Grapevine virome and production of healthy plants by somatic embryogenesis

Nuzzo, Floriana; Moine, Amedeo; Nerva, Luca; Pagliarani, Chiara; Perrone, Irene; Boccacci, Paolo; Gribaudo, Ivana; Chitarra, Walter; Gambino, Giorgio

doi:10.1111/1751-7915.14011

Cited by 8 publications

(16 citation statements)

References 77 publications

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“…GPGV has previously been found by us in the vineyards of the Krasnodar Krai, Stavropol Krai, and the Republic of Crimea [35,49]. GPGV has also been found to be widely distributed in the germplasm collections of Romania, Ukraine, Bosnia, Montenegro, Serbia, Croatia, Macedonia, Portugal, Spain, Czech Republic, France, Greece, Brazil, and Italy [60,62,64,[73][74][75].…”

Section: Family: Betaflexiviridaementioning

confidence: 71%

“…It causes fleck, which is a ubiquitous disease reported in most of the world's winemaking countries [97]. The presence of GFkV has been reported in the germplasm collections of Italy [64,82], Czech Republic [26], Spain [59], Brazil [61], France [62], and Croatia [60,98]. In Russia, we have previously found GFkV on the territory of the main grape-growing zones [34,35].…”

Section: Family: Tymoviridaementioning

confidence: 90%

“…GRSPaV has been repeatedly noted to be one of the most prevalent viruses in most commercial vineyards in the world [56][57][58]. The presence of GRSPaV has also been found in the grapevine germplasm in Czech Republic [26], Spain [59], Croatia [60], Brazil [61], France [62], Tunisia [63], and Italy [56,64]. Previously, GRSPaV has been found in vineyards in southern Russia [29,34,35].…”

Section: Family: Betaflexiviridaementioning

confidence: 99%

“…GVT has been registered in the Slovakia, Czech Republic [68], Germany [69], China [70], Turkey [71], Hungary [67], and Russia [35]. GVT has been detected in the germplasm collections of Croatia [60], France [62], the USA [66], and Italy [64].…”

Section: Family: Betaflexiviridaementioning

confidence: 99%

“…In Russia, GVA was previously found in the vineyards of the Krasnodar Krai, Stavropol Krai, and the Republic of Crimea [30,34,35]. There is evidence of its presence in the grapevine germplasm in the USA [81], Spain [59], Croatia [60], Brazil [61], France [62], as well as Italy [64,82].…”

Section: Family: Betaflexiviridaementioning

confidence: 99%

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Virome of Grapevine Germplasm from the Anapa Ampelographic Collection (Russia)

Shvets

Porotikova

Sandomirsky

et al. 2022

Viruses

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Grapevine germplasm collections are unique repositories of grape cultivars; therefore, it is necessary to minimize their infection with pathogens, including viruses, and develop various programs to maintain them in a virus-free state. In our study, we examined the virome of the largest Russian grapevine germplasm collection, the Anapa Ampelographic Collection, using high-throughput sequencing of total RNAs. As a result of bioinformatics analysis and validation of its results by reverse transcription PCR (RT-PCR) and quantitative RT-PCR (RT-qPCR), we identified 20 viruses and 3 viroids in 47 libraries. All samples were infected with 2 to 12 viruses and viroids, including those that cause economically significant diseases: leafroll, fleck, and rugose wood complex. For the first time in Russia, we detected Grapevine virus B (GVB), Grapevine virus F (GVF), Grapevine asteroid mosaic-associated virus (GAMaV), Grapevine Red Globe virus (GRGV), Grapevine satellite virus (GV-Sat), Grapevine virga-like virus (GVLV), Grapevine-associated jivivirus 1 (GaJV-1) and Vitis cryptic virus (VCV). A new putative representative of the genus Umbravirus with the provisional name Grapevine umbra-like virus (GULV) was also identified in Russian grape samples.

