Yersinia enterocolitica prevalence, on fresh pork, poultry and beef meat at retail level, in France

Esnault, Emilie; Labbé, A.; Houdayer, Catherine; Denis, M.

doi:10.31274/safepork-180809-914

Cited by 4 publications

(6 citation statements)

References 9 publications

(15 reference statements)

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“…Our findings agreed with those of other researchers that biotype 1A is the most prevalent, or even the only isolated biotype, in poultry and meat [ 16 , 23 , 26 – 28 ]. It must be noted that some studies report that strains of biotype 1A can occasionally act as opportunistic pathogens and cause extraintestinal infections [ 29 ].…”

Section: Discussionsupporting

confidence: 93%

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Detection ofYersinia enterocoliticain Retail Chicken Meat, Mashhad, Iran

Sirghani

Zeinali

Jamshidi

2018

Journal of Pathogens

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Poultry meat is one of the most important sources of infection of Yersinia spp. for humans. The aim of the present study was to evaluate the incidence of Yersinia enterocolitica in chicken meat by using culture method on selective medium and confirmation by PCR assay. Also, biochemical methods were used for biotyping. A total of 100 chicken thigh meat samples were collected randomly from retail outlets in Mashhad, Iran. Samples were enriched in Peptone-Sorbitol-Bile (PSB) broth and then cultured on Cefsulodin-Irgasan-Novobiocin (CIN) agar containing antibiotics supplement. The DNA was extracted from suspected colonies of Yersinia spp. and then PCR test using specific primers for 16S rRNA gene of Yersinia enterocolitica was performed. In this study, 30% of chicken meat was contaminated with Yersinia spp. by culture method and 25% of chicken meat was contaminated with Yersinia enterocolitica. Biotyping of isolated colonies showed that all of the isolates belonged to biotype 1A. Culture and detection of Yersinia spp. from food samples traditionally take 4 days. Due to high accuracy and speed of PCR assay, it is a good alternative method for microbiological techniques. In conclusion, poultry meat can act as a source of Y. enterocolitica and could be considered as a public health hazard.

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Section: Discussionsupporting

confidence: 93%

“…enterocolitica-positive chicken eggshell samples (38.65%) if compared to our results [ 22 ]. In France, 5.2% of poultry were contaminated with Y. Enterocolitica and all of the isolates were detected as biotype 1A [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

Detection ofYersinia enterocoliticain Retail Chicken Meat, Mashhad, Iran

Sirghani

Zeinali

Jamshidi

2018

Journal of Pathogens

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“…The variation in prevalence between these studies was mainly because of differences in sample size, meat matrices tested, and geographical locations. Compared to the current study, a lower prevalence has been reported in Egypt at 6% (Younis, Elkenany & Dowidar 2021 ), France at 5% (Esnault et al 2018 ), and India at 1% (Latha et al 2017 ). The high prevalence in the current study is very alarming and poses a potential serious public health hazard in the country.…”

Section: Discussioncontrasting

confidence: 87%

Characterisation and antibiotic resistance of Yersinia enterocolitica from various meat categories, South Africa

Seakamela

Diseko²,

Malatji

et al. 2022

Onderstepoort j. vet. res.

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Yersinia enterocolitica infections impose a significant public health and socioeconomic burden on human population in many countries. The current study investigated the prevalence, antimicrobial resistance profile and molecular diversity of Y. enterocolitica in meat and meat products across various retail outlets in selected provinces of South Africa (SA). In a cross-sectional study, a total of 581 retail meat and meat products were collected from four cities across three provinces of SA. Samples were from beef and pork products, which included 292 raw intact, 167 raw processed, and 122 ready-to-eat (RTE) meats. Samples were analysed using classical microbiological methods for isolation, identification and biotyping of Y. enterocolitica. Conventional polymerase chain reaction (PCR) was performed for confirmation, serotyping, screening of virulence (n = 11) and antimicrobial resistance (n = 18) genes. Phenotypic antimicrobial resistance profiles were determined against 12 antibiotics discs, using disc diffusion method. The overall prevalence of 12% (70/581) was reported across all cities with contamination proportion reported in samples collected from raw intact 15% (43/292), followed by raw processed 11% (18/167) and RTE meats 7% (9/122). All positive isolates were of biotype 1A with 7% (5/70) belonging to bioserotype 1A/O:8. Most of the isolates harboured ymoA, ystB, fepD, ail, fepA, invA and myfA virulence genes. High antimicrobial resistance frequency was observed for ampicillin (94%), cephalothin (83%) and amoxicillin (41%), respectively. Of the 18 tested antimicrobial resistance genes, blaTEM was the most predominant (40%) followed by cmlA (21%). This study reveals the presence of antimicrobial resistant Y. enterocolitica possessing virulent genes of public health importance in products of animal origin, therefore, health monitoring and surveillance of this pathogen is required.

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“…According to Esnault et al . [ 23 ], 5.2% beef samples were contaminated with Y. enterocolitica while in this study, 0.57% samples were found positive. According to Liang et al .…”

Section: Discussionmentioning

confidence: 48%

Prevalence of Listeria monocytogenes, Yersinia enterocolitica, Staphylococcus aureus, and Salmonella enterica Typhimurium in meat and meat products using multiplex polymerase chain reaction

Latha¹,

Anu²,

Ajaykumar³

et al. 2017

Vet World

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Aim:The objective of the study was to investigate the occurrence of Listeria monocytogenes, Yersinia enterocolitica, Staphylococcus aureus, and Salmonella enterica Typhimurium in meat and meat products using the multiplex polymerase chain reaction (PCR) method.Materials and Methods:The assay combined an enrichment step in tryptic soy broth with yeast extract formulated for the simultaneous growth of target pathogens, DNA isolation and multiplex PCR. A total of 1134 samples including beef (n=349), chicken (n=325), pork (n=310), chevon (n=50), and meat products (n=100) were collected from different parts of Kerala, India. All the samples were subjected to multiplex PCR analysis and culture-based detection for the four pathogens in parallel.Results:Overall occurrence of L. monocytogenes was 0.08 % by cultural method. However, no L. monocytogenes was obtained by multiplex PCR method. Yersinia enterocolitica was obtained from beef and pork samples. A high prevalence of S. aureus (46.7%) was found in all types of meat samples tested. None of the samples was positive for S. Typhimurium.Conclusion:Multiplex PCR assay used in this study can detect more than one pathogen simultaneously by amplifying more than one target gene in a single reaction, which can save time and labor cost.

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Yersinia enterocolitica prevalence, on fresh pork, poultry and beef meat at retail level, in France

Cited by 4 publications

References 9 publications

Detection ofYersinia enterocoliticain Retail Chicken Meat, Mashhad, Iran

Detection ofYersinia enterocoliticain Retail Chicken Meat, Mashhad, Iran

Characterisation and antibiotic resistance of Yersinia enterocolitica from various meat categories, South Africa

Prevalence of Listeria monocytogenes, Yersinia enterocolitica, Staphylococcus aureus, and Salmonella enterica Typhimurium in meat and meat products using multiplex polymerase chain reaction

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