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Šafařı́k, Ivo; Ptáčková, L.; Šafařı́ková, Mirka

doi:10.1023/a:1013742002533

Cited by 25 publications

(2 citation statements)

References 8 publications

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“…Magnetic solid phase DNA extraction (MSPE) has advantages such as direct isolation from a crude sample, compatibility with large scale purification, and easy separation of biological debris. [119,120] To extract DNA from cell lysates of two foodborne pathogens (L. monocytogenes and Escherichia coli O157:H7), Wang et al coated a thin layer of PDA on magnetic Fe 3 O 4 NPs ( Figure 7A). [91] They used the aforementioned low-pH assisted adsorption method for the capture of DNA on the PDA surface.…”

Section: Dna Extractionmentioning

confidence: 99%

Interfacing DNA and Polydopamine Nanoparticles and Its Applications

Zandieh

Hagar

Liu

2020

Part & Part Syst Charact

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and drugs, [21,22] and decrease the toxicity of biomaterials. [23] Finally, PDA can be used for sensing. PDA-containing biosensors have been used to detect various organic molecules, [24,25] biomolecules, [26,27] and metal ions. [28] Many sensing and biomedical applications of PDA have been realized through interfacing it with biomolecules such as DNA, enzymes, and antibodies. DNA has gained popularity in the last few decades as a functional polymer thanks to its high stability, low cost, and ease of synthesis. [29-34] More importantly, DNA can bind not only its complementary sequence but also a diverse range of other molecules by using DNA aptamers. [35] Catalytically active DNA sequences, known as DNAzymes, have also been discovered. [36] Combining the unique properties of PDA and DNA has produced many interesting hybrid materials. The surface of PDA is different from most inorganic and even other organic NPs, and understanding its surface interactions is critical for applications. Given the many papers on DNA-functionalized PDA which have been published, it is now a good time to summarize this topic, which was not previously reviewed. This review has two main purposes. First, it describes the interfacial interactions between DNA and PDA with a focus on methods to achieve noncovalent and covalent conjugates. Second, we review the representative applications of such conjugates. In the end, some challenges of the field are discussed, and a few future research directions are proposed. 2. Dopamine, Its Analogs, and PDA The extraordinary wet adhesion of some marine creatures, such as mussels, is caused by their secreted proteins that are rich in catecholamine groups. This has inspired scientists to develop a universal coating material that can mimic the natural wet adhesion. [37] Although dopamine is a catecholamine, PDA is not yet found naturally. PDA is a synthetic analog of melanin, and natural melanin exists in the color pigments of the skin, hair, and eyes of the human body. Melanin is produced by the aggregation and polymerization of catecholamines such as DOPA or tyrosine (Figure 1A). [38,39] To date, the polymerization pathways of dopamine are still unknown, but a few likely pathways have been proposed. [40-44] Polydopamine (PDA) is polymerized from dopamine under oxidative and basic conditions. PDA is a biocompatible material with great versatility for coating various surfaces and it can also form nanoparticles. DNA oligonucleotides are highly stable, and they can recognize a diverse range of molecules from complementary nucleic acids, proteins to small molecules and metal ions. To enhance the molecular recognition function of PDA, it is interfaced with DNA and the conjugates have achieved a wide range of applications in biomedical and analytical science. In this review, the chemistry of some catecholamines, including dopamine and PDA, is first briefly introduced and variables in the PDA synthesis are highlighted. Strategies to promote DNA adsorption on PDA are then discussed including the use of low pH and...

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Section: Dna Extractionmentioning

confidence: 99%

Interfacing DNA and Polydopamine Nanoparticles and Its Applications

Zandieh

Hagar

Liu

2020

Part & Part Syst Charact

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“…Moreover, it is proved that magnetic separation method is one of the possible ways to recycle tiny particles from the mixture of biological waste and other fouling materials with similar sizes. Furthermore, the efficiency of magnetic separation is especially suited for large-scale purifications [190][191][192], because it only needs to apply a magnet to the side of the vessel containing the sample mixture for magnetic separation of the required particles. Nargessi et al discovered that cellulose-coated magnetic particles could adsorb nucleic acid by adding salt (NaCl) and PEG [193].…”

Section: Separation Of Nucleic Acidsmentioning

confidence: 99%

Applications of magnetic materials separation in biological nanomedicine

Chen

Iqbal

et al. 2019

Electrophoresis

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As a result of their advantages for superparamagnetic properties, good biocompatibility, and high binding capacity, functionalized magnetic materials became widely popular over the past couple of decades, being applied on large scale in various processes of sample preparation for biomedicine. In this work, we perform an in‐depth review on the current progress in the field of magnetic bead separation, discussing in detail the physical basis of this process, various synthesis methods and surface modification strategies. We place special focus of attention as well on the latest applications of magnetic polymer microspheres in cell separation, protein purification, immobilized enzyme, nucleic acid separation, and extraction of bioactive compounds with low molecular weight. Existing problems are highlighted and possible trends of magnetic separation techniques for biomedicine in the future are proposed.

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Application of Magnetic Particles in Medicine and Biology

Andrä¹,

Häfeli

Hergt³

et al. 2007

Handbook of Magnetism and Advanced Magnetic Materials

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Magnetic particles (MPs) have proved to be valuable tools for manipulation of cells or biomolecules, for transportation of chemical substances or transfer of energy to defined target sites in biological systems, and for clinical diagnostics and therapeutics—both in vitro as well as in vivo . MPs receive their magnetic properties and responsiveness to magnetic fields most often from the proven biocompatible iron oxides magnetite (Fe 3 O 4 ) and maghemite (γ‐Fe 2 O 3 ). The small size of MPs allows them to pass through capillary vessels during blood circulation. In nanoparticulate form or under the influence of strong magnetic fields, some MPs can even extravasate through capillary walls into surrounding tissue and reach many of the cells in the human body. Magnetically assisted delivery of chemo‐ or radiotherapeutics to as well as the generation of heat (hyperthermia) at defined target sites in the body can thus be achieved for treatment purposes, such as tumor therapy. Furthermore, MPs can serve as site‐ and function‐specific contrast agents and thus enhance the diagnostic potential of magnetic resonance imaging (MRI). On the biotechnological side, magnetic labeling of cells and biomolecules with MPs followed by magnetic separation has been utilized for the isolation and analysis of nucleic acids and specific cells, for protein purification, for the detection of pathogenic bacteria and viruses, and for gene transfection.

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Cited by 25 publications

References 8 publications

Interfacing DNA and Polydopamine Nanoparticles and Its Applications

Interfacing DNA and Polydopamine Nanoparticles and Its Applications

Applications of magnetic materials separation in biological nanomedicine

Application of Magnetic Particles in Medicine and Biology

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