Tolerance properties and growth performance assessment of <i>Yarrowia lipolytic</i> lipase in broilers

Wang, Yaofeng; Yan, Jinyong; Zhang, Xinghua; Han, Bingnan

doi:10.1080/09712119.2017.1340298

Cited by 12 publications

(10 citation statements)

References 32 publications

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“…These effects might be explained in turn by the addition of fibrolytic enzymes to roughage in the in vitro technique, which leads to the release of reducing sugars and other products that could both enhance rumen microbial population and increase the amount of feedstuff digested (Salem et al, 2013;Mocherla and Kavitha, 2017;Sheikh et al, 2017). Wang et al (2018), in an in vitro study reported that the stimulation of rumen microbial population (nonfibrolytic and fibrolytic bacteria) by enzymes could result in a higher microbial biomass, which could then provide more total polysaccharidase activity to digest feedstuff. Blümmel and Ørskov (1993) reported that the fermentation of organic compounds produces gas as an end product, providing evidence for the strong correlation between OM digestibility and volume of gas produced.…”

Section: Cellulase and Cmcase Activities Of The Test Strains In Cultumentioning

confidence: 99%

Evaluation of cellulolytic exogenous enzyme-containing microbial inoculants as feed additives for ruminant rations composed of low-quality roughage

et al. 2020

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The supplementation of ruminant diets with exogenous cellulolytic enzymes can improve their digestibility and feeding value. The objective of this study was to determine the effect of supplementing roughage (rice straw) and concentrate with inoculants containing four fungal strains (Pleurotus ostreatus, Phanerochaete chrysosporium, Trichoderma reesei and Trichoderma viride) and four bacterial strains (Paenibacillus polymyxa, Bacillus megaterium, Bacillus circulans and Bacillus subtilis), given separately or as a mixture, as a source of exogenous cellulolytic enzymes, on basic rumen parameters in vitro, including digestibility and methane production. A batch culture trial was used to select the best supplements, and a long-term rumen simulation technique (RUSITEC) was used to evaluate the effects of P. chrysosporium, B. subtilis, and a 1 : 1 mixture of these two on dietary component digestibility and fermentation parameters. In the batch culture evaluation, there were significant increases in the organic matter (OM) digestibility, the total gas production expressed as ml/g of dry matter, the OM, the neutral detergent fibre (NDF) and the acid detergent fibre (ADF) of the supplemented rations, as compared to the control, excluding the rations supplemented with T. viride and B. circulans. In the RUSITEC, the ration supplemented with mixed inoculants showed significantly higher digestibility of crude protein, ether extract, NDF and ADF than did the ration supplemented with the P. chrysosporium and B. subtilis inoculants. It can be concluded that the simultaneous use of fungal and bacterial exogenous cellulases on rice straw roughage improves its digestibility, without negative effects on other rumen parameters.

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Section: Cellulase and Cmcase Activities Of The Test Strains In Cultumentioning

confidence: 99%

Evaluation of cellulolytic exogenous enzyme-containing microbial inoculants as feed additives for ruminant rations composed of low-quality roughage

et al. 2020

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“…According to previous studies, broiler chickens fed a diet supplemented with exogenous lipases showed an improvement in growth performance and nutrient digestibility (12,13). In contrast to these observations, a study conducted previously (14) demonstrated no significant improvement in broiler performance as a result of lipase supplementation. Similar to the studies on emulsifiers in broiler diets, the above-mentioned results are inconsistent and inconclusive.…”

Section: Introductionmentioning

confidence: 86%

Physiological Effects of a Tallow-Incorporated Diet Supplemented With an Emulsifier and Microbial Lipases on Broiler Chickens

