YAP-dependent mechanotransduction is required for proliferation and migration on native-like substrate topography

Mascharak, Shamik; Benitez, Patrick L.; Proctor, Amy C.; Madl, Christopher M.; Hu, Kenneth H.; Dewi, Ruby E.; Butte, Manish J.; Heilshorn, Sarah C.

doi:10.1016/j.biomaterials.2016.11.019

Cited by 58 publications

(43 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other research has focused on designing substrates with welldefined engineered features, such as grooves, pits, and pillars (73,74). While the majority of studies reporting the impact of topography on cellular behavior have been empirical, some mechanistic insights are beginning to emerge, pointing to changes in cell-matrix adhesions and cell-cell junctions (73,75).…”

Section: Microstructurementioning

confidence: 99%

Engineering Hydrogel Microenvironments to