Yin-xing-tong-mai decoction attenuates atherosclerosis via activating PPARγ-LXRα-ABCA1/ABCG1 pathway

Zheng, Shasha; Huang, Hong; Li, Yizhuo; Wang, Ye; Zheng, Yawei; Liang, Jing; Zhang, Siqi; Liu, Ming; Fang, Zheng

doi:10.1016/j.phrs.2021.105639

Cited by 46 publications

(29 citation statements)

References 61 publications

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“…PPAR-γ upregulates the expression of ABCA1 and SR-B1 by activating downstream effector molecules. PPAR-γ also promotes the outflow of cholesterol from macrophages [37]. Liver X receptor (LXR), as a non-steroid hormone receptor, is a metabolic nuclear receptor that maintains the balance of cholesterol and lipid metabolism by regulating the expression of target genes [38].…”

Section: Discussionmentioning

confidence: 99%

Preventive Effect and Molecular Mechanism of Lactobacillus rhamnosus JL1 on Food-Borne Obesity in Mice

et al. 2021

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Probiotics can prevent obesity and related metabolic complications. In our study, the protective effect and molecular mechanism of Lactobacillus rhamnosus JL1 (separated from the feces of healthy infants) on high-fat diet mice were investigated. After 10 weeks of dietary intervention with L. rhamnosus JL1 intervention, the body weight of the JL1 group (23.78 g) was significantly lower than that of the HFD group (26.59 g, p < 0.05) and the liver index was reduced. Serum biochemical analysis showed that the TC, TG and LDL-C contents of JL1 group mice were significantly decreased (p < 0.05). Histological images of the mice livers showed that the degree of lipid action and damage of hepatic cells were improved. L. rhamnosus JL1 activated the AMPK pathway, and reduced the gene expression of PPAR-γ, LXR-α and SREBP-1C. In addition, the protein expression of PPAR-γ and LXR-α were reduced. After dietary intervention with L. rhamnosus JL1, the concentration of acetic acid, propionic acid, and butyric acid were increased significantly, especially the concentration of butyric acid, which was 63.16% higher than that of the HFD group (p < 0.05). In conclusion, this study provided a theoretical reference for the development and application of probiotics derived from healthy infant feces in health products and functional foods.

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Section: Discussionmentioning

confidence: 99%

Preventive Effect and Molecular Mechanism of Lactobacillus rhamnosus JL1 on Food-Borne Obesity in Mice

et al. 2021

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“…Botanical drugs are gaining increasing acceptance worldwide, for example, using Yin-xin-tong-mai, Huang-qi san, or Danning tablets. These TCMs have been shown to positively affect obesity ( Hao et al, 2020 ; Ma et al, 2021 ; Zheng et al, 2021 ). The present study results also confirmed that MMQ-8 has positive effects on obesity.…”

Section: Discussionmentioning

confidence: 99%

“…These botanical drugs are rich in terpenoids, flavonoids, alkaloids, phenolic acids, fatty acids, and other substances that have multiple pharmacological effects. Baicalein, boldine, scutellarein, quercetin, and conjugated linoleic acid are all known to optimize lipid metabolism and decrease inflammation by regulating NF-κB, peroxisome proliferator-activated receptor (PPAR), and the phosphatidylinositol 3-kinase (PI3K) signaling pathway ( Yang et al, 2018 ; den Hartigh, 2019 ; Lin et al, 2019 ; Yang et al, 2019 ; Dai et al, 2021 ). Therefore, we believe that MMQ-8 has a regulatory effect on lipid metabolism and inflammation.…”

Section: Introductionmentioning

confidence: 99%

The Traditional Mongolian Medicine Qiqirigan-8 Effects on Lipid Metabolism and Inflammation in Obesity: Pharmacodynamic Evaluation and Relevant Metabolites

Narenmandula

Hongmei²,

Ding³

et al. 2022

Front. Pharmacol.

