Yersinia enterocolitica infection in children

Abdel‐Haq, Nahed; Asmar, Basim I.; Abuhammour, Walid; Brown, William J.

doi:10.1097/00006454-200010000-00002

Cited by 123 publications

(35 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In some cases, septicemia can occur, depending on the health of the host. The most serious illnesses are developed by young children under the age of 1 year (1). Y. enterocolitica is one of three humanpathogenic species in the genus Yersinia that, along with Yersinia pseudotuberculosis, causes gastroenteritis.…”

mentioning

confidence: 99%

Effect of Flagellar Mutations on Yersinia enterocolitica Biofilm Formation

Kim

Young

2008

Appl Environ Microbiol

View full text Add to dashboard Cite

Yersinia enterocolitica biovar 1B is one of a number of strains pathogenic to humans in the genus Yersinia. It has three different type III secretion systems, Ysc, Ysa, and the flagella. In this study, the effect of flagella on biofilm formation was evaluated. In a panel of 31 mutant Y. enterocolitica strains, we observed that mutations that abolish the structure or rotation of the flagella greatly reduce biofilm formation when the bacteria are grown under static conditions. These results were further evaluated by assessing biofilm formation under continuous culture using a flow cell chamber. The results confirmed the important contribution of flagella to the initiation of biofilm production but indicated that there are differences in the progression of biofilm development between static growth and flow conditions. Our results suggest that flagella play a critical role in biofilm formation in Y. enterocolitica.Yersinia enterocolitica is a food-borne, pathogenic, gramnegative bacterium that causes gastroenteritis. In some cases, septicemia can occur, depending on the health of the host. The most serious illnesses are developed by young children under the age of 1 year (1). Y. enterocolitica is one of three humanpathogenic species in the genus Yersinia that, along with Yersinia pseudotuberculosis, causes gastroenteritis. The third species, Yersinia pestis, is the causative agent of plague. Historically, the formation of biofilms by Y. pestis has been better studied than biofilms of the other two. It has been suggested that Y. pestis biofilms induce starvation of fleas by blocking their digestive tracts. These biofilms seem to affect the fleas but play no role in mammalian pathogenesis, as Y. pestis strains unable to form biofilms are still able to be transmitted by fleas and cause disease (23-25, 53).There are many studies showing the importance of flagella in biofilm formation in other bacteria (32,61,67). In Escherichia coli, a screen showed that 34 of 72 adhesion-deficient strains had changes in motility characteristics (31). Another screen showed that about half the strains that were biofilm defective also exhibited changes in motility function (67), and while motility was a critical factor under certain biofilm formation conditions, chemotaxis was not required (67). Flagella are involved in the adherence of enteropathogenic E. coli to epithelial cells (32), and flagella are also important for biofilm development in Pseudomonas aeruginosa and Aeromonas spp. (61). However, there are other studies that suggest that flagella are not essential for biofilm formation in E. coli (68), P. aeruginosa (47), and Pseudomonas fluorescens (74). These studies suggest that the critical contribution of flagella to biofilm formation is conditional.Among the three pathogenic Yersinia strains, only Y. enterocolitica and Y. pseudotuberculosis are motile. Y. pestis has a frameshift in flhD, the flagellar-biosynthesis regulator protein, and is therefore nonmotile. The goal of this study was to determine whether flagella and motility...

show abstract

mentioning

confidence: 99%

Effect of Flagellar Mutations on Yersinia enterocolitica Biofilm Formation

Kim

Young

2008

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…For example, Yersinia enterocolitica is a gram-negative enteric pathogen that causes gastroenteritis, inflammation of the mesenteric lymph nodes (MLN), and in some rare cases, septicemia (10,43). Study of this pathogen is particularly relevant since it is considered a prevalent and emerging cause of childhood gastrointestinal disease in the United States (1,33,40). Indeed, it has been reported that two-thirds of Y. enterocolitica infections occur among infants and children (9).…”

mentioning

confidence: 99%

Murine Neonates Are Highly Resistant toYersinia enterocoliticafollowing Orogastric Exposure

