Untitled

Abstract

Previous studies have reported that mesenchymal stem cells (MSC) may be isolated from the synovial membrane by the same protocol as that used for synovial fibroblast cultivation, suggesting that MSC correspond to a subset of the adherent cell population, as MSC from the stromal compartment of the bone marrow (BM). The aims of the present study were, first, to better characterize the MSC derived from the synovial membrane and, second, to compare systematically, in parallel, the MSC-containing cell populations isolated from BM and those derived from the synovium, using quantitative assays. Fluorescent-activated cell sorting analysis revealed that both populations were negative for CD14, CD34 and CD45 expression and that both displayed equal levels of CD44, CD73, CD90 and CD105, a phenotype currently known to be characteristic of BM-MSC. Comparable with BM-MSC, such MSC-like cells isolated from the synovial membrane were shown for the first time to suppress the T-cell response in a mixed lymphocyte reaction, and to express the enzyme indoleamine 2,3-dioxygenase activity to the same extent as BM-MSC, which is a possible mediator of this suppressive activity. Using quantitative RT-PCR these data show that MSC-like cells from the synovium and BM may be induced to chondrogenic differentiation and, to a lesser extent, to osteogenic differentiation, but the osteogenic capacities of the synoviumderived MSC were significantly reduced based on the expression of the markers tested (collagen type II and aggrecan or alkaline phosphatase and osteocalcin, respectively). Transcription profiles, determined with the Atlas Human Cytokine/Receptor Array, revealed discrimination between the MSC-like cells from the synovial membrane and the BM-MSC by 46 of 268 genes. In particular, activin A was shown to be one major upregulated factor, highly secreted by BM-MSC. Whether this reflects a different cellular phenotype, a different amount of MSC in the synovium-derived population compared with BM-MSC adherent cell populations or the impact of a different microenvironment remains to be determined. In conclusion, although the BM-derived and synovium-derived MSC shared similar phenotypic and functional properties, both their differentiation capacities and transcriptional profiles permit one to discriminate the cell populations according to their tissue origin.

Regeneration of the articular surface of the rabbit synovial joint by cell homing: a proof of concept study

“…Cells from regenerated cartilage were spherical, but assumed spindle shape after 7-day culture, whereas cells from regenerated bone maintained their initial spindle shape. Activin A, a marker preferentially expressed by bone marrow cells,37 showed little expression in cells from regenerated cartilage, but was robustly expressed in cells from regenerated bone (webappendix p 6). Furthermore TGFβ3 recruited a remarkable number of mesenchymal stem or progenitor cells and synovium stem or progenitor cells in vitro by comparison with spontaneous cell migration (webappendix p 7).…”

Differentiation of Bone Marrow Stroma-Derived Mesenchymal Cells

“…In contrast, other reports claim that CD34 and CD62 are not expressed by MSCs [11,33,34]. Additional antigens that are recognized as markers of progenitors of the marrow stromal cells are CD166, CD105, CD73, and CD63 [34][35][36][37][38][39]. CD166, activated leukocyte cell adhesion molecule (ALCAM), and osteopontin are also involved in MSC-niche interactions [40][41][42][43].…”

Mesenchymal stem cells, autoimmunity and rheumatoid arthritis

“…One documented mechanism by which S-MSC can display their suppression effect on T cells is via the production of IDO 39 (Figure 2, A). It has been shown that S-MSC and BM-MSC can display similar IDO activity at a basal level or when induced by IFN-γ and TNF-α 39 . Therefore, a similar inhibitory effect by S-MSC could be expected on other immune cells.…”