening test in bacterial infection.

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“…CE detection of microorganisms can be based on the detection of cells [10][11][12][13][14][15][16][17][18], proteins [19][20][21], liposomes [22], or nucleic acids. Among them, nucleic acid detection eliminates many types of false positive and negative results, offering a more precise diagnosis.…”

Section: Diagnosing Infectious Diseases With Cementioning

confidence: 99%

Capillary electrophoresis based on nucleic acid detection for diagnosing human infectious disease

Lian

Zhao

2016

Clinical Chemistry and Laboratory Medicine (CCLM)

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Rapid transmission, high morbidity, and mortality are the features of human infectious diseases caused by microorganisms, such as bacteria, fungi, and viruses. These diseases may lead within a short period of time to great personal and property losses, especially in regions where sanitation is poor. Thus, rapid diagnoses are vital for the prevention and therapeutic intervention of human infectious diseases. Several conventional methods are often used to diagnose infectious diseases, e.g. methods based on cultures or morphology, or biochemical tests based on metabonomics. Although traditional methods are considered gold standards and are used most frequently, they are laborious, time consuming, and tedious and cannot meet the demand for rapid diagnoses. Disease diagnosis using capillary electrophoresis methods has the advantages of high efficiency, high throughput, and high speed, and coupled with the different nucleic acid detection strategies overcomes the drawbacks of traditional identification methods, precluding many types of false positive and negative results. Therefore, this review focuses on the application of capillary electrophoresis based on nucleic detection to the diagnosis of human infectious diseases, and offers an introduction to the limitations, advantages, and future developments of this approach.

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A study of interactions between bacteria and antibiotics by capillary electrophoresis

Cited by 11 publications

References 8 publications

Antimicrobial properties of biosynthesized silver nanoparticles studied by flow cytometry and related techniques

Antimicrobial properties of biosynthesized silver nanoparticles studied by flow cytometry and related techniques

Capillary electrophoresis in the diagnosis of surgical site infections

Capillary electrophoresis based on nucleic acid detection for diagnosing human infectious disease

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