Yeast Surface Display of Capsid Protein VP7 of Grass Carp Reovirus: Fundamental Investigation for the Development of Vaccine Against Hemorrhagic Disease

Luo, Shaoxiang; Yan, Liming; Zhang, Xiaohua; Li, Yuan; Qin, Fang; Zhang, Yongan; Dai, Heping

doi:10.4014/jmb.1505.05041

Cited by 21 publications

(9 citation statements)

References 44 publications

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“…The yeast cell surface is a functional interface where natively displayed molecules on the surface play important roles in various biological or physiological processes [ 68 ]. Thus, the strategy of displaying target proteins on the yeast surface is currently used as an efficient vaccine delivery system [ 69 , 70 ]. Therefore, the elicited immune response due to oral administration of LTB-scEDIII WC could be improved through the surface display of scEDIII, thus enhancing mucosal targeting of the target antigen.…”

Section: Discussionmentioning

confidence: 99%

Comparative immunogenicity of preparations of yeast-derived dengue oral vaccine candidate

et al. 2018

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BackgroundDengue is listed as a neglected tropical disease by the Center for Disease Control and Preservation, as there are insufficient integrated surveillance strategies, no effective treatment, and limited licensed vaccines. Consisting of four genetically distinct serotypes, dengue virus (DENV) causes serious life-threatening infections due to its complexity. Antibody-dependent enhancement by pre-existing cross-reactive as well as homotypic antibodies further worsens the clinical symptoms of dengue. Thus, a vaccine conferring simultaneous and durable immunity to each of the four DENV serotypes is essential to restrict its escalation. In deeply affected resource-limited countries, oral vaccination using food-grade organisms is considered to be a beneficial approach in terms of costs, patient comfort, and simple logistics for mass immunization. The current study used a mouse model to explore the immunogenicity of an oral dengue vaccine candidate prepared using whole recombinant yeast cells (WC) and cell-free extracts (CFE) from cells expressing recombinant Escherichia coli heat-labile toxin protein B-subunit (LTB) fused to the consensus dengue envelope domain III (scEDIII). Mice were treated orally with recombinant WC and CFE vaccines in 2-week intervals for 4 weeks and changes in systemic and mucosal immune responses were monitored.ResultsBoth WC and CFE dosage applications of LTB-scEDIII stimulated a systemic humoral immune response in the form of dengue-specific serum IgG as well as mucosal immune response in the form of secretory sIgA. Antigen-specific B cell responses in isolated lymphoid cells from the spleen and Peyer’s patches further indicated an elevated mucosal immune response. Cellular immune response estimated through lymphocyte proliferation assay indicated higher levels in CFE than WC dosage. Furthermore, sera obtained after both oral administrations successfully neutralized DENV-1, whereas CFE formulation only neutralized DENV-2 serotype, two representative serotypes which cause severe dengue infection. Sera from mice that were fed CFE preparations demonstrated markedly higher neutralizing titers compared to those from WC-fed mice. However, WC feeding elicited strong immune responses, which were similar to the levels induced by CFE feeding after intraperitoneal booster with purified scEDIII antigen.ConclusionsCFE preparations of LTB-scEDIII produced strong immunogenicity with low processing requirements, signifying that this fusion protein shows promise as a potent oral vaccine candidate against dengue viral infection.Electronic supplementary materialThe online version of this article (10.1186/s12934-018-0876-0) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Comparative immunogenicity of preparations of yeast-derived dengue oral vaccine candidate

et al. 2018

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“…VP7, an outer capsid protein of grass carp reovirus (GCRV), was expressed and displayed on the surface of Saccharomyces cerevisiae for developing an efficient vaccine against haemorrhagic disease of grass carp (Luo et al . 2015). Genetically engineered Pichia pastoris could display biologically active viral binding protein Rab7 and binding partner envelope protein VP28 from WSSV, and was ready for evaluation of efficacy in protecting shrimp against WSSV by oral administration (Ananphongmanee et al .…”

Section: Application In Aquaculturementioning

confidence: 99%

Surface display system for probiotics and its application in aquaculture

Yao

Yang

Gao

et al. 2020

Reviews in Aquaculture

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Aquaculture is a fast‐growing and rapidly expanding industry in the world. Probiotics are widely used in aquaculture and have provided benefits to aquatic animal health, and it is also a promising alternative to antibiotics for control of fish diseases. With the development of biotechnology, new expression systems and novel techniques for the surface display of heterologous proteins in surface of probiotics cells have been developed. This review provides an overview of the systems and strategies for displaying functional proteins on the surface of probiotics commonly used in aquaculture, which are Bacillus subtilis, Bacillus thuringiensis, lactic acid bacteria represented by Lactococcus lactis and yeast. Their applications in aquaculture especially for oral vaccine development afforded by this technology and prospects and challenges associated with this technology are also highlighted.

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“…Recent advances in human health care include a vaccine against candidiasis ( Shibasaki et al, 2013 ; Ueda, 2016 ) and a platform to detect SARS-CoV-2 ( Maneira et al, 2021 ). Animal vaccines have been developed which include vaccines against Toxoplasma gondii ( Wang et al, 2018 ), a vaccine against hemorrhagic disease of grass carp ( Luo et al, 2015 ) and an anti-tick vaccine ( Trentelman et al, 2021 ). Other relevant applications are the development of antibiotics ( Chun et al, 2020 ), biosensors to detect blood biochemical parameters ( Zhao et al, 2020b ) and the expression of hydrophobins ( Andreu et al, 2021 ).…”

Section: Strategies To Improve Yeast Surface Displaymentioning

confidence: 99%

Yeast Surface Display System: Strategies for Improvement and Biotechnological Applications

Teymennet-Ramírez

Martínez‐Morales

Trejo‐Hernández

2022

Front. Bioeng. Biotechnol.

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Yeast surface display (YSD) is a “whole-cell” platform used for the heterologous expression of proteins immobilized on the yeast’s cell surface. YSD combines the advantages eukaryotic systems offer such as post-translational modifications, correct folding and glycosylation of proteins, with ease of cell culturing and genetic manipulation, and allows of protein immobilization and recovery. Additionally, proteins displayed on the surface of yeast cells may show enhanced stability against changes in temperature, pH, organic solvents, and proteases. This platform has been used to study protein-protein interactions, antibody design and protein engineering. Other applications for YSD include library screening, whole-proteome studies, bioremediation, vaccine and antibiotics development, production of biosensors, ethanol production and biocatalysis. YSD is a promising technology that is not yet optimized for biotechnological applications. This mini review is focused on recent strategies to improve the efficiency and selection of displayed proteins. YSD is presented as a cutting-edge technology for the vectorial expression of proteins and peptides. Finally, recent biotechnological applications are summarized. The different approaches described herein could allow for a better strategy cascade for increasing protein/peptide interaction and production.

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Yeast Surface Display of Capsid Protein VP7 of Grass Carp Reovirus: Fundamental Investigation for the Development of Vaccine Against Hemorrhagic Disease

Cited by 21 publications

References 44 publications

Comparative immunogenicity of preparations of yeast-derived dengue oral vaccine candidate

Comparative immunogenicity of preparations of yeast-derived dengue oral vaccine candidate

Surface display system for probiotics and its application in aquaculture

Yeast Surface Display System: Strategies for Improvement and Biotechnological Applications

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