Yeasts isolated from figs (Ficus carica L.) as biocontrol agents of postharvest fruit diseases

Ruíz-Moyano, Santiago; Martı́n, Alberto; Villalobos, M.C.; Calle, Alejandro; Serradilla, Manuel Joaquín; Córdoba, Maria de Guı́a; Hernández, Alejandro

doi:10.1016/j.fm.2016.01.003

Cited by 77 publications

(45 citation statements)

References 36 publications

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“…They formed the sixth, the seventh, the eight and the ninth group, respectively, each having a proportion of 8. [24,42,43].…”

Section: Identification Of Yeastsmentioning

confidence: 99%

“…At the same time, it is known that plant-associated microorganisms are extremely rich and have various biological functions: some of them can produce beneficial biologically active compounds [14,15], reduce the availability of heavy metals [16], improve the resistance of host plants to abiotic stresses [17,18], promote the agricultural disease resistance and have a growth-promoting action on plants [19][20][21][22]. It is believed that many of the plant-associated yeasts, like other microbes, have a definite biological function [23,24].…”

Section: Introductionmentioning

confidence: 99%

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Yeasts from Nanfeng mandarin plants: occurrence, diversity and capability to produce indole-3-acetic acid

Peng

Tang

et al. 2018

Biotechnology & Biotechnological Equipment

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Plant growth promoters produced by microorganisms can play a significant role in the induction of some important physiological responses in the growth and development of plants. In this study, we provided a first insight into revealing the diversity of cultivable yeasts associated with Nanfeng mandarin (Citrus reticulata cv. Blanco) in China. Their capability to produce indole-3acetic acid (IAA) was analyzed. A total of 796 yeast strains were obtained by the enrichment isolation technique from citrus soil, citrus leaves, citrus peel and citrus pulp of Nanfeng mandarin samples. On the basis of the 26S rDNA partial sequence analysis, the strains were identified as 14 yeast species in 9 genera belonging to Hanseniaspora sp., Pichia sp., Candida sp., Sporidiobolus sp., Meyerozyma sp., Symmetrospora sp., Rhodotorula sp., Starmerella sp. and Aureobasidium sp. The most abundant species in citrus soil were Starmerella meliponinorum and Meyerozyma caribbica. The species prevailing in citrus peel was Hanseniaspora opuntiae. Citrus pulp was rich in Meyerozyma guilliermondii. Additionally, Aureobasidium pullulans and H. opuntiae were the dominant species in citrus leaves. All yeast species obtained were accessed for the capability to produce IAA: 26 strains in seven species showed the capability of producing IAA. Rhodotorula paludigena produced the highest IAA concentrations of 76.22 mg/L. Our study confirms the phylogenetic diversity of yeast associated with Nanfeng mandarin and highlights that these yeast strains are promising resources of microbial fertilizer.

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“…They formed the sixth, the seventh, the eight and the ninth group, respectively, each having a proportion of 8. [24,42,43].…”

Section: Identification Of Yeastsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Yeasts from Nanfeng mandarin plants: occurrence, diversity and capability to produce indole-3-acetic acid

Peng

Tang

et al. 2018

Biotechnology & Biotechnological Equipment

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“…The main characteristics of an ideal biocontrol agent (BCA) are related to its biosafety, activity in a range of environments and against a variety of pathogens, and ease of management and use [65]. Extensive research has been developed to understand the mechanisms by which BCAs exert their action against pathogens.…”

Section: Antifungal Coatings For Fruit Preservationmentioning

confidence: 99%

Starch-Based Coatings for Preservation of Fruits and Vegetables

2018

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Considerable research has focused on the control of the physiological activity of fruits and vegetables in postharvest conditions as well as microbial decay. The use of edible coatings (ECs) carrying active compounds (e.g., antimicrobials) represents an alternative preservation technology since they can modify the internal gas composition by creating a modified atmosphere through the regulation of the gas exchange (oxygen, carbon dioxide, volatiles) while also limiting water transfer. Of the edible polymers able to form coating films, starch exhibits several advantages, such as its ready availability, low cost and good filmogenic capacity, forming colourless and tasteless films with high oxygen barrier capacity. Nevertheless, starch films are highly water sensitive and exhibit limited water vapour barrier properties and mechanical resistance. Different compounds, such as plasticizers, surfactants, lipids or other polymers, have been incorporated to improve the functional properties of starch-based films/coatings. This paper reviews the starch-based ECs used to preserve the main properties of fruits and vegetables in postharvest conditions as well as the different factors affecting the coating efficiency, such as surface properties or incorporation of antifungal compounds. The great variability in the plant products requires specific studies to optimize the formulation of coating forming products.