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Objective: Traditional Mongolian Medicine Qiqirigan-8 (MMQ-8) is a Chinese botanical drug with effective pharmacological properties in obesity. However, the pharmacological mechanism of MMQ-8 remains unclear. This study aimed to determine the active metabolites of MMQ-8 and its therapeutic effects on lipid metabolism and inflammation.Methods: The active metabolites of MMQ-8 were identified by ultrahigh-performance liquid chromatograph Q extractive mass spectrometry (UHPLC-QE-MS) assay and network analysis. An obesity rat model induced by high-fat diet was used in the study. Serum levels of lipids and inflammatory factors were detected using biochemical analysis and enzyme-linked immunosorbent assay (ELISA). Pathological analysis of liver tissues and arteries was conducted with hematoxylin and eosin (H&E) staining and immunohistochemistry. Protein expression of the tumor necrosis factor (TNF) signaling pathway was investigated by Western-blot. Simultaneously, bone marrow cells were used for RNA sequencing and relevant results were validated by cell culture and quantitative real-time polymerase chain reaction (RT-qPCR).Results: We identified 69 active metabolites and 551 target genes of MMQ-8. Of these, there are 65 active metabolites and 225 target genes closely related to obesity and inflammation. In vivo, we observed that MMQ-8 had general decreasing effects on body weight, white adipose tissue weight, and serum lipids. MMQ-8 treatment notably decreased the liver function markers and hepatic steatosis, and significantly decreased inflammation. In serum, it notably decreased TNF-α, interleukin (IL)-6, and inducible nitric oxide synthase (INOS), while elevating IL-10 levels. MMQ-8 treatment also significantly inhibited proteins phosphorylation of nuclear factor-kappa B inhibitor alpha (IκBα), mitogen-activated protein kinase (p38), extracellular regulated kinase 1/2(ERK1/2), and stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK), and decreased vascular endothelium damage and macrophage infiltration and polarization to M1. These findings coincide with the RNA-sequencing data of bone marrow cells and results of in vitro experiments.Conclusion: We determined the pharmacological actions and relevant metabolites of MMQ-8 in obesity for the first time. Our study revealed MMQ-8 can optimize lipid metabolism and reduce chronic inflammation in obesity. However, more in-depth research is needed, for example, to understand the principle of compound compatibility and the inhibition effects on hepatic steatosis, T cell differentiation, and inflammatory signal transduction.

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“…Interestingly, many traditional Chinese medicines are involved in ABCA1 regulation and demonstrate efficacy against atherosclerosis. Yin-xing-tong-mai and Sini decoctions [ 193 ] increase ABCA1 expression in macrophages by activating the PPARγ–LXRα pathway to attenuate atherosclerosis [ 194 ]. The Qing-Xue-Xiao-Zhi formula inhibits the TLR4/MyD88/NF-κB pathway to promote ABCA1 expression [ 195 ].…”

Section: Abca1 and Cardiovascular Diseasesmentioning

confidence: 99%

Role of ABCA1 in Cardiovascular Disease

Wang

Xiao

Wang

et al. 2022

JPM

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Cholesterol homeostasis plays a significant role in cardiovascular disease. Previous studies have indicated that ATP-binding cassette transporter A1 (ABCA1) is one of the most important proteins that maintains cholesterol homeostasis. ABCA1 mediates nascent high-density lipoprotein biogenesis. Upon binding with apolipoprotein A-I, ABCA1 facilitates the efflux of excess intracellular cholesterol and phospholipids and controls the rate-limiting step of reverse cholesterol transport. In addition, ABCA1 interacts with the apolipoprotein receptor and suppresses inflammation through a series of signaling pathways. Thus, ABCA1 may prevent cardiovascular disease by inhibiting inflammation and maintaining lipid homeostasis. Several studies have indicated that post-transcriptional modifications play a critical role in the regulation of ABCA1 transportation and plasma membrane localization, which affects its biological function. Meanwhile, carriers of the loss-of-function ABCA1 gene are often accompanied by decreased expression of ABCA1 and an increased risk of cardiovascular diseases. We summarized the ABCA1 transcription regulation mechanism, mutations, post-translational modifications, and their roles in the development of dyslipidemia, atherosclerosis, ischemia/reperfusion, myocardial infarction, and coronary heart disease.

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Yin-xing-tong-mai decoction attenuates atherosclerosis via activating PPARγ-LXRα-ABCA1/ABCG1 pathway

Cited by 46 publications

References 61 publications

Preventive Effect and Molecular Mechanism of Lactobacillus rhamnosus JL1 on Food-Borne Obesity in Mice

Preventive Effect and Molecular Mechanism of Lactobacillus rhamnosus JL1 on Food-Borne Obesity in Mice

The Traditional Mongolian Medicine Qiqirigan-8 Effects on Lipid Metabolism and Inflammation in Obesity: Pharmacodynamic Evaluation and Relevant Metabolites

Role of ABCA1 in Cardiovascular Disease

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