Yeast cell factories for fine chemical and API production

Pscheidt, Beate; Glieder, Anton

doi:10.1186/1475-2859-7-25

Cited by 104 publications

(69 citation statements)

References 249 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, a range of commercial manufacturing processes relies on the formation of microbial biofilms (23,25,26). Hence, it may be possible to adapt antibody-mediated immobilization to promote biofilm formation to exploit the industrial use of these microbes.…”

Section: Vol 75 2009 Fungal Cell Immobilization Promotes Biofilm Fomentioning

confidence: 99%

Antibody-Mediated Immobilization of Cryptococcus neoformans Promotes Biofilm Formation

Robertson

Casadevall

2009

Appl Environ Microbiol

View full text Add to dashboard Cite

Most microbes, including the fungal pathogen Cryptococcus neoformans, can grow as biofilms. Biofilms confer upon microbes a range of characteristics, including an ability to colonize materials such as shunts and catheters and increased resistance to antibiotics. Here, we provide evidence that coating surfaces with a monoclonal antibody to glucuronoxylomannan, the major component of the fungal capsular polysaccharide, immobilizes cryptococcal cells to a surface support and, subsequently, promotes biofilm formation. We used time-lapse microscopy to visualize the growth of cryptococcal biofilms, generating the first movies of fungal biofilm growth. We show that when fungal cells are immobilized using surface-attached specific antibody to the capsule, the initial stages of biofilm formation are significantly faster than those on surfaces with no antibody coating or surfaces coated with unspecific monoclonal antibody. Time-lapse microscopy revealed that biofilm growth was a dynamic process in which cells shuffled position during budding and was accompanied by emergence of planktonic variant cells that left the attached biofilm community. The planktonic variant cells exhibited mobility, presumably by Brownian motion. Our results indicate that microbial immobilization by antibody capture hastens biofilm formation and suggest that antibody coating of medical devices with immunoglobulins must exclude binding to common pathogenic microbes and the possibility that this effect could be exploited in industrial microbiology.

show abstract

Section: Vol 75 2009 Fungal Cell Immobilization Promotes Biofilm Fomentioning

confidence: 99%

Antibody-Mediated Immobilization of Cryptococcus neoformans Promotes Biofilm Formation

Robertson

Casadevall

2009

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Whole-cell biotransformations with recombinant microbes offer many advantages with respect to scalable metabolite production, and corresponding expression systems for human P450s have thus been established in bacteria and yeasts (Ghisalba and Kittelmann, 2007;Pscheidt and Glieder, 2008). In recent years, we showed the usefulness of recombinant strains of the fission yeast Schizosaccharomyces pombe that express human P450s for the production of P450 metabolites of illicit drugs (Peters et al, 2009) and doping substances (Zöllner et al, in press).…”

mentioning

confidence: 99%

Glucuronide Production by Whole-Cell Biotransformation Using Genetically Engineered Fission YeastSchizosaccharomyces pombe

Drăgan¹,

Buchheit²,

Bischoff³

et al. 2009

Drug Metab Dispos

View full text Add to dashboard Cite

ABSTRACT:Drug metabolites generated by UDP glycosyltransferases (UGTs) are needed for drug development and toxicity studies, especially in the context of safety testing of metabolites during drug development. Because chemical metabolite synthesis can be arduous, various biological approaches have been developed; however, no whole-cell biotransformation with recombinant microbes that express human UGTs was yet achieved. In this study we expressed human UDP glucose-6-dehydrogenase together with several human or rat UGT isoforms in the fission yeast Schizosaccharomyces pombe and generated strains that catalyze the whole-cell glucuronidation of standard substrates. Moreover, we established two methods to obtain stable isotope-labeled glucuronide metabolites: the first uses a labeled aglycon, whereas the second uses 13 C 6 -glucose as a metabolic precursor of isotope-labeled UDP-glucuronic acid and yields a 6-fold labeled glucuronide. The system described here should lead to a significant facilitation in the production of both labeled and unlabeled drug glucuronides for industry and academia.

