Yeast mitophagy: Unanswered questions

Huang, Yuxiang; Klionsky, Daniel J.

doi:10.1016/j.bbagen.2021.129932

Cited by 7 publications

(7 citation statements)

References 118 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, it is possible that thiol-containing proteins targeted by glutathione could play a role in the regulation of mitophagy. There is also an unanswered question as to whether glutathione could play a role in clearing mitochondrially generated oxidants in vacuoles in the final step of mitophagy [ 72 ].…”

Section: Are Ros the Main Culprits?mentioning

confidence: 99%

Mitophagy in Yeast: Decades of Research

Bhatia‐Kiššová

Camougrand

2021

Cells

View full text Add to dashboard Cite

Mitophagy, the selective degradation of mitochondria by autophagy, is one of the most important mechanisms of mitochondrial quality control, and its proper functioning is essential for cellular homeostasis. In this review, we describe the most important milestones achieved during almost 2 decades of research on yeasts, which shed light on the molecular mechanisms, regulation, and role of the Atg32 receptor in this process. We analyze the role of ROS in mitophagy and discuss the physiological roles of mitophagy in unicellular organisms, such as yeast; these roles are very different from those in mammals. Additionally, we discuss some of the different tools available for studying mitophagy.

show abstract

Section: Are Ros the Main Culprits?mentioning

confidence: 99%

Mitophagy in Yeast: Decades of Research

Bhatia‐Kiššová

Camougrand

2021

Cells

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

“…There are two pathways, the ubiquitin-dependent pathway and receptor-dependent pathway, for mitophagy to serve as a critical quality-control mechanism for selective targeting and removal of damaged or dysfunctional mitochondria ( Huang and Klionsky, 2021 ; Nakamura, et al, 2021 ). The two pathways have been identified in yeast, plants, and mammals ( Gan, et al, 2018 ; Gkikas, et al, 2018 ; Huang and Klionsky, 2021 ; Nakamura, et al, 2021 ; Ren, et al, 2021 ). The ubiquitin-dependent mitophagy is characterized by PINK1/Parkin-mediated ubiquitination of the mitochondrial proteins, including optineurin (OPTN), sequestosome 1 (SQSTM1, also known as P62), TAX1-binding protein 1 (TAX1BP1), nuclear domain 10 protein 52 (NDP52), voltage-dependent anion channel (VDAC), and mitofusin (MFN) ( Huang and Klionsky, 2021 ; Nakamura, et al, 2021 ; Ren, et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…The two pathways have been identified in yeast, plants, and mammals ( Gan, et al, 2018 ; Gkikas, et al, 2018 ; Huang and Klionsky, 2021 ; Nakamura, et al, 2021 ; Ren, et al, 2021 ). The ubiquitin-dependent mitophagy is characterized by PINK1/Parkin-mediated ubiquitination of the mitochondrial proteins, including optineurin (OPTN), sequestosome 1 (SQSTM1, also known as P62), TAX1-binding protein 1 (TAX1BP1), nuclear domain 10 protein 52 (NDP52), voltage-dependent anion channel (VDAC), and mitofusin (MFN) ( Huang and Klionsky, 2021 ; Nakamura, et al, 2021 ; Ren, et al, 2021 ). The receptor-dependent mitophagy depends on diverse mitophagy receptors, including BCL2 interacting protein 3 (BNIP3), Nip3-like protein X (NIX), FUN14 domain-containing protein 1 (FUNDC1), cardiolipin, and prohibitin 2 (PHB2) ( Huang and Klionsky, 2021 ; Nakamura, et al, 2021 ; Ren, et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

LC3-Mediated Mitophagy After CCCP or Vibrio splendidus Exposure in the Pacific Oyster Crassostrea gigas

Sun

Leng

et al. 2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Mitochondrial selective autophagy, known as mitophagy, surveils the mitochondrial population by eliminating superfluous and/or impaired organelles to mediate cellular survival and viability in response to injury/trauma and infection. In this study, the components of the mitophagy pathway in the Pacific oyster Crassostrea gigas were screened from NCBI with reference to the protein sequences of the human mitophagy process. A total of 10 mitophagy process–related genes were identified from C. gigas, including NIX, FUNDC1, PHB2, Cardiolipin, P62, VDAC2, MFN2, PARL, MPP, and OPTN. They shared high similarities with their homologs in the human mitophagy pathway and were expressed in various tissues of C. gigas. After CCCP exposure, the fluorescence intensity of the mitochondrial probe JC-1 monomers increased significantly in hemocytes, while the fluorescence intensity of JC-1 aggregates decreased significantly. Meanwhile, the fluorescence of lysosomes was found to be co-localized with that of CgLC3 and mitochondria in CCCP-treated hemocytes. Double- and single-membrane-bound vacuoles resembling autophagic structures were observed in the hemocytes after CCCP exposure. The fluorescence intensity of JC-1 monomers and the abundance of CgLC3Ⅱ in hemocytes both increased after Vibrio splendidus exposure. At the same time, the green signals of CgLC3 were co-localized with red signals of the mitochondria, and the fluorescence intensity of autophagy increased significantly in hemocytes after V. splendidus exposure. The results confirmed the existence of a complete mitophagy pathway in mollusks for the first time, which was helpful for further study on the function of mitochondrial autophagy in mollusks.

show abstract

“…In yeast, Atg32, an outer mitochondrial membrane (OMM) protein, serves as the receptor recruiting Atg8, and Atg11 acts as the scaffold/platform for core Atg protein assembly [ 13 , 14 , 15 , 16 ]. Mammalian mitophagy is more complicated and occurs via the action of different components depending on the cell type and/or purpose; for example, mitochondrial removal during red blood cell erythropoiesis involves BNIP3L/NIX and is part of a normal developmental pathway [ 17 ].…”

Section: Introduction To Autophagymentioning

confidence: 99%

The Emerging Roles of Autophagy in Human Diseases

Lei

Klionsky

2021

Biomedicines

Self Cite

View full text Add to dashboard Cite

Autophagy, a process of cellular self-digestion, delivers intracellular components including superfluous and dysfunctional proteins and organelles to the lysosome for degradation and recycling and is important to maintain cellular homeostasis. In recent decades, autophagy has been found to help fight against a variety of human diseases, but, at the same time, autophagy can also promote the procession of certain pathologies, which makes the connection between autophagy and diseases complex but interesting. In this review, we summarize the advances in understanding the roles of autophagy in human diseases and the therapeutic methods targeting autophagy and discuss some of the remaining questions in this field, focusing on cancer, neurodegenerative diseases, infectious diseases and metabolic disorders.

show abstract

Yeast mitophagy: Unanswered questions

Cited by 7 publications

References 118 publications

Mitophagy in Yeast: Decades of Research

Mitophagy in Yeast: Decades of Research

LC3-Mediated Mitophagy After CCCP or Vibrio splendidus Exposure in the Pacific Oyster Crassostrea gigas

The Emerging Roles of Autophagy in Human Diseases

Contact Info

Product

Resources

About