Yeast AEP3p Is an Accessory Factor in Initiation of Mitochondrial Translation

Lee, Changkeun; Tibbetts, Anne S.; Kramer, Gisela; Appling, Dean R.

doi:10.1074/jbc.m109.055350

Cited by 22 publications

(34 citation statements)

References 64 publications

(74 reference statements)

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“…Simultaneous disruption of both FMT1 and AEP3 genes leads to a synthetic respiratory defect – a phenotype even more severe than that seen in fmt -deficient E. coli [23]. In vitro experiments have shown that complex formation between Aep3p and mIF2 promotes the binding of Met-tRNA i fMet – but not of fMet-tRNA i fMet – to mIF2, thus promoting Met-tRNA i fMet use in initiation.…”

Section: Mitochondrial Initiation Factor 2 (Mif2)mentioning

confidence: 99%

Mitochondrial translation initiation machinery: Conservation and diversification

et al. 2014

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The highly streamlined mitochondrial genome encodes almost exclusively a handful of transmembrane components of the respiratory chain complex. In order to ensure the correct assembly of the respiratory chain, the products of these genes must be produced in the correct stoichiometry and inserted into the membrane, posing a unique challenge to the mitochondrial translational system. In this review we describe the proteins orchestrating mitochondrial translation initiation: bacterial-like general initiation factors mIF2 and mIF3, as well as mitochondria-specific components – mRNA-specific translational activators and mRNA-nonspecific accessory initiation factors. We consider how the fast rate of evolution in these organelles has not only created a system that is divergent from that of its bacterial ancestors, but has led to a huge diversity in lineage specific mechanistic features of mitochondrial translation initiation among eukaryotes.

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Section: Mitochondrial Initiation Factor 2 (Mif2)mentioning

confidence: 99%

Mitochondrial translation initiation machinery: Conservation and diversification

et al. 2014

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“…The ability of the yeast mitochondrial translational system to function without formylation of Met-tRNA is dependent on the presence of a factor designated Aep3p that is thought to interact with yeast IF2 mt and facilitates its use of Met-tRNA [57]. There is no clear homolog of this protein in mammals although it has a weak homology to the small subunit ribosomal protein MRPS27 (e = 9.3×10 −2 ).…”

Section: Initiation Of Protein Synthesis In Mammalian Mitochondriamentioning

confidence: 99%

Mechanism of protein biosynthesis in mammalian mitochondria

Christian

Spremulli

2012

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanism

179

143

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Protein synthesis in mammalian mitochondria produces 13 proteins that are essential subunits of the oxidative phosphorylation complexes. This review provides a detailed outline of each phase of mitochondrial translation including initiation, elongation, termination, and ribosome recycling. The roles of essential proteins involved in each phase are described. All of the products of mitochondrial protein synthesis in mammals are inserted into the inner membrane. Several proteins that may help bind ribosomes to the membrane during translation are described, although much remains to be learned about this process. Mutations in mitochondrial or nuclear genes encoding components of the translation system often lead to severe deficiencies in oxidative phosphorylation, and a summary of these mutations is provided.

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“…Aep3 would allow the use of unformylated methionine as initiator amino acid by promoting the binding of unformylated Met-mt-tRNA to mt-IF2. 41 Whether this additional function is linked to the presence of the PPR motifs or rather to an additional domain of the protein has not yet been investigated.…”

Section: Principal Steps Of Mitochondrial Gene Expression Affected Bymentioning

confidence: 99%