Year, Location, and Variety Impact on Grape-Associated Mycobiota of Arkansas-Grown Wine Grapes for Wine Production

Cureau, Natacha; Threlfall, Renee T.; Marasini, Daya; Lavefve, Laura; Carbonero, Franck

doi:10.1007/s00248-021-01705-y

Cited by 11 publications

(5 citation statements)

References 54 publications

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“…In these four vineyards, microbial enzymatic activities were lower in symptomatic soils compared to asymptomatic soils, suggesting a microbial dysbiosis in areas subjected to decline. Year of sampling was pointed out as a major factor influencing the diversity and composition of the microbiota ( Berlanas et al, 2019 ; Cureau et al, 2021 ). We observed here that while microbial communities evolved from year to year, the differences in microbial composition between S and AS soils was confirmed over the 2 years of sampling, with more putative fungal pathogens in S soils.…”

Section: Discussionmentioning

confidence: 99%

Soil composition and rootstock genotype drive the root associated microbial communities in young grapevines

et al. 2022

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Soil microbiota plays a significant role in plant development and health and appears to be a major component of certain forms of grapevine decline. A greenhouse experiment was conducted to study the impact of the microbiological quality of the soil and grapevine rootstock genotype on the root microbial community and development of young plants. Two rootstocks heterografted with the same scion were grown in two vineyard soils differing in microbial composition and activities. After 4 months, culture-dependent approaches and amplicon sequencing of bacterial 16S rRNA gene and fungal ITS were performed on roots, rhizosphere and bulk soil samples. The root mycorrhizal colonization and number of cultivable microorganisms in the rhizosphere compartment of both genotypes were clearly influenced by the soil status. The fungal diversity and richness were dependent on the soil status and the rootstock, whereas bacterial richness was affected by the genotype only. Fungal genera associated with grapevine diseases were more abundant in declining soil and related root samples. The rootstock affected the compartmentalization of microbial communities, underscoring its influence on microorganism selection. Fluorescence in situ hybridization (FISH) confirmed the presence of predominant root-associated bacteria. These results emphasized the importance of rootstock genotype and soil composition in shaping the microbiome of young vines.

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Section: Discussionmentioning

confidence: 99%

Soil composition and rootstock genotype drive the root associated microbial communities in young grapevines

et al. 2022

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“…This means that the members of Cellvibrio might hurt plant cells. The yeast Filobasidium has been described as an important member of the wine consortia for its role in creating specific flavor profiles or reducing final alcohol content (Cureau et al, 2021). At the species level, the relative abundance of unclassified_norank_norank_Chloroplast and unclassified_Penicillium have shown significant differences between the two groups of samples.…”

Section: Discussionmentioning

confidence: 99%

Metabolome and Microbiome Signatures in the Leaves of Wild Tea Plant Resources Resistant to Pestalotiopsis theae

Zhang

Chang-yu

et al. 2022

Front. Microbiol.

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Tea (Camellia sinensis) is an important crop that is mainly used in the food industry. This study using the metabolome and microbiome investigates the resistance factors of wild tea plant resources against tea gray blight disease, which is caused by Pestalotiopsis theae (Sawada) Steyaert. According to the interaction analysis of tea leaves and pathogenic fungus, the resistance of wild tea plant resource “R1” (Resistance 1) to tea gray blight disease was significantly higher than that of wild tea plant resource “S1” (Susceptibility 1). The difference between “R1” and “S1” in the metabolome was obvious. There were 145 metabolites that significantly changed. The phenolic acids and flavonoids were the major increased categories in “R1,” and it included 4-O-glucosyl-sinapate and petunidin-3-o-(6”-o-p-coumaroyl) rutinoside. Six metabolic pathways were significantly enriched, including aminoacyl-tRNA biosynthesis, flavone, and flavonol biosynthesis. In terms of bacteria, there was no significant difference between “S1” and “R1” in the principal component analysis (PCA). Pseudomonas was the major bacterial genus in “S1” and “R1.” In addition, each of the two resources had its own predominant genus: Cellvibirio was a predominant bacterial genus in “S1” and Candidatus_competibacter was a predominant bacterial genus in “R1.” In terms of fungi, the fungal diversity and the abundance of the two tea plant resource samples could be distinguished clearly. The fungal component of “S1” was more abundant than that of “R1” at the genus level. Toxicocladosporium was the predominant fungal genus of “S1,” and Filobasidium was the predominant fungal genus of “R1.” The relative abundance of unclassified-norank-norank-Chloroplast and Penicillium were significantly different between “S1” and “R1.” Penicillium was identified as a potential biomarker. They correlated with some metabolites enriched in “S1” or “R1,” such as L-arginine and quercetin-3-o-(2”-o-rhamnosyl) rutinoside-7-o-glucoside. Overall, phenolic acids, flavonoids, and Penicillium could be functional metabolites or microorganisms that contributed to improving the resistance of wild tea plant resources to tea gray blight disease.

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“…The variability in the mycovirome suggested that the population of endophytic and/or epiphytic fungi hosting these mycoviruses was likely highly variable among genotypes in the same vineyard, and that the time span here considered (10 years from planting) was not enough for the exchange of endo/epiphytes between plants, even though they were planted closely in the same vineyard (Kraus et al ., 2019 ). Furthermore, year, location, cultivar and pre‐existing microbial communities could impact on the composition of the grapevine mycobiota (Kernaghan et al ., 2017 ; Nerva et al ., 2019b ; Cureau et al ., 2021 ).…”

Section: Resultsmentioning

confidence: 99%

Grapevine virome and production of healthy plants by somatic embryogenesis

Nuzzo

Moine

Nerva

et al. 2022

Microbial Biotechnology

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Summary Grapevine ( Vitis spp.) is a widespread fruit tree hosting many viral entities that interact with the plant modifying its responses to the environment. The production of virus‐free plants is becoming increasingly crucial for the use of grapevine as a model species in different studies. Using high‐throughput RNA sequencing, the viromes of seven mother plants grown in a germplasm collection vineyard were sequenced. In addition to the viruses and viroids already detected in grapevine, we identified 13 putative new mycoviruses. The different spread among grapevine tissues collected in vineyard, greenhouse and in vitro conditions suggested a clear distinction between viruses/viroids and mycoviruses that can successfully be exploited for their identification. Mycoviruses were absent in in vitro cultures, while plant viruses and viroids were particularly accumulated in these plantlets. Somatic embryogenesis applied to the seven mother plants was effective in the elimination of the complete virome, including mycoviruses. However, different sanitization efficiencies for viroids and grapevine pinot gris virus were observed among genotypes. The absence of mycoviruses in in vitro plantlets, associated with the absence of all viral entities in somaclones, suggested that this regeneration technique is also effective to eradicate endophytic/epiphytic fungi, resulting in gnotobiotic or pseudo‐gnotobiotic plants.

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Year, Location, and Variety Impact on Grape-Associated Mycobiota of Arkansas-Grown Wine Grapes for Wine Production

Cited by 11 publications

References 54 publications

Soil composition and rootstock genotype drive the root associated microbial communities in young grapevines

Soil composition and rootstock genotype drive the root associated microbial communities in young grapevines

Metabolome and Microbiome Signatures in the Leaves of Wild Tea Plant Resources Resistant to Pestalotiopsis theae

Grapevine virome and production of healthy plants by somatic embryogenesis

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