Yeast artificil chromosome (YAC) clones and sequence tagged site (STS) markers anchored at human chromosome 21

Eki, Toshihiko; Yokoyama, Kazushige; Tashiro, Hiroyuki; Ozawa, Kazuo; Murakami, Yasufumi; Watkins, Paul C.; Soeda, Eiichi

doi:10.1007/bf01883607

Cited by 4 publications

(1 citation statement)

References 18 publications

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“…We previously generated STSs specific to Chr 21 and isolated YACs anchored at these loci around the 21q22.1 region (Tang et al 1992;Eki et al 1991;Abe et al 1995). More recently, we reported a high-resolution physical map of the 1.8-Mb region at the distal portion of the 21q22.1 region, which encompasses the interferon-c~ receptor gene (IFNAR) and AML1 loci (Eki et al 1996).…”

Section: Introductionmentioning

confidence: 97%

A long-range physical map of human Chromosome 21q22.1 band from the YAC continuum

et al. 1996

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The human Chromosome (Chr) 21q22.1 region contains several genes for cytokines and neurotransmitters and the gene for superoxide dismutase (mutant forms of which can cause familial amyotrophic lateral sclerosis). A region of approximately 5.8 Mb encompassing D21S82 and the glycinamide ribonucleotide transformylase (GART) loci was covered by overlapping YAC clones, which were contiguously ordered by clone walking with sequence-tagged site (STSs). A total of 76 markers, including 29 YAC end-specific STSs, were unambiguously ordered in this 5.8-Mb region, and the average interval between markers was 76 kb. Restriction maps of the YAC clones with rare-cutting enzymes were simultaneously prepared, and the restriction sites were aligned to obtain a consensus restriction map of the proximal region of the 21q22.1 band. The restriction map made from 44 overlapping YACs contains 54 physically assigned STSs. By integrating the consensus map of the adjacent 1.8-Mb region, we obtained a fine physical map spanning 6.5 Mb of human Chr 21q22.1. This map contains 24 precisely positioned end-specific STSs and 12 NotI-linking markers. More than 39 potential CpG islands were identified in this region and were found to be unevenly distributed. This physical map and the YACs should be useful as a reference map and as a resource for further structural analysis of the Giemsa-negative band (R-band) of Chr 21q22.1.

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Section: Introductionmentioning

confidence: 97%

A long-range physical map of human Chromosome 21q22.1 band from the YAC continuum

et al. 1996

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Construction and characterization of a rice YAC library for physical mapping

et al. 1995

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Generation of 28 sequence-tagged sites (STSs) from yeast artificial chromosome (YAC) clones anchored at human chromosome 21q22.1 region

et al. 1995

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Twenty-eight sequence-tagged sites (STSs) were newly generated from the DNA sequences of vector-insert junctions from yeast artificial chromosomes (YACs) anchored at chromosome 21q22.1 region. The insert DNAs adjacent to vector arms were specifically amplified through inverse PCR method to clone into pUC19 vector for sequencing. Sixty DNA junctions from 44 CEPH YAC clones were cloned and sequenced. Of these DNA sequences of junctions between vector-arms and DNA inserts, twenty-eight STSs were finally obtained to show the accurate amplification, which is specific for human chromosome 21. The sets of 28 STSs were useful to build fine YAC contigs by STS-mediated YAC walking at the 21q22.1 region.

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Yeast artificil chromosome (YAC) clones and sequence tagged site (STS) markers anchored at human chromosome 21

Cited by 4 publications

References 18 publications

A long-range physical map of human Chromosome 21q22.1 band from the YAC continuum

A long-range physical map of human Chromosome 21q22.1 band from the YAC continuum

Construction and characterization of a rice YAC library for physical mapping

Generation of 28 sequence-tagged sites (STSs) from yeast artificial chromosome (YAC) clones anchored at human chromosome 21q22.1 region

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