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Muralidhar, R. V.; Chirumamilla, RR; Marchant, Roger; Ramachandran, V. N.; Ward, Owen P.; Nigam, Poonam Singh Nee

doi:10.1023/a:1014417223956

Cited by 66 publications

(20 citation statements)

References 140 publications

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“…Lipases have been widely studied for their properties and utilization in many industries [71,72,73,74,75]. Pectinases have established their role in the fruit and juice industries [76].…”

Section: Enzymes With Special Characteristics In Biotechnologymentioning

confidence: 99%

Microbial Enzymes with Special Characteristics for Biotechnological Applications

2013

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This article overviews the enzymes produced by microorganisms, which have been extensively studied worldwide for their isolation, purification and characterization of their specific properties. Researchers have isolated specific microorganisms from extreme sources under extreme culture conditions, with the objective that such isolated microbes would possess the capability to bio-synthesize special enzymes. Various Bio-industries require enzymes possessing special characteristics for their applications in processing of substrates and raw materials. The microbial enzymes act as bio-catalysts to perform reactions in bio-processes in an economical and environmentally-friendly way as opposed to the use of chemical catalysts. The special characteristics of enzymes are exploited for their commercial interest and industrial applications, which include: thermotolerance, thermophilic nature, tolerance to a varied range of pH, stability of enzyme activity over a range of temperature and pH, and other harsh reaction conditions. Such enzymes have proven their utility in bio-industries such as food, leather, textiles, animal feed, and in bio-conversions and bio-remediations.

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“…Lipases have been widely studied for their properties and utilization in many industries [71,72,73,74,75]. Pectinases have established their role in the fruit and juice industries [76].…”

Section: Enzymes With Special Characteristics In Biotechnologymentioning

confidence: 99%

Microbial Enzymes with Special Characteristics for Biotechnological Applications

2013

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“…Normalmente, mediante imobilização, o pH ótimo de atuação da lipase modifica-se parcialmente para pH mais alcalino, tendo em vista que o ataque nucleofílico da serina é auxiliado pelo comportamento básico da histidina 8,20 . Em meios ácidos, estes grupos ficam protonados e o ataque nucleofílico da serina no grupo carbonílico do substrato (triglicerídeo) pode comprometer a formação do complexo acil-enzima.…”

Section: Figura 1 Influência Do Ph Na Atividade Hidrolítica Da Lipasunclassified

Determinação das propriedades catalíticas em meio aquoso e orgânico da lipase de Candida rugosa imobilizada em celulignina quimicamente modificada por carbonildiimidazol

Gomes¹,

Paula²,

Silva³

et al. 2006

Quím. Nova

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Recebido em 11/5/05; aceito em 27/10/05; publicado na web em 31/3/06 ASSESSMENT OF CATALYTIC PROPERTIES IN AQUEOUS AND ORGANIC MEDIA OF LIPASE FROM Candida rugosa IMMOBILIZED ON WOOD CELLULIGNIN ACTIVATED WITH CARBONYLDIIMIDAZOLE. Microbial lipase from Candida rugosa was immobilized by covalent binding on wood cellulignin (Eucaliptus grandis) chemically modified with carbonyldiimidazole. The immobilized system was fully evaluated in aqueous (olive oil hydrolysis) and organic (ester synthesis) media. A comparative study between free and immobilized lipase was carried out in terms of pH, temperature and thermal stability. A higher pH value (8.0) was found optimal for the immobilized lipase. The optimal reaction temperature shifted from 37 °C for the free lipase to 45 °C for the immobilized lipase. The pattern of heat stability indicated that the immobilization process tends to stabilize the enzyme. Kinetics tests at 37 °C following the hydrolysis of olive oil obeyed the Michaelis-Menten rate equation. Values for K m = 924.9 mM and V max = 198.3 U/mg were lower than for free lipase, suggesting that the affinity towards the substrate changed and the activity of the immobilized lipase decreased during the course of immobilization. The immobilized derivative was also tested in the ester synthesis from several alcohols and carboxylic acids.Keywords: cellulignin; lipase; immobilization. INTRODUÇÃOOs processos que utilizam lipases são especialmente atraentes em função das diferentes aplicações desta enzima. As lipases podem catalisar reações de hidrólise, esterificação e interesterificação, com extrema simplicidade de processo, qualidade superior do produto final e excelente rendimento 1-3 . Estas características conferem às lipases um potencial biotecnológico comparável ao das proteases e carboidrases -enzimas industrialmente utilizadas -estimulando pesquisas para otimização da sua produção, caracterização, imobilização e aplicação industrial 2,3 .Idealmente os sistemas catalisados por lipases devem ser tratados caso a caso e as generalizações devem ser praticadas com cautela 2 . Portanto, a seleção das condições adequadas na catálise enzimática em meios não convencionais deve seguir uma cuidadosa manipulação do meio-ambiente do biocatalisador, de tal forma que a produtividade do sistema seja maximizada por meio da potencialidade total da atividade enzimática 2 . Isto pode ser alcançado pela utilização de solventes apropriados 4,5 , controle do teor de água no meio reacional 6,7 e imobilização da enzima em suportes sólidos [8][9][10] . A imobilização tem efeito benéfico na estabilidade da enzima, em função das interações físicas e químicas entre o suporte e as molécu-las da enzima. A imobilização também auxilia na dispersão homogênea da enzima no meio, o que é essencial para a condução de reações enzimáticas 9,10 .As propriedades dos derivados imobilizados são influenciadas tanto pelas propriedades da enzima como pelo material do suporte 2,11 . A interação entre esses dois componentes proporciona um derivado imobilizado...

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“…The known preference of lipase PS for the reaction with R stereogenic carbon atoms was confirmed also in these kinetic resolutions. [29] Classical organic chemistry transformations allowed the structural differentiation necessary to embed these suitably functionalised building blocks into biologically active molecules, such as hydroxylated pyrrolidines and C-phenyl glycosides.…”

Section: Discussionmentioning

confidence: 99%

Oxygenated Stereotriads with Definite Absolute Configuration by Lipase‐Mediated Kinetic Resolution: De Novo Synthesis of Imino Sugars and 6‐Deoxy‐C‐glycosides

Acetti¹,

Brenna²,

Gatti³

2010

Eur J Org Chem

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Untitled

Cited by 66 publications

References 140 publications

Microbial Enzymes with Special Characteristics for Biotechnological Applications

Microbial Enzymes with Special Characteristics for Biotechnological Applications

Determinação das propriedades catalíticas em meio aquoso e orgânico da lipase de Candida rugosa imobilizada em celulignina quimicamente modificada por carbonildiimidazol

Oxygenated Stereotriads with Definite Absolute Configuration by Lipase‐Mediated Kinetic Resolution: De Novo Synthesis of Imino Sugars and 6‐Deoxy‐C‐glycosides

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