Yerli mandarin x Carrizo sitranjı melezlerinde, embriyo kurtarma tekniğinde embriyo gelişim aşaması ve GA3 dozlarının optimizasyonu

Abstract

Many difficulties are exist in citrus breeding due to incompatibility, long juvenility, sterility and nucellar embryo. Zygotic embryo in the seeds of polyembryonic citrus cultivar is degenerated by nucellar embryos during embryo development. Accordingly, in vitro embryo culture is a useful tool in citrus breeding, since it assures embryo germination and development. In this study, Common mandarin x Carrizo citrange were crossed in order to produce new rootstock genotypes. Immature embryos were taken from fruit after 80, 100 and 120 pollination days to determine the suitable embryo rescue stage. Then, the embryos were germinated in Murashige and Tucker (MT) culture medium including 0, 0.5 and 1 mg l-1 GA3. According to the results, high germination rates were materialized as 100% and on 95% to taken embryos from 120 days after pollination (DAP) in supplemented with 1 mg l-1 GA3 and 0.5 mg l-1 GA3 in MT culture medium respectively. The embryos taken from 80 days after pollination did not germinate on MT medium without GA3 (control) and including with 0.5 mg l-1. Generally, the existence of GA3 on medium increased the ratio of germination compared to the control. The highest trifoliate ratio was determined as 33.33% at the embryos taken from 100 and 120 days after pollination and medium including 0.5 and 1 mg l-1 GA3 consecutively. The results have revealed that the optimal time for embryo rescue at citrus is 120 DAP and including GA3 in the medium increased embryo germination.

Optimization of Kinetin Concentrations and Medium Compositions for Citrus Shoot Multiplication From Cotiledonary Node

“…Several studies have shown that Murashige and Skoog (MS) media modified by Morel & Wetmore vitamins (VMW) are suitable for in vitro growth of citrus, as reported in somatic embryogenesis of mandarin (Purwito et al 2015), somatic embryogenesis from endosperm tissue of C. nobilis (Kosmiatin et al 2014), and in micropropagation and micrografting of Japansche Citroen (Putra et al 2015). Conversely, Murashige & Tucker (MT) medium is also widely utilized in citrus plants, including in vitro propagation of Thomson navel sweet oranges (Esmaeilnia & Dehestani 2015) and embryo rescue in progenies resulting from crosses between mandarin and Carrizo citrange (Kurt & Ulger 2019).…”