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“…Competition assays of 40 yeasts against a diverse set of 16 filamentous fungi identified M. pulcherrima as the overall strongest antagonist (4) and the same isolate was highly competitive against other antifungal yeasts on apples (5). The species exhibits strong antagonistic activity against apple postharvest diseases caused by Alternaria, Aspergillus, Botrytis, Fusarium, Monilinia, and Penicillium species (6)(7)(8)(9)(10)(11) and was also identified as a potential biocontrol organism against food-borne pathogens of freshly cut apples (12) as well as fungal grape diseases (13,14). Besides fungal pathogens of fruits, M. pulcherrima also inhibits other yeasts (15,16).…”

Section: Introductionmentioning

confidence: 99%

Snf2 controls pulcherriminic acid biosynthesis and connects pigmentation and antifungal activity of the yeastMetschnikowia pulcherrima

Gore‐Lloyd

Sumann

Brachmann

et al. 2018

Preprint

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Metschnikowia pulcherrima synthesizes the red pigment pulcherrimin, from cyclodileucine (cyclo(Leu-Leu)) as a precursor, and exhibits strong antifungal activity against notorious plant pathogenic fungi such as Botrytis and Gibberella (i.e., Fusarium). This yeast therefore has great potential for biocontrol applications against fungal diseases; particularly in the phyllosphere where this species is frequently found. To elucidate the molecular basis of the antifungal activity of M. pulcherrima, we compared a wildtype strain with a spontaneously occurring, pigmentless, weakly antagonistic mutant derivative. Whole genome sequencing of the wildtype and mutant strains identified a point mutation that creates a premature stop codon in the transcriptional regulator SNF2 in the mutant strain. Complementation of the snf2 mutant strain with the wildtype SNF2 gene restored pigmentation and recovered the strong antifungal activity of M. pulcherrima against plant pathogens in vitro and on cherries. Ultra-performance liquid chromatography-high resolution heated electrospray ionization mass spectrometry (UPLC HR HESI-MS) proved the presence and structure of the pulcherrimin precursors cyclo(Leu-Leu) and pulcherriminic acid and also identified new compounds that likely represented an additional precursor and degradation products of pulcherriminic acid and/or pulcherrimin. All of these compounds were identified in the wildtype and complemented strain, but were undetectable in the pigmentless snf2 mutant strain. These results thus identify SNF2 as a regulator of antifungal activity and pulcherriminic acid biosynthesis in M. pulcherrima and provide a starting point for deciphering the molecular functions underlying the antagonistic activity of this yeast. Significance statementMetschnikowia pulcherrima is a strongly antifungal yeast and a most promising species for the control of notorious plant diseases. This multidisciplinary study on the M. pulcherrima mode of action compared a wildtype isolate with a pigmentless mutant exhibiting reduced antifungal activity. The transcriptional regulator Snf2 was identified as a "biocontrol regulator" controlling antifungal activity of M. pulcherrima via PUL gene transcription, cyclodipeptide synthesis and additional, yet uncharacterized mechanisms. The identification of cyclo(Leu-Leu), pulcherriminic acid, as well as novel precursor and degradation products of pulcherrimin, opens up new avenues for research on the metabolism and functions of pulcherrimin. Overall, this works establishes M. pulcherrima as a genetically tractable model and will benefit the development of biocontrol solutions for important plant diseases. BIOLOGICAL SCIENCES, MicrobiologyGenome sequencing & mapping of mutations Genomic DNA of the M. pulcherrima strain APC 1.2 was extracted using the Qiagen DNeasy Plant Mini Kit and sequenced on the PacBio RS II platform (performed at the Functional Genomics Center Zurich). Subsequent de novo genome assembly, polishing and resequencing were performed using PacBio SMRT Portal 2.3.0...

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Yeasts isolated from figs (Ficus carica L.) as biocontrol agents of postharvest fruit diseases

Cited by 77 publications

References 36 publications

Yeasts from Nanfeng mandarin plants: occurrence, diversity and capability to produce indole-3-acetic acid

Yeasts from Nanfeng mandarin plants: occurrence, diversity and capability to produce indole-3-acetic acid

Starch-Based Coatings for Preservation of Fruits and Vegetables

Snf2 controls pulcherriminic acid biosynthesis and connects pigmentation and antifungal activity of the yeastMetschnikowia pulcherrima

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