Z-Scan Analysis: a New Method to Determine the Oxidative State of Low-Density Lipoprotein and Its Association with Multiple Cardiometabolic Biomarkers

Freitas, Maria Camila Pruper de; Neto, A. M. Figueiredo; Giampaoli, Viviana; Aubin, Elisete da Conceição Quintaneiro; Barbosa, Milena Maria de Araújo Lima; Damasceno, Nágila Raquel Teixeira

doi:10.1007/s13538-015-0395-y

Cited by 5 publications

(4 citation statements)

References 35 publications

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“…Furthermore, even after effective lipid-lowering therapies, residual atherogenic lipoproteins also predict cardiovascular risk [ 10 , 11 ], and small LDL size seems more vulnerable to oxidation and atherogenicity [ 12 ]. On the other hand, larger LDL particles with a greater number of antioxidants are considered less atherogenic and these particles can be identified by means of some properties of nonlinear optics [ 13 , 14 ]. Thus, this study aimed to evaluate the effects of randomized antiplatelet and lipid-lowering strategies, commonly prescribed in subjects with ST-segment elevation myocardial infarction (STEMI), on the pattern of LDL particles assessed by complementary methods.…”

Section: Introductionmentioning

confidence: 99%

Changes in lipoproteins associated with lipid-lowering and antiplatelet strategies in patients with acute myocardial infarction

et al. 2022

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Background Despite lipid-lowering and antiplatelet therapy, the pattern of residual lipoproteins seems relevant to long-term cardiovascular outcomes. This study aims to evaluate the effects of combined therapies, commonly used in subjects with acute myocardial infarction, in the quality of low-density lipoprotein (LDL) particles. Methods Prospective, open-label trial, included patients with acute myocardial infarction. Patients were randomized to antiplatelet treatment (ticagrelor or clopidogrel) and subsequently to lipid-lowering therapy (rosuvastatin or simvastatin/ezetimibe) and were followed up for six months. Nonlinear optical properties of LDL samples were examined by Gaussian laser beam (Z-scan) to verify the oxidative state of these lipoproteins, small angle X-ray scattering (SAXS) to analyze structural changes on these particles, dynamic light scattering (DLS) to estimate the particle size distribution, ultra violet (UV)-visible spectroscopy to evaluate the absorbance at wavelength 484 nm (typical from carotenoids), and polyacrylamide gel electrophoresis (Lipoprint) to analyze the LDL subfractions. Results Simvastatin/ezetimibe with either clopidogrel or ticagrelor was associated with less oxidized LDL, and simvastatin/ezetimibe with ticagrelor to lower cholesterol content in the atherogenic subfractions of LDL, while rosuvastatin with ticagrelor was the only combination associated with increase in LDL size. Conclusions The quality of LDL particles was influenced by the antiplatelet/lipid-lowering strategy, with ticagrelor being associated with the best performance with both lipid-lowering therapies. Trial registration: NCT02428374.

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Section: Introductionmentioning

confidence: 99%

Changes in lipoproteins associated with lipid-lowering and antiplatelet strategies in patients with acute myocardial infarction

et al. 2022

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“…The Z-scan technique was used, as previously reported, to measure non-linear optical properties of LDL samples [14][15][16][17][18][19][20]30,31 . The LDL solution sample (a low absorption medium) is encapsulated between two 1.5 cm × 2.0 cm glass microslides with a 200 μm thick Teflon spacer and is illuminated by a focused Gaussian laser beam with wavelength wave of 532 nm and power of 100 mW, propagating along the z direction.…”

Section: Z-scan Techniquementioning

confidence: 99%

“…The transmitted light intensity is measured as a function of the z position of the sample. More details about Z-scan setup and data processing can be found in our previous works [14][15][16][17][18][19][20]30,31 . One can obtain the non-linear TL amplitude θ which is related to the peak-to-valley normalized transmittance as a function of the z position of the sample.…”

Section: Z-scan Techniquementioning

confidence: 99%

“…More recently, our group validated the Z-scan (ZS) technique to investigate quality properties of LDL in relation to the balance between oxidant and antioxidant substances in this particle [14][15][16][17][18] . Briefly, the physical phenomenon present in these experiments is the thermal lens (TL) formed in a solution with LDL, when it is illuminated with a Gaussian laser beam.…”

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Effects of phytosterol supplementation on lipoprotein subfractions and LDL particle quality

Machado,

Santisteban,

Martins

et al. 2024

Sci Rep

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Phytosterols are natural components of plant-based foods used as supplements because of their known cholesterol-lowering effect. However, their effects on lipoprotein subfractions and the quality of the LDL particle have not been studied in greater detail. We aimed to evaluate the effects of phytosterols supplements on lipids, lipoproteins subfractions, and on the quality of LDL. A prospective, pilot-type, open label, cross-over study, randomized 23 males in primary prevention of hypercholesterolemia to receive diet or diet plus phytosterol (2.6 g in 2 doses, with meals) for 12 weeks, when treatments were switched for another 12 weeks. Lipoprotein subfractions were analyzed by electrophoresis in polyacrylamide gel (Lipoprint System®). The Sampson equation estimated the small and dense (sd) and large and buoyant (lb) LDL subfractions from the lipid profile. Quality of LDL particle was analyzed by Z-scan and UV–vis spectroscopy. Primary outcome was the comparison of diet vs. diet plus phytosterols. Secondary outcomes assessed differences between baseline, diet and diet plus phytosterol. Non-parametric statistics were performed with p < 0.05. There was a trend to reduction on HDL-7 (p = 0.05) in diet plus phytosterol arm, with no effects on the quality of LDL particles. Heatmap showed strong correlations (ρ > 0.7) between particle size by different methods with both interventions. Diet plus phytosterol reduced TC, increased HDL-c, and reduced IDL-B, whereas diet increased HDL7, and reduced IDL-B vs. baseline (p < 0.05, for all). Phytosterol supplementation demonstrated small beneficial effects on HDL-7 subfraction, compared with diet alone, without effects on the quality of LDL particles.This trial is registered in Clinical Trials (NCT06127732) and can be accessed at https://clinicaltrials.gov.

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Structural and Nonlinear Optical Characteristics of In Vitro Glycation of Human Low-Density Lipoprotein, as a Function of Time

et al. 2018

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Z-Scan Analysis: a New Method to Determine the Oxidative State of Low-Density Lipoprotein and Its Association with Multiple Cardiometabolic Biomarkers

Abstract: How to citeComplete issue More information about this article Journal's homepage in redalyc.org Scientific Information System Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Non-profit academic project, developed under the open access initiative

Cited by 5 publications

References 35 publications

Changes in lipoproteins associated with lipid-lowering and antiplatelet strategies in patients with acute myocardial infarction

Changes in lipoproteins associated with lipid-lowering and antiplatelet strategies in patients with acute myocardial infarction

Effects of phytosterol supplementation on lipoprotein subfractions and LDL particle quality

Structural and Nonlinear Optical Characteristics of In Vitro Glycation of Human Low-Density Lipoprotein, as a Function of Time

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