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Section: Family: Betaflexiviridaementioning

confidence: 71%

Section: Family: Tymoviridaementioning

confidence: 90%

Section: Family: Betaflexiviridaementioning

confidence: 99%

Section: Family: Betaflexiviridaementioning

confidence: 99%

Section: Family: Betaflexiviridaementioning

confidence: 99%

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Virome of Grapevine Germplasm from the Anapa Ampelographic Collection (Russia)

Shvets

Porotikova

Sandomirsky

et al. 2022

Viruses

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Grapevine virome and production of healthy plants by somatic embryogenesis

Nuzzo

Moine

Nerva

et al. 2022

Microbial Biotechnology

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Summary Grapevine ( Vitis spp.) is a widespread fruit tree hosting many viral entities that interact with the plant modifying its responses to the environment. The production of virus‐free plants is becoming increasingly crucial for the use of grapevine as a model species in different studies. Using high‐throughput RNA sequencing, the viromes of seven mother plants grown in a germplasm collection vineyard were sequenced. In addition to the viruses and viroids already detected in grapevine, we identified 13 putative new mycoviruses. The different spread among grapevine tissues collected in vineyard, greenhouse and in vitro conditions suggested a clear distinction between viruses/viroids and mycoviruses that can successfully be exploited for their identification. Mycoviruses were absent in in vitro cultures, while plant viruses and viroids were particularly accumulated in these plantlets. Somatic embryogenesis applied to the seven mother plants was effective in the elimination of the complete virome, including mycoviruses. However, different sanitization efficiencies for viroids and grapevine pinot gris virus were observed among genotypes. The absence of mycoviruses in in vitro plantlets, associated with the absence of all viral entities in somaclones, suggested that this regeneration technique is also effective to eradicate endophytic/epiphytic fungi, resulting in gnotobiotic or pseudo‐gnotobiotic plants.

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Deciphering the interaction of bacteria inoculants with the recipient endophytic community in grapevine micropropagated plants

Vergani,

Patania,

Riva

et al. 2024

Appl Environ Microbiol

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Engineering the plant microbiome with beneficial endophytic bacteria can improve the growth, health, and productivity of the holobiont. Here, we administered two beneficial bacterial strains, Kosakonia VR04 sp. and Rhizobium GR12 sp., to micropropagated grapevine cuttings obtained via somatic embryogenesis. While both strains colonized the plant endosphere, only Rhizobium GR12 sp. increased root biomass under nutritional-deficit conditions, as supported by the plant growth promotion traits detected in its genome. Phylogenetic and co-occurrence analyses revealed that the plant native bacterial community, originally dominated by Streptococcaceae and Micrococcaceae, dramatically changed depending on the inoculation treatments, as invading strains differently affected the relative abundance and the interactions of pre-existing taxa. After 30 days of plantlets’ growth, Pantoea became a predominant taxon, and considering untreated plantlets as references, Rhizobium sp. GR12 showed a minor impact on the endophytic bacterial community. On the other hand, Kosakonia sp. VR04 caused a major change in community composition, suggesting an opportunistic colonization pattern. Overall, the results corroborate the importance of preserving the native endophytic community structure and functions during plant microbiome engineering. IMPORTANCE A better comprehension of bacterial colonization processes and outcomes could benefit the use of plant probiotics in the field. In this study, we applied two different beneficial bacteria to grapevine micropropagated plantlets and described how the inoculation of these strains impacts endophytic microbiota assembly. We showed that under nutritional deficit conditions, the response of the receiving endophytic bacterial communities to the invasion of the beneficial strains related to the manifestation of plant growth promotion effects by the inoculated invading strains. Rhizobium sp. GR12 was able to preserve the native microbiome structure despite its effective colonization, highlighting the importance of the plant-endophyte associations for the holobiont performance. Moreover, our approach showed that the use of micropropagated plantlets could be a valuable strategy to study the interplay among the plant, its native microbiota, and the invader on a wider portfolio of species besides model plants, facilitating the application of new knowledge in agriculture.

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Grapevine virome and production of healthy plants by somatic embryogenesis

Cited by 8 publications

References 77 publications

Virome of Grapevine Germplasm from the Anapa Ampelographic Collection (Russia)

Virome of Grapevine Germplasm from the Anapa Ampelographic Collection (Russia)

Grapevine virome and production of healthy plants by somatic embryogenesis

Deciphering the interaction of bacteria inoculants with the recipient endophytic community in grapevine micropropagated plants

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