YHV-protease dsRNA inhibits YHV replication in Penaeus monodon and prevents mortality

Yodmuang, Supansa; Tirasophon, Witoon; Roshorm, Yaowaluck Maprang; Chinnirunvong, Wanlop; Panyim, Sakol

doi:10.1016/j.bbrc.2005.12.186

Cited by 182 publications

(123 citation statements)

References 21 publications

Supporting

Mentioning

117

Contrasting

Order By: Relevance

“…One observation that has occurred a few times in the literature is the fact that non-target, non-specific dsRNA used as negative controls in viral knockdown experiments has had a protective effect against some viruses including WSSV [154], YHV [207] and even totally unrelated, nonspecific dsRNA (green fluorescent protein) down regulated YHV genes in Penaeus monodon [207]. Similarly, La Fauce and Owens [93] found non-specific dsRNA knocked down against PmergDNV (see above).…”

Section: Non-specific Protective Effects Of Dsrnasmentioning

confidence: 92%

“…Most authors have delivered the iRNA 24 h before challenge with the virus with satisfactory results. Even up to 4 days but not 5 days administration prior to exposure was protective against YHV [207]. Only one study has shown that delivery of dsRNA 24 h after exposure to YHV was acceptable but 48 h after exposure was too long [143].…”

Section: Dose and Timing Of Prophylactic Delivery Of Rnaimentioning

confidence: 99%

“…Early studies showed down regulation of genes based on the presence/absence or lighter amplicons on gels [112,155,207]. When the titre was measured by quantitative PCR, the viral titre was reduced twofold to 1 log reduction.…”

Section: Effects On Viral Titrementioning

confidence: 99%

See 2 more Smart Citations

RNA Interference with Special Reference to Combating Viruses of Crustacea

Fauce

Owens

2012

Indian J. Virol.

View full text Add to dashboard Cite

RNA interference has evolved from being a nuisance biological phenomenon to a valuable research tool to determine gene function and as a therapeutic agent. Since pioneering observations regarding RNA interference were first reported in the 1990s from the nematode worm, plants and Drosophila, the RNAi phenomenon has since been reported in all eukaryotic organisms investigated from protozoans, plants, arthropods, fish and mammals. The design of RNAi therapeutics has progressed rapidly to designing dsRNA that can specifically and effectively silence disease related genes. Such technology has demonstrated the effective use of short interfering as therapeutics. In the absence of a B cell lineage in arthropods, and hence no long term vaccination strategy being available, the introduction of using RNA interference in crustacea may serve as an effective control and preventative measure for viral diseases for application in aquaculture.

show abstract

Section: Non-specific Protective Effects Of Dsrnasmentioning

confidence: 92%

Section: Dose and Timing Of Prophylactic Delivery Of Rnaimentioning

confidence: 99%

See 1 more Smart Citation

RNA Interference with Special Reference to Combating Viruses of Crustacea

Fauce

Owens

2012

Indian J. Virol.

View full text Add to dashboard Cite

show abstract

“…The recombinant plasmids were transformed into the E. coli strain HT115, and dsRNAs were purified as described by Yodmuang et al (37). dsRNA was microinjected into Artemia using the Ultra-MicroPump II equipped with a Micro4 TM MicroSyringe pump controller.…”

Section: Methodsmentioning

confidence: 99%

Two p90 Ribosomal S6 Kinase Isoforms Are Involved in the Regulation of Mitotic and Meiotic Arrest in Artemia

Duan

Zhang

Chen

et al. 2014

Journal of Biological Chemistry

View full text Add to dashboard Cite

Background: Ribosomal S6 kinase (RSK) plays important roles in meiosis and mitosis. Results: Ar-Rsk1 promoted cdc2 phosphorylation and thereby meiotic arrest, whereas Ar-Rsk2 knockdown resulted in mitotic arrest. Conclusion: Ar-Rsk1, which lacks an ERK-docking motif, controls meiotic arrest, whereas Ar-Rsk2, which has an ERK-docking motif, controls mitotic arrest. Significance: The data provide insight into the functions of RSK isoforms.

show abstract

“…The GFP fragment was then subcloned into pET-T7 in a similar manner as ArBAP1. Recombinant plasmids were transformed into E. coli HT115, and dsRNAs were purified as described by Yodmuang et al (2006). A dose gradient (20, 40, 60, 80, and100 ng) of ArBAP1 dsRNA and 100 ng of control GFP dsRNA were injected into the body cavity of Artemia just before ovarian development, with 100 individuals injected for each preparation.…”

Section: In Vitro Enzyme Activity Assaysmentioning

confidence: 99%

Deubiquitinating enzyme BAP1 is involved in the formation and maintenance of the diapause embryos of Artemia

Yang

Jia

et al. 2012

Cell Stress and Chaperones

View full text Add to dashboard Cite

The modification of proteins by ubiquitination and deubiquitination plays an important role in various cellular processes. BRCA1-associated protein-1 (BAP1) is a deubiquitinating enzyme whose function in the control of the cell cycle requires both its deubiquitinating activity and nuclear localization. In the present study, a ubiquitin carboxylterminal hydrolase belonging to the BAP1 family was identified and characterized from Artemia parthenogenetica, a member of a family of brine shrimp that, under certain conditions, produce and release diapause embryos in which cell division and turnover of macromolecules are arrested. Western blot analysis and in vitro enzyme activity assay revealed ArBAP1 to be a cytoplasmic protein with typical ubiquitin hydrolase activity. Northern blot analysis revealed that ArBAP1 was abundant in the abdomen of Artemia producing diapause-destined embryos. Furthermore, by in situ hybridization, ArBAP1 was located exclusively in the embryos. In vivo knockdown of ArBAP1 by RNA interference resulted in the formation of embryos with split shells and abortive nauplii. The present findings suggest that ArBAP1, the first reported cytoplasmic BAP1, participates in the formation of diapause embryos and plays an important role in the control of cell cycle arrest in these encysted embryos.

show abstract

YHV-protease dsRNA inhibits YHV replication in Penaeus monodon and prevents mortality

Cited by 182 publications

References 21 publications

RNA Interference with Special Reference to Combating Viruses of Crustacea

RNA Interference with Special Reference to Combating Viruses of Crustacea

Two p90 Ribosomal S6 Kinase Isoforms Are Involved in the Regulation of Mitotic and Meiotic Arrest in Artemia

Deubiquitinating enzyme BAP1 is involved in the formation and maintenance of the diapause embryos of Artemia

Contact Info

Product

Resources

About