Yarrowia lipolytica Lipase 2 Is Stable and Highly Active in Test Meals and Increases Fat Absorption in an Animal Model of Pancreatic Exocrine Insufficiency

Aloulou, Ahmed; Schué, Mathieu; Puccinelli, Delphine; Milano, Stéphane; Delchambre, Chantal; Leblond, Y.; Laugier, R.; Carrière, Frédéric

doi:10.1053/j.gastro.2015.08.047

Cited by 20 publications

(11 citation statements)

References 44 publications

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“…The results of this study showed that YC supplementation significantly improved the digestibility of crude fat, but its effect on the digestibility of energy or crude protein was of little significance. The digestion, absorption, and utilization of fat largely depend on trypsin digestive enzymes and emulsifiers (Aloulou et al, 2015). Additionally, studies have shown that the addition of YC can significantly increase the activity of duodenal enzymes in aged layers.…”

Section: Discussionmentioning

confidence: 99%

Yeast Culture Improves Egg Quality and Reproductive Performance of Aged Breeder Layers by Regulating Gut Microbes

et al. 2021

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This study aimed to investigate the effects of dietary yeast culture (YC) supplementation on egg production, egg quality, reproductive performance, immune functions, antioxidant capacity, and intestinal microbial structure of aged hens. A total of 224 Hy-Line Brown layers (54 weeks old) were randomly assigned to two dietary treatments. The control group was fed a basal diet and the YC group was supplemented with YC at 2.0 g/kg of their diet. Each group had seven replicates with 16 hens each. The study was conducted over a period of 8 weeks. Results indicated that YC addition had no significant effect on laying performance. However, it significantly improved egg quality and hatching rate, enhanced ileum crude fat digestibility, increased the serum parameters of lysozyme (LZM) and total antioxidation capacity (T-AOC) (P < 0.05), and reduced serum aspartate aminotransferase (AST) levels (P < 0.05). Using 16S rRNA analysis, we found that addition of YC significantly altered ileum microbial composition. Linear discriminant analysis of effect size (LEfSe) showed significant enrichment of Bacilli and Lactobacilli in the YC group. PICRUSt analysis of the ileal microbiota found that glutathione metabolism, ubiquinone, and other terpenoid-quinone biosynthesis and lipopolysaccharide biosynthesis protein pathways were highly enriched in the YC group compared with the basal diet group. In summary, the addition of YC can improve egg quality, immune functions, antioxidant capacity, reproduction efficiency, and digestive absorption by increasing the abundance of Lactobacilli and Bacilli. Furthermore, it also improves the biosynthesis of lipopolysaccharide proteins, glutathione metabolism, and the synthesis of ubiquinone and other terpenoid-quinone metabolic pathways.

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Section: Discussionmentioning

confidence: 99%

Yeast Culture Improves Egg Quality and Reproductive Performance of Aged Breeder Layers by Regulating Gut Microbes

et al. 2021

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“…The future of EPI treatment will focus on identifying recombinant microbial lipases, as already reported in the literature103 in pig models that might be good candidates to overcome difficulties in the production of porcine pancreatin and possible side effects (allergic reactions, potential risk of virus transmission to humans).…”

Section: Introductionmentioning

confidence: 99%

<p>Exocrine pancreatic insufficiency: prevalence, diagnosis, and management</p>

Capurso¹,

Traini²,

Piciucchi³

et al. 2019

CEG

135

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Exocrine pancreatic insufficiency (EPI) is a condition caused by reduced or inappropriate secretion or activity of pancreatic juice and its digestive enzymes, pancreatic lipase in particular. EPI can result in clinical manifestation and biochemical alterations causing reduced quality of life and life-threating complications. EPI is common in pancreatic disorders, where it should be suspected and actively investigated, and in many extrapancreatic conditions. There are various tests available to diagnose EPI, with indirect, noninvasive ones, such as concentration of fecal elastase being more commonly employed. Administration of pancreatic enzymes replacement therapy remains the mainstay of EPI treatment. The present review article will discuss current evidence regarding the prevalence of EPI, the available tests to diagnose it and its treatment.

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“…A good feed additive lipase candidate must be resistant to low pH values (Aloulou et al 2015). In the gizzard stomach, the pH is very acidic (pH 2-4).…”

Section: Effect Of Ph On Lipases Stabilitymentioning

confidence: 99%

Tolerance properties and growth performance assessment of Yarrowia lipolytic lipase in broilers

Wang

Yan

Zhang

et al. 2017

Journal of Applied Animal Research

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Lipases have attracted more interest for several biotechnological applications due to their unique properties, such as security, high stability at low pH values and substrate specificity. Nevertheless, no systematic studies on its effectiveness in corn-soybean when fed to broilers have been published. In this study, in vitro and in vivo assessment of a lipase from the yeast Yarrowia lipolytica for feed purpose was performed. Results showed that Y. lipolytica lipase (YLL) displayed the highest stability at low pH values when compared with other lipases from different sources. YLL stabilized with skimmed milk powder and starch maintained 55% and 48% of its initial activity after 2 h of incubation at pH 6 with bile salt and proteases, respectively. In addition, using broilers fed as substrate, YLL formulations displayed a higher hydrolysis degree after 6 h reaction in the presence of bile salt and digestive enzymes. Moreover, in vivo effect of YLL on growth performance in broilers was studied. From 1 to 42 d, it appears from this study that there is a significant (P <. 05) effect for YLL to improve feed conversion ratio. Consequently, YLL has a great potential for using as a feed supplement in the poultry industry.

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Yarrowia lipolytica Lipase 2 Is Stable and Highly Active in Test Meals and Increases Fat Absorption in an Animal Model of Pancreatic Exocrine Insufficiency

Cited by 20 publications

References 44 publications

Yeast Culture Improves Egg Quality and Reproductive Performance of Aged Breeder Layers by Regulating Gut Microbes

Yeast Culture Improves Egg Quality and Reproductive Performance of Aged Breeder Layers by Regulating Gut Microbes

<p>Exocrine pancreatic insufficiency: prevalence, diagnosis, and management</p>

Tolerance properties and growth performance assessment of Yarrowia lipolytic lipase in broilers

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