Seed of a Mexican semidwarf wheat (Triticum aestivum L. cv. Inia 66), was obtained from a nitrogen fertilizer field trial grown in Mexico. A high positive correlation was obtained between seed protein content and seedling dry weight after 3 weeks growth (r = +0.92**). The seedling dry weight was positively related to the protein content of the aleurone layer and endosperm, but not to the embryo. Small, 35 Studies have shown that seed source, size, and protein content of both monocotyledonous and dicotyledonous species are positively related to early seedling growth and sometimes to economic yield (5, 9, 10, 14-16). In wheat, seed size affects seedling vigor, particularly when plants are grown under nonstressing conditions. Some field studies using high plant densities have shown similar results (3).McFadden (13) suggested that the chemical composition of seed can be an important factor in determining seedling vigor when seeds of the same cultivar are obtained from different sources. Other studies have shown high positive correlations between both seed protein and its constituent amino acids, and seedling dry weight during the first 3 weeks of development (11,12). Factors other than protein content of the seed have also been shown to be related to seedling vigor (1, 2). However, this research was conducted with aging seed. As intact cereal grains age they lose their ability to metabolize glucose into CO2, polysaccarides, and proteins (1). Anderson and Abdul-Baki (2) showed that changes in respiration and glucose utilization in embryos and endosperms do not proceed at the same rate nor go in the same direction during deterioration. The objective of this study was to determine which part of the wheat seed is responsible for the positive relationship between seed protein and seedling growth by using seed containing different quantities of protein due to nitrogen applications in the field.
MATERIALS AND METHODSStandardization of Seed Material. Seed of a Mexican semidwarf wheat (Triticum aestivum L. cv. Inia 66) was obtained from a fertilizer trial conducted in Mexico during the 1970 season. Seed from each of the seven samples, from plots receiving fertilizer applications in the range of 0 to 300 kg N/ha2, were individually weighed to 40 ± 2 mg, milled, and assayed for total protein by an automated Kjeldahl procedure (7). Ten seeds from each fertilizer treatment were sown in plastic pots containing vermiculite for growth analysis. After 10 days, stands were thinned to six seedlings per pot; at the same time 100 ml of a one-half strength Hoagland's nutrient solution (8) containing 3 mi NO3-was applied. The seedlings were grown for 21 days at 25/15 C with 16-hr daylength and a light intensity of 13.5, 9.0, 3.6 ,uw/cm2-nm in the blue, red, and far red ranges respectively. Dry weights were determined on root and shoot material.Protein Determinations on Seed Parts. Forty seeds (40 + 2 mg) from each of the seven plots were placed in a humid atmosphere for 48 hr and then dissected into embryo, endosperm, and aleuro...