et al. 2020

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The aim of this study was to investigate the effect of dietary emulsifiers and lipase supplementation on growth performance, blood metabolites, intestinal organ weight, gut morphology, nutrient digestibility, carcass measurements, and meat quality in broiler chickens. A total of 384, 1-day-old Ross 308 broiler chicks were randomly allocated to one of eight dietary treatments arranged in a completely randomized design with 6 replications per treatment and 8 birds per cage. Diets were corn-soybean meal-based and formulated to meet the nutritional requirements for Ross 308 specifications. Beef tallow used as the fat source in all diets. Dietary treatments were as follows, (1) positive control (PC; energy sufficient diet); (2) negative control (NC; energy deficient,−100 ME, kcal/kg); (3) NC+POL (0.1%, Polysorbate-20); (4) NC+CET (0.1%, Ceteth-20); (5) NC+POL+TLL (0.1%, Thermomyces lanuginosus lipase); (6) NC+POL+CRL (0.1%, Candida rugosa lipases); (7) NC+CET+CRL and (8) NC+LL (0.05%, Lysolecithin). Growth performances were measured weekly. One bird per pen was selected and sacrificed to collect blood, ileal digesta, jejunum sample, viscera organ weight, and meat samples on day 21 and 35. Results revealed that birds fed NC+POL+CRL diet had higher (P < 0.05) body weight, weight gain, and the improved (P < 0.05) feed efficiency compared to birds fed other low energy diets, and the effect was more prominent at the grower phase from day 21 to 35. Similarly, higher (P < 0.05) villi height and lower (P < 0.05) crypt depth commensurate with higher (P < 0.05) V:C ratio were observed with the broiler chickens fed NC+POL+CRL diet compared to broiler chickens fed NC diet on day 21 and 35. Moreover, broiler chickens fed NC+POL+CRL diet showed improved fat and energy digestibility compared NC diet counterpart on day 35. This study, therefore indicated that Polysorbate-20 together with Candida rugosa lipases had promising ability to improve growth performance of broiler chickens fed with low energy diet and curtail the growth depression without affecting blood metabolites, carcass, and visceral organs weights.

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“…The scientific literature contains a relatively small number of studies on the use of lipase (as a monocomponent) in feeds for broilers compared to the use of other exogenous enzymes (phytases, carbohydrases, proteases), and when used, lipase usually appears as a component of enzymatic complexes. However, there is a tendency in the industry to develop biotechnological processes and thereby provide lipases as feed additives (WANG et al, 2017). In combination with the use of an emulsifier, lipase could potentiate lipid digestibility and consequently the energy utilisation inherent to this nutrient.…”

Section: Introductionmentioning

confidence: 99%

Use of emulsifier and lipase in feeds for broiler chickens

Oliveira

Balbino

Silva

et al. 2019

SCA

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The objective of this study was to evaluate the effect of exogenous emulsifier and lipase in diets on performance, digestibility, and organ biometry of broiler chickens. A completely randomised design with seven treatments and seven replications was adopted. The treatments were as follows: T1 (positive control; PC): 3000, 3100, 3200, and 3250 Kcal of metabolisable energy (ME) kg-1 of diet for phases 1 to 10, 11 to 21, 22 to 31, and 32 to 37 days, respectively; T2: PC with reduction in ME of 30 Kcal kg-1 of diet; T3: PC with reduction in ME of 60 Kcal kg-1 of feed; T4 (negative control; NC): PC with reduction in ME of 90 Kcal kg-1 of feed; T5: NC with inclusion of exogenous lipase (10 000 U kg-1); T6: NC with inclusion of emulsifier (250 g t-1); and T7: NC with inclusion of lipase (10 000 U kg-1) and emulsifier (250 g t-1) in the period from 1 to 37 days of rearing. Performance characteristics (weight gain (WG), feed intake (FI), and feed conversion (FC)), carcass yield, cut yield, the relative weight of abdominal fat and organs (small intestine, liver, and pancreas), and relative intestinal length, in addition to dry matter digestibility (CDADM), ethereal extract (CDAEE), crude metabolisable energy (CMACE), and apparent metabolisable energy (AME) of the diets, were evaluated. In the initial phase, the CDAEE was higher for the PC group than for the emulsifier + lipase group. The AME determined in the final phase for the group supplemented with an emulsifier was higher by approximately 50 Kcal (EM) than the NC group. The WG of the lipase group was similar to that of the PC group. However, the groups with emulsifier and emulsifier + lipase showed a lower WG than the PC group. The additives used did not recover the FC to the same level observed in the PC group. The reduction in ME of 90 Kcal kg-1 generated a lower WG and worse FC. The use of both an emulsifier and lipase together produced results like to the PC group. The biometrics of the organs and the carcass yield and cuts were not influenced by diets. Thus, it can be concluded that the inclusion of lipase and an emulsifier improves the performance of broilers given diets with reduced energy, although it does not improve the lipid utilisation of the diets.

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Tolerance properties and growth performance assessment of Yarrowia lipolytic lipase in broilers

Cited by 12 publications

References 32 publications

Evaluation of cellulolytic exogenous enzyme-containing microbial inoculants as feed additives for ruminant rations composed of low-quality roughage

Evaluation of cellulolytic exogenous enzyme-containing microbial inoculants as feed additives for ruminant rations composed of low-quality roughage

Physiological Effects of a Tallow-Incorporated Diet Supplemented With an Emulsifier and Microbial Lipases on Broiler Chickens

Use of emulsifier and lipase in feeds for broiler chickens

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