show abstract

“…La genómica funcional también ha dado lugar a una nueva visión de la ingeniería genética dándole un potencial más amplio convirtiéndose, más bien, en una "ingeniería genómica" (Carr & Church 2009), pasando de la ingeniería metabólica (Graham et al 2007, Lee et al 2009, Stephanopoulos 2002, Ye & Bathia 2012 y la evolución dirigida (Chatterjee & Yuan 2006, Lin et al 2009, Turner 2003) a la construcción de "factorías celulares" (Barnes & Dickson 2006, Mapari et al 2009, Pscheidt & Glieder 2008, Villaverde 2010) y recientemente a la biología sintética (Aldrich et al 2008, Endy 2008, Gibson et al 2010, Sismour & Benner 2005. Otro desarrollo que vale la pena resaltar es el advenimiento de la metagenómica que está permitiendo por un lado, estudiar y entender más profundamente la ecología de microorganismos y, de otro, la identificación de nuevas especies microbianas hasta ahora elusivas a su estudio (Langer et al 2006, Riesenfeld et al 2004, Schmeisser et al 2007, Warnecke & Hugenholtz 2007.…”

Section: Algunos Logros En Biotecnologíaunclassified

El camino de la biotecnología en la Universidad Nacional Agraria La Molina, Perú

Gutiérrez-Correa

2013

Rev peru biol

View full text Add to dashboard Cite

COMENTARIO Presentado:26/11/2012 Aceptado:15/12/2012 Publicado online: 15/01/2013 ResumenSe realiza un relato histórico sobre los cincuenta años que tiene la carrera de biología en la Universidad Nacional Agraria La Molina y la gestación, inicio, desarrollo y maduración de la biotecnología en esta universidad. Se describen algunos pormenores de las tres grandes estructuraciones curriculares que finalmente derivan al establecimiento del área de biotecnología en el pregrado y en el doctorado así como a los logros alcanzados. El desarrollo de la biotecnología en la UNALM ha influenciado el desarrollo de esta área en el Perú no solo en las adaptaciones curriculares que se han producido en varias universidades del país sino también en las investigaciones biotecnológicas que se llevan a cabo en universidades e institutos de investigación en las diferentes regiones del país. En este devenir histórico se concluye que el balance es muy positivo y que la mejor corriente epistemológica es la que nos lleva mediante la investigación a encontrar verdades usables en la solución de los problemas del país y, también por qué no, del planeta.Palabras clave: historia de la biología en el Perú; biotecnología; universidad; curriculum. AbstractIn this paper, I make a historical account of the fifty years that have the degree in biology at the Universidad Nacional Agraria La Molina and gestation, and the beginning, development and maturation of biotechnology in this university. We describe some details of the three most important curricular modifications that led to the establishment of the biotechnology area in the curricula of undergraduate and doctoral programs, as well as their main achievements. The development of biotechnology at the UNALM has influenced the development of this area in Peru not only in the curricular changes that have occurred in several Peruvian universities, but also in the biotechnology research being carried out in many regions of the country. In this historical process, I conclude that the balance is positive and that the best epistemology stream is that which leads through research to find truths usable for solving the problems of Peru and also why not the planet.Keywords: history of biology in Peru; biotechnology; university; curriculum. Amicus Plato sed magis amica veritas -AristótelesCaminante, no hay camino, se hace camino al andar -Antonio Machado IntroducciónHan transcurrido cincuenta años desde que en 1962 se creó la Carrera de Biología en la Facultad de Ciencias de la Universidad Nacional Agraria La Molina (UNALM). Mi relación con la carrera lleva 44 años, primero como estudiante desde 1968 y luego como profesor desde 1975 (37 años) por lo que me ha tocado ser parte de su desarrollo histórico, incluyendo el periodo 1988 a 1992 como presidente de la Comisión de Currículo de la Facultad de Ciencias encargada de la última gran reestructuración curricular. Es indudable que relatar la historia es una tarea extremadamente difícil por el inevitable sesgo del relator, aunque el respetar los hechos y las evi...

show abstract

Yeast cell factories for fine chemical and API production

Cited by 104 publications

References 249 publications

Antibody-Mediated Immobilization of Cryptococcus neoformans Promotes Biofilm Formation

Antibody-Mediated Immobilization of Cryptococcus neoformans Promotes Biofilm Formation

Glucuronide Production by Whole-Cell Biotransformation Using Genetically Engineered Fission YeastSchizosaccharomyces pombe

El camino de la biotecnología en la Universidad Nacional Agraria La Molina, Perú

Contact Info

Product

Resources

About