YKL107W from Saccharomyces cerevisiae encodes a novel aldehyde reductase for detoxification of acetaldehyde, glycolaldehyde, and furfural

Wang, Hanyu; Li, Qian; Zhang, Zhengyue; Zhou, Chang; Ayepa, Ellen; Abrha, Getachew Tafere; Han, Xuebing; Hu, Xiangdong; Yu, Xiumei; Xiang, Quanju; Li, Xi; Gu, Yunfu; Zhao, Ke; Xie, Chengcheng; Chen, Qiang; Ma, Menggen

doi:10.1007/s00253-019-09885-x

Cited by 18 publications

(7 citation statements)

References 47 publications

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“…For HMF, genes known to encode enzymes that catalyze this reaction are ADH1, ADH6, ADH7, ALD6, ARI1 and GRE2. For furfural, these include in addition ALD4, YDR541C, YGL039W, YNL134C and the recently identified YKL107W [12][13][14][15][16][17][18][19][20][21]. All these genes have been identified by conferring higher tolerance upon overexpression.…”

Section: Introductionmentioning

confidence: 99%

A novel AST2 mutation generated upon whole-genome transformation of Saccharomyces cerevisiae confers high tolerance to 5-Hydroxymethylfurfural (HMF) and other inhibitors

et al. 2021

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Development of cell factories for conversion of lignocellulosic biomass hydrolysates into biofuels or bio-based chemicals faces major challenges, including the presence of inhibitory chemicals derived from biomass hydrolysis or pretreatment. Extensive screening of 2526 Saccharomyces cerevisiae strains and 17 non-conventional yeast species identified a Candida glabrata strain as the most 5-hydroxymethylfurfural (HMF) tolerant. Whole-genome (WG) transformation of the second-generation industrial S. cerevisiae strain MD4 with genomic DNA from C. glabrata, but not from non-tolerant strains, allowed selection of stable transformants in the presence of HMF. Transformant GVM0 showed the highest HMF tolerance for growth on plates and in small-scale fermentations. Comparison of the WG sequence of MD4 and GVM1, a diploid segregant of GVM0 with similarly high HMF tolerance, surprisingly revealed only nine non-synonymous SNPs, of which none were present in the C. glabrata genome. Reciprocal hemizygosity analysis in diploid strain GVM1 revealed AST2N406I as the only causative mutation. This novel SNP improved tolerance to HMF, furfural and other inhibitors, when introduced in different yeast genetic backgrounds and both in synthetic media and lignocellulose hydrolysates. It stimulated disappearance of HMF and furfural from the medium and enhanced in vitro furfural NADH-dependent reducing activity. The corresponding mutation present in AST1 (i.e. AST1D405I) the paralog gene of AST2, also improved inhibitor tolerance but only in combination with AST2N406I and in presence of high inhibitor concentrations. Our work provides a powerful genetic tool to improve yeast inhibitor tolerance in lignocellulosic biomass hydrolysates and other inhibitor-rich industrial media, and it has revealed for the first time a clear function for Ast2 and Ast1 in inhibitor tolerance.

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Section: Introductionmentioning

confidence: 99%

A novel AST2 mutation generated upon whole-genome transformation of Saccharomyces cerevisiae confers high tolerance to 5-Hydroxymethylfurfural (HMF) and other inhibitors

et al. 2021

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“…Of the synXI genes, only the increase in YKL107W transcription is likely to have biological relevance. This gene encodes an aldehyde reductase involved in the detoxification of toxic aldehydes (Wang et al ., 2019). An explanation for this increase in transcription is not immediately clear to us, although recoding of the CDS to incorporate PCRTags and a BstEII restriction site may be the underlying cause.…”

Section: Resultsmentioning

confidence: 99%

Synthetic yeast chromosome XI design enables extrachromosomal circular DNA formation on demand

Blount

Driessen

et al. 2022

Preprint

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We describe construction of the 660 kilobase synthetic yeast chromosome XI (synXI) and reveal how synthetic redesign of non-coding DNA elements impact the cell. To aid construction from synthesized 5 to 10 kilobase DNA fragments, we implemented CRISPR-based methods for synthetic crossovers in vivo and used these methods in an extensive process of bug discovery, redesign and chromosome repair, including for the precise removal of 200 kilobases of unexpected repeated sequence. In synXI, the underlying causes of several fitness defects were identified as modifications to non-coding DNA, including defects related to centromere function and mitochondrial activity that were subsequently corrected. As part of synthetic yeast chromosome design, loxPsym sequences for Cre-mediated recombination are inserted between most genes. Using the GAP1 locus from chromosome XI, we show here that targeted insertion of these sites can be used to create extrachromosomal circular DNA on demand, allowing direct study of the effects and propagation of these important molecules. Construction and characterization of synXI has uncovered effects of non-coding and extrachromosomal circular DNA, contributing to better understanding of these elements and informing future synthetic genome design.

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“…In M. perniciosa , phosphatidylserine decarboxylase was detected with increased accumulation in the basidiocarp phase, which can be considered a fundamental for the development of the structure (Gomes et al, 2021). In S. cerevisiae , aldehyde reductase protects the cells by detoxifying toxic aldehyde compounds that inhibit yeast growth and fermentation (Wang et al, 2019). Thus, proteins possibly involved in the metabolism process may be essential for vegetative growth of M. roreri .…”

Section: Discussionmentioning

confidence: 99%

Comparative proteome profile of ungerminated spores and mycelium of the fungus Moniliophthora roreri, causal agent of frosty pod rot disease in cacao

Zugaib

Gramacho

Mares

et al. 2023

Journal of Phytopathology

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Moniliasis caused by the fungus Moniliophthora roreri is one of the most destructive cacao diseases in tropical America. In 2021, the presence of the pathogen was confirmed in cacao plantations in Brazil. This is the first proteomic study of the M. roreri protein profile of ungerminated spores and mycelium compared by 2D SDS‐PAGE (Two‐dimensional gel electrophoresis) associated with mass spectrometry. A total of 446 spots were detected on ungerminated spores gels and 402 spots on M. roreri mycelial gels. A total of 29 proteins were identified from the ungerminated spores, 21 of them were exclusive, and 53 were identified in the mycelium, 16 of them were exclusive. Most of the identified proteins at both development stages were categorized as being involved in metabolic processes, reduction/oxidation processes, and protein synthesis and folding. The interaction networks observed expand the known interactions of these proteins, resulting in eight functional clusters for proteins identified in ungerminated spores and seven clusters for mycelial proteins. In addition, a biological model of ungerminated spores and mycelium with cell location of proteins was constructed. The results contribute to a better understanding of the metabolic mechanisms of M. roreri during dormancy and vegetative development, which can support future studies for strategies to control moniliasis.

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YKL107W from Saccharomyces cerevisiae encodes a novel aldehyde reductase for detoxification of acetaldehyde, glycolaldehyde, and furfural

Cited by 18 publications

References 47 publications

A novel AST2 mutation generated upon whole-genome transformation of Saccharomyces cerevisiae confers high tolerance to 5-Hydroxymethylfurfural (HMF) and other inhibitors

A novel AST2 mutation generated upon whole-genome transformation of Saccharomyces cerevisiae confers high tolerance to 5-Hydroxymethylfurfural (HMF) and other inhibitors

Synthetic yeast chromosome XI design enables extrachromosomal circular DNA formation on demand

Comparative proteome profile of ungerminated spores and mycelium of the fungus Moniliophthora roreri, causal agent of frosty pod rot disease in cacao

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