2007

View full text Add to dashboard Cite

Neonates are considered highly susceptible to gastrointestinal infections. This susceptibility has been attributed partially to immaturity in immune cell function. To study this phenomenon, we have developed a model system with murine neonates, using the natural orogastric route of transmission for the enteropathogen Yersinia enterocolitica. The susceptibilities of 7-day-old and adult mice to orogastric Y. enterocolitica infection were assessed in 50% lethal dose experiments. Remarkably, neonatal mice of either the BALB/c or C57BL/6 mouse strain showed markedly enhanced survival after infection compared to adult mice. The resistance of neonates was not due to failure of the bacteria to colonize neonatal tissues; Y. enterocolitica was readily detectable in the intestine and mesenteric lymph nodes (MLN) for at least 1 week after infection. In adult mice, Y. enterocolitica rapidly disseminated to the spleen and liver. In striking contrast, bacterial invasion of the spleen and liver in neonates was limited. Using flow cytometry and histology, we found substantial increases in the percentages of neutrophils and macrophages in the neonatal MLN, while influx of these cells into the adult MLN was limited. Similar results were obtained using two different high-virulence Y. enterocolitica strains. Importantly, depletion of neutrophils with a specific antibody led to increased translocation of the bacteria to the spleens and livers of neonates. Together, these experiments support the hypothesis that the neonatal intestinal immune system can rapidly mobilize innate phagocytes and thereby confine the bacterial infection to the gut, resulting in a high level of resistance.

show abstract

“…Salmonella and Shigella species, Escherichia coli (Pickering, 2004), Listeria monocytoglues (Ooi & Lorber, 2005), Yersinia enterocolytica (Abel-Haq et al, 2000) are the pathogens involved with this disease. The use of antimicrobial agents for treatment of the diarrhea is limited because of the resistance among enteric pathogens.…”

Section: Resultsmentioning

confidence: 99%

Biological activity of Herissantia crispa (L.) Brizicky

Lima¹,

Costa²,

Matias³

et al. 2009

Rev. bras. farmacogn.

View full text Add to dashboard Cite

RESUMO: "Atividade biológica da Herissantia crispa (L.) Brizicky". O extrato metanólico bruto (EMeOH) das partes aéreas da Herissantia crispa (L.) Brizicky, planta rica em flavonóides e que não possui estudos farmacológicos, foi testada para avaliar sua atividade sob os parâmetros comportamentais e determinar a dose letal 50 (DL 50 ) em camundongos; atividade antimicrobiana e antiulcerogênica. O EMeOH (5000 mg/kg, v.o. ou 2000 mg/kg i.p.) não alterou os parâmetros comportamentais e não causou mortes nos animais. A amostra vegetal em estudo inibiu o crescimento bacteriano. O EMeOH (750 mg/kg) apresentou atividade anti-diarréica. O EMeOH (250, 500 e 750 mg/kg) foi capaz de inibir as lesões gástricas induzidas pelo 0,3 M HCl/etanol 60% em camundongos. Desta forma, pode-se concluir que a planta em estudo apresenta atividade antiulcerogênica, entretanto, se faz necessário avaliar tal atividade através de modelos mais específicos e estudar o mecanismo de ação pelo qual a amostra vegetal protege a mucosa gástrica.Unitermos: Herissantia crispa, Malvaecae, avaliação comportamental, atividade antibacteriana, atividade antiulcerogênica. ABSTRACT:The crude methanol extract (EMeOH) of the aerial parts of Herissantia crispa (L.) Brizicky, plant riches in flavonoids and without pharmacological studies, was tested to value its activity under the behaviour parameters and to determine the lethal dose (LD 50 ) in mice; antimicrobial and antiulcerogenic activities. The EMeOH (5,000 mg/kg, v.o. or 2,000 mg/kg i.p.) did not alter the behaviour parameters and there were not mice deaths. The extract inhibited the bacterial growth. The EMeOH (750 mg/kg) showed anti-diarroeal activity. The EMeOH (250, 500 and 750 mg/kg) decreased the gastric lesions induced by 0.3 M HCl/ethanol 60% in mice. In conclusion, the EMeOH presents anti-ulcerogenic activity;, however it is necessary to value the antiulcerogenic activity in more specific models and to study the action mechanism by which the vegetable sample protects the gastric mucosa.

show abstract

Yersinia enterocolitica infection in children

Cited by 123 publications

References 28 publications

Effect of Flagellar Mutations on Yersinia enterocolitica Biofilm Formation

Effect of Flagellar Mutations on Yersinia enterocolitica Biofilm Formation

Murine Neonates Are Highly Resistant toYersinia enterocoliticafollowing Orogastric Exposure

Biological activity of Herissantia crispa (L.) Brizicky

Contact Info

Product

Resources

About