Yeasts from Nanfeng mandarin plants: occurrence, diversity and capability to produce indole-3-acetic acid

Abstract

Plant growth promoters produced by microorganisms can play a significant role in the induction of some important physiological responses in the growth and development of plants. In this study, we provided a first insight into revealing the diversity of cultivable yeasts associated with Nanfeng mandarin (Citrus reticulata cv. Blanco) in China. Their capability to produce indole-3acetic acid (IAA) was analyzed. A total of 796 yeast strains were obtained by the enrichment isolation technique from citrus soil, citrus leaves, citrus peel and citrus pulp of Nanfeng mandarin samples. On the basis of the 26S rDNA partial sequence analysis, the strains were identified as 14 yeast species in 9 genera belonging to Hanseniaspora sp., Pichia sp., Candida sp., Sporidiobolus sp., Meyerozyma sp., Symmetrospora sp., Rhodotorula sp., Starmerella sp. and Aureobasidium sp. The most abundant species in citrus soil were Starmerella meliponinorum and Meyerozyma caribbica. The species prevailing in citrus peel was Hanseniaspora opuntiae. Citrus pulp was rich in Meyerozyma guilliermondii. Additionally, Aureobasidium pullulans and H. opuntiae were the dominant species in citrus leaves. All yeast species obtained were accessed for the capability to produce IAA: 26 strains in seven species showed the capability of producing IAA. Rhodotorula paludigena produced the highest IAA concentrations of 76.22 mg/L. Our study confirms the phylogenetic diversity of yeast associated with Nanfeng mandarin and highlights that these yeast strains are promising resources of microbial fertilizer.

A biostimulant yeast, Hanseniaspora opuntiae, modifies Arabidopsis thaliana root architecture and improves the plant defense response against Botrytis cinerea

“…In line with this, auxin reporter lines and pharmacological inhibition of auxin synthesis confirmed auxin-like effects. Moreover, these findings are supported by a previous report where H. opuntiae cells yeast exhibited the ability to produce indole-3-acetic acid (IAA) (Peng et al 2018 ); however, H. opuntiae indirectly may affect plant growth by competing for both space and nutrients, since during direct interaction Arabidopsis fresh weight is reduced.…”

Use of Non-Saccharomyces Yeast Co-Fermentation with Saccharomyces cerevisiae to Improve the Polyphenol and Volatile Aroma Compound Contents in Nanfeng Tangerine Wines

“…Nanfeng tangerine (Citrus reticulata Blanco) is a well-known delicacy used in China for its flavor and nutrition with a long cultivation history (about 1300 years) and widespread production [1,2]. Tangerine wines not only retain the flavor of Nanfeng tangerines but are also associated with the production of a variety of flavor components and biologically active compounds during the alcoholic fermentation process [3], including polyphenols [4].…”

Molecular characterization of endophytic fungal communities associated with Vitis vinifera L. at Taif region of Saudi Arabia

“…For examples, three species of Hanseniaspora (H. uvarum, H. thailandica and H. opuntiae) were detected as endophyte in leaves of Nanfeng mandarin (Citrus reticulata cv. Blanco) in China (Peng et al, 2018). Also, H. uvarum was detected in the leaf galls formed by Pontania sawflies in four different plant species, especially at final stage of gall formation during July and September with an average relative abundance ranging from 1.21 to 9.45% (Glushakova and Kachalkin, 2017).…”