Surface display system for probiotics and its application in aquaculture

Abstract

Aquaculture is a fast‐growing and rapidly expanding industry in the world. Probiotics are widely used in aquaculture and have provided benefits to aquatic animal health, and it is also a promising alternative to antibiotics for control of fish diseases. With the development of biotechnology, new expression systems and novel techniques for the surface display of heterologous proteins in surface of probiotics cells have been developed. This review provides an overview of the systems and strategies for displaying functional proteins on the surface of probiotics commonly used in aquaculture, which are Bacillus subtilis, Bacillus thuringiensis, lactic acid bacteria represented by Lactococcus lactis and yeast. Their applications in aquaculture especially for oral vaccine development afforded by this technology and prospects and challenges associated with this technology are also highlighted.

Analysis of the Productivity, Immunity, and Health Performance of Nile Tilapia (Oreochromis niloticus) Broodstock-fed Dietary Fermented Extracts Sourced from Saccharomyces cerevisiae (Hilyses): A Field Trial

“…This finding may be due to the expression of anti-inflammatory cytokines and increased antioxidant enzyme activity [ 32 ]. Several articles have indicated that upregulation of proinflammatory cytokines improves immune promptness and increases disease resistance in tilapia [ 9 , 18 , 61 ].…”

“…Other additives, such as probiotics, prebiotics, phytogenics, dietary acidifiers and immunostimulants, directly affect animal performance and health [ 6 , 7 , 8 ]. Functional feeds containing gut health promotors have become a key component of any strategy to prevent diseases in aquaculture, particularly when opportunistic bacteria are suspected to be a major cause of mortality [ 9 , 10 ]. Gut health promotors combine different modes of action, including modulating the intestinal microbial flora, stimulating the local immunity of the gut and improving the histological intestinal structure [ 11 ].…”

“…Broodstock nutrition is recognized as a major factor that can influence fish reproduction and subsequent larval quality [ 15 ]. Many studies have indicated the positive effects of dietary fortification of whole yeast and/or its by-products on the growth performance, hematological indices, antioxidant defense systems, immune response, and disease resistance in Nile tilapia [ 9 , 16 , 17 , 18 , 19 ]. Abu-Elala et al [ 5 ] recorded that dietary fermented yeast extracts sourced from Saccharomyces cerevisiae successfully enhanced the phagocytic activity/index, and lysozyme activity up-regulated the proinflammatory cytokines ( TNF-α and IL-1β ) and increased the resistance of fish against bacterial diseases.…”

A mini-review: Effect of probiotics on the growth and health of fish

“…Probiotics can be used as an alternative for controlling diseases in fish caused by pathogenic microorganisms [43] . As according to Sadeghi et al (2021) [44] and Yao et al (2020) [45] probiotics have a beneficial effect on gut health and innate immunity, due to the live nature of probiotics that can compete with pathogenic microorganisms and colonize in the digestive tract. Multispecies can help increase specific or non-specific immune systems in fish, by inducing phagocytic cells, lysozymes and various cytokinins [46] .…”

Plant-Produced Vaccines: Future Applications in Aquaculture

“…Therefore, health management of aquaculture is of great importance for food security, food safety, the environment, and sustainable development of the aquaculture industry. Moreover, candidate alternatives, e.g., probiotics and plant bioactive compounds to replace antibiotics, could also be promising, contributing to the management of aquaculture fish health (Yao et al, 2020).…”