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Oberhagemann, Petra; Chatot-Balandras, Catherine; Schäfer-Pregl, Ralf; Wegener, Dorothee; Palomino, Carmen; Salamini, F.; Bonnel, Eric; Gebhardt, Christiane

doi:10.1023/a:1009623212180

Cited by 144 publications

(22 citation statements)

References 30 publications

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“…In this study, for the first time, a high-throughput simplified 2b-RAD sequencing technique approach was used to identify and mark genetic segments for complex quantitative traits in potatoes. Segments with a large difference in specific tags were identified in chromosome 4 and 5, and further tag validation showed that the genetic segment in chromosome 5 is associated with the early maturity trait, which is consistent with previous studies [2,3,4,8,9,10,18]. …”

Section: Discussionsupporting

confidence: 90%

“…Previous studies have shown that potato maturity was controlled by minor recessive polygenes, which were distributed in 12 chromosomes [1]; because diploid potatoes have simpler genetic ratios than tetraploids, it is easy for genetic analysis and molecular operations to be carried out. Thus, the development of potato markers and genetic map construction are mainly performed at diploid level, using traditional Quantitative Trait Loci (QTL) mapping, and most of the studies mapped the major QTL for maturity in chromosome 5, linked to the QTL for resistance to late blight [2,3,4,5,6]. In 2007, a diploid potato population was used to map a QTL associated with growth stages that is closely linked to marker BA47f2t7 (P1) in chromosome 5 [1].…”

Section: Introductionmentioning

confidence: 99%

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Mapping and QTL Analysis of Early-Maturity Traits in Tetraploid Potato (Solanum tuberosum L.)

Duan

et al. 2018

IJMS

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Early maturity is one of the most important agronomical traits in potato breeding. To identify the DNA segment that codes for early maturity, a tetraploid potato segregation population of “Zhongshu 19” × “Zhongshu 3” was genetically analyzed, using a combination of high throughput simplified genome sequencing (2b-RAD) and bulked segregant analysis (BSA). The DNA segment related to the early-maturity trait was identified at the 3.7~4.2 Mb locus on the short arm of chromosome 5. Eight molecular markers were developed, of which five were closely linked to the early-maturity trait loci. Additionally, 42 simple sequence repeats (SSR) markers were constructed based on the reference sequence of Solanum tuberosum group Phureja DM1-3 516 R44 (DM). Using the TetraploidMap software, the linkage map of chromosome 5 was constructed with 50 markers. The total map length was 172 centiMorgan (cM), with an average genetic distance of 3.44 cM. Correlating molecular and phenotypic data of the segregating population, the mapped Quantitative Trait Loci (QTL) on the short arm of chromosome 5 contributed to 33.55% of the early-maturity phenotype. The early-maturity QTL was located at 84 cM, flanked by the SSR5-85-1 and SCAR5-8 markers. The QTL was fine-mapped to 471 kb. Using DNA sequence annotation, 34 genes were identified in this region, 12 of them with unknown function. Among the other 22 annotated genes, E3 ubiquitin ligase gene PUB14 could be related to maturity and regulation of tuber formation. The constructed QTL map is a useful basic tool for the cloning of early-maturity related genes in tetraploid potatoes.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Mapping and QTL Analysis of Early-Maturity Traits in Tetraploid Potato (Solanum tuberosum L.)

Duan

et al. 2018

IJMS

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“…Positions of RFLP and SSR markers (see Supplementary Table 3 for details) linked with QTL for resistance to late blight according to the literature are shown on the left. Numbers in parenthesis code for the corresponding references: (1) Leonards-Schippers et al (1994), (2) Collins et al (1999), (3) Oberhagemann et al (1999), (7) Simko et al (2006), and (8) Brouwer et al (2004). Black bars highlight the physical segments tagged by QTL linked markers.…”

Section: Resultsmentioning

confidence: 99%

Identification of Novel Associations of Candidate Genes with Resistance to Late Blight in Solanum tuberosum Group Phureja

et al. 2017

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The genetic basis of quantitative disease resistance has been studied in crops for several decades as an alternative to R gene mediated resistance. The most important disease in the potato crop is late blight, caused by the oomycete Phytophthora infestans. Quantitative disease resistance (QDR), as any other quantitative trait in plants, can be genetically mapped to understand the genetic architecture. Association mapping using DNA-based markers has been implemented in many crops to dissect quantitative traits. We used an association mapping approach with candidate genes to identify the first genes associated with quantitative resistance to late blight in Solanum tuberosum Group Phureja. Twenty-nine candidate genes were selected from a set of genes that were differentially expressed during the resistance response to late blight in tetraploid European potato cultivars. The 29 genes were amplified and sequenced in 104 accessions of S. tuberosum Group Phureja from Latin America. We identified 238 SNPs in the selected genes and tested them for association with resistance to late blight. The phenotypic data were obtained under field conditions by determining the area under disease progress curve (AUDPC) in two seasons and in two locations. Two genes were associated with QDR to late blight, a potato homolog of thylakoid lumen 15 kDa protein ( StTL15A ) and a stem 28 kDa glycoprotein ( StGP28 ). Key message : A first association mapping experiment was conducted in Solanum tuberosum Group Phureja germplasm, which identified among 29 candidates two genes associated with quantitative resistance to late blight.

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“…Interestingly tomato powdery mildew R genes Ol-4 and Ol-6 to Oidium neolycopersici (Bai et al 2005) also reside on this region. In potato, one of the cloned genes, Rpi-blb2 for resistance to Phytophthora infestans and QTLs for resistance to P. infestans and Erwinia carotovora have been localized to this region (Oberhagemann et al 1999;Zimnoch-Guzowska et al 2000). Since there is colinearity of genetic structure between tomato and potato and the positions of resistance loci within the Solanaceae are highly conserved (Tanksley et al 1992;Grube et al 2000), comparative genetics between tomato and potato has enabled the cloning of R genes in potato.…”

Section: Discussionmentioning

confidence: 99%

A new resistance gene to powdery mildew identified in Solanum neorossii has been localized on the short arm of potato chromosome 6

Park

Jones

2008

Euphytica

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A new resistance (R) gene to powdery mildew has been identiWed and characterized in a population derived from the wild potato species, Solanum neorossii under natural infection in the greenhouse. The segregation of resistance has revealed that this R gene is controlled by a single monogenic and dominant gene designated Rpm-nrs1. Analysis of the DNA sequence on an internal transcribed spacer (ITS) region of the pathogen genome suggests that the pathogen causing the powdery mildew disease is either Golovinomyces orontii or G. cichoracearum. The resistance locus was localized to the short arm of chromosome 6 where several disease R genes already identiWed in potato and tomato are known to reside. The resistance locus cosegregated in 96 progeny with three AFLP markers and one PCR marker. The sequences of the two cosegregating AFLP markers are highly homologous to Mi-1 conferring resistance to nematode, potato aphid and whiteXy and Rpi-blb2 conferring resistance to late blight. The results in this study will facilitate the cloning of this gene conferring resistance to powdery mildew.

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Cited by 144 publications

References 30 publications

Mapping and QTL Analysis of Early-Maturity Traits in Tetraploid Potato (Solanum tuberosum L.)

Mapping and QTL Analysis of Early-Maturity Traits in Tetraploid Potato (Solanum tuberosum L.)

Identification of Novel Associations of Candidate Genes with Resistance to Late Blight in Solanum tuberosum Group Phureja

A new resistance gene to powdery mildew identified in Solanum neorossii has been localized on the short arm of potato chromosome 6

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