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Sibmooh, Nathawut; Yamanont, Paveena; Krudsood, Srivicha; Leowattana, Wattana; Brittenham, Gary M.; Looareesuwan, Sornchai; Udomsangpetch, Rachanee

doi:10.1186/1476-511x-3-15

Cited by 31 publications

(16 citation statements)

References 28 publications

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“…Additionally, it is likely that the parasite modifies metabolic pathways of lipids in the hepatocytes. Finally, oxidative stress has been associated with oxidation of lipoproteins, contributing to the abnormalities of lipids levels [17, 24, 26]. …”

Section: Discussionmentioning

confidence: 99%

“…In fact, a high proportion of children with malaria present HDL and LDL levels below normal range. The mechanisms by which inflammation and infection decrease HDL levels are uncertain, but in acute malaria two factors contribute for these findings: the redox imbalance which favors the oxidation of lipoproteins; the mobilization of cholesterol internalized by lipoproteins by Plasmodium [17, 23, 26]. Additionally, the occurrence of functional alterations of these lipoproteins is likely, as seen in other inflammatory disorders, such as the diminished ability of HDL to prevent the oxidation of LDL [8].…”

Section: Discussionmentioning

confidence: 99%

“…There is a paucity of data describing lipid abnormalities in children with vivax malaria. Such changes can increase the risk of chronic diseases, as atherosclerosis and related diseases, which is relevant for children continuously exposed to malaria [14, 16, 17]. Additionally, data correlating the parasitaemia at admission or the history of malaria with lipid levels are inconclusive in children [10].…”

Section: Introductionmentioning

confidence: 99%

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Lipid Profile of Children with Malaria byPlasmodium vivax

Dias

Vieira

Cabral

et al. 2016

Journal of Tropical Medicine

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Background. Changes in lipid profile are commonly reported in adult patients with malaria. However, a few studies evaluated lipid abnormalities in children continuously exposed to P. vivax. Objective. To evaluate lipid abnormalities in children with P. vivax infection and to assess if parasite count or the history of malaria correlates with lipid levels at admission. Methods. A total of 75 children were included in the study, from which 43 were slide confirmed infection by P. vivax. Serial blood samples were collected at admission and, on days 7 and 14, evaluated for the colorimetric measurements of triglycerides, very low-density lipoprotein (VLDL), total cholesterol, high-density lipoprotein (HDL), and low-density lipoprotein (LDL). Results. The levels of total cholesterol, LDL, and HDL were significantly lower in malaria cases. The levels of VLDL and triglycerides were significantly higher in children with malaria. Such changes were transient and were not associated with parasite counting as well as with the history of malaria of patients. Conclusion. There are significant lipid abnormalities in children with low level of P. vivax infection and mild signs and symptoms of the disease, which are not associated with parasitaemia and previous episodes of disease.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Lipid Profile of Children with Malaria byPlasmodium vivax

Dias

Vieira

Cabral

et al. 2016

Journal of Tropical Medicine

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“…However, we show here that hGIIA is inactive against P. falciparum at concentrations as high as 1.5 M under standard culture conditions, which suggests that its role in malaria, if any, might rather relate to inflammatory response of the host than direct inhibition of parasite development. However, it has been shown that hGIIA sPLA 2 exhibits enhanced potency toward HDL from acute-phase serum (56) and in vitro-oxidized lipoproteins (57), which raises the possibility that this sPLA 2 might be active against Plasmodium under pathophysiological conditions when lipoproteins get oxidized (58).…”

Section: Figmentioning

confidence: 99%

In Vitro Anti-Plasmodium falciparum Properties of the Full Set of Human Secreted Phospholipases A ₂

et al. 2015

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We have previously shown that secreted phospholipases A 2 (sPLA 2 s) from animal venoms inhibit the in vitro development of Plasmodium falciparum, the agent of malaria. In addition, the inflammatory-type human group IIA (hGIIA) sPLA 2 circulates at high levels in the serum of malaria patients. However, the role of the different human sPLA 2 s in host defense against P. falciparum has not been investigated. We show here that 4 out of 10 human sPLA 2 s, namely, hGX, hGIIF, hGIII, and hGV, exhibit potent in vitro anti-Plasmodium properties with half-maximal inhibitory concentrations (IC 50 s) of 2.9 ؎ 2.4, 10.7 ؎ 2.1, 16.5 ؎ 9.7, and 94.2 ؎ 41.9 nM, respectively. Other human sPLA2s, including hGIIA, are inactive. The inhibition is dependent on sPLA 2 catalytic activity and primarily due to hydrolysis of plasma lipoproteins from the parasite culture. Accordingly, purified lipoproteins that have been prehydrolyzed by hGX, hGIIF, hGIII, and hGV are more toxic to P. falciparum than native lipoproteins. However, the total enzymatic activities of human sPLA 2 s on purified lipoproteins or plasma did not reflect their inhibitory activities on P. falciparum. For instance, hGIIF is 9-fold more toxic than hGV but releases a lower quantity of nonesterified fatty acids (NEFAs). Lipidomic analyses of released NEFAs from lipoproteins demonstrate that sPLA 2 s with anti-Plasmodium properties are those that release polyunsaturated fatty acids (PUFAs), with hGIIF being the most selective enzyme. NEFAs purified from lipoproteins hydrolyzed by hGIIF were more potent at inhibiting P. falciparum than those from hGV, and PUFA-enriched liposomes hydrolyzed by sPLA 2 s were highly toxic, demonstrating the critical role of PUFAs. The selectivity of sPLA 2 s toward lowand high-density (LDL and HDL, respectively) lipoproteins and their ability to directly attack parasitized erythrocytes further explain their anti-Plasmodium activity. Together, our findings indicate that 4 human sPLA 2 s are active against P. falciparum in vitro and pave the way to future investigations on their in vivo contribution in malaria pathophysiology.

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“…Measurements of resonance energy transfer from tryptophan of the LDL particles to pyrene and pyrene monomer-excimer formation was used to determine the annular and bulk fluidity respectively (Avdulov et al, 1997; Hashimoto et al, 2001; Sibmooh et al, 2004). Briefly, 10 mM PBS (pH 7.4) was added to the LDL samples to make a final volume of 2 mL containing 50 μg/ml protein.…”

Section: Methodsmentioning

confidence: 99%

Elucidating the structural organization of a novel low-density lipoprotein nanoparticle reconstituted with docosahexaenoic acid

Mulik

Zheng

Pichumani

et al. 2017

Chemistry and Physics of Lipids

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Low-density lipoprotein nanoparticles reconstituted with unesterified docosahexaenoic acid (LDL-DHA) is promising nanomedicine with enhanced physicochemical stability and selective anticancer cytotoxic activity. The unique functionality of LDL-DHA ultimately relates to the structure of this nanoparticle. To date, however, little is known about the structural organization of this nanoparticle. In this study chemical, spectroscopic and electron microscopy analyses were undertaken to elucidate the structural and molecular organization of LDL-DHA nanoparticles. Unesterified DHA preferentially incorporates into the outer surface layer of LDL, where in this orientation the anionic carboxyl end of DHA is exposed to the LDL surface and imparts an electronegative charge to the nanoparticles surface. This negative surface charge promotes the monodisperse and homogeneous distribution of LDL-DHA nanoparticles in solution. Further structural analyses with cryo-electron microscopy revealed that the LDL-DHA nanostructure consist of a phospholipid bilayer surrounding an aqueous core, which is distinctly different from the phospholipid monolayer/apolar core organization of plasma LDL. Lastly, apolipoprotein B-100 remains strongly associated with this complex and maintains a discrete size and shape of the LDL-DHA nanoparticles similar to plasma LDL. This preliminary structural assessment of LDL-DHA now affords the opportunity to understand the important structure-function relationships of this novel nanoparticle.

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Cited by 31 publications

References 28 publications

Lipid Profile of Children with Malaria byPlasmodium vivax

Lipid Profile of Children with Malaria byPlasmodium vivax

In Vitro Anti-Plasmodium falciparum Properties of the Full Set of Human Secreted Phospholipases A ₂

Elucidating the structural organization of a novel low-density lipoprotein nanoparticle reconstituted with docosahexaenoic acid

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Untitled

Cited by 31 publications

References 28 publications

Lipid Profile of Children with Malaria byPlasmodium vivax

Lipid Profile of Children with Malaria byPlasmodium vivax

In Vitro Anti-Plasmodium falciparum Properties of the Full Set of Human Secreted Phospholipases A 2

Elucidating the structural organization of a novel low-density lipoprotein nanoparticle reconstituted with docosahexaenoic acid

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In Vitro Anti-Plasmodium falciparum Properties of the Full Set of Human Secreted Phospholipases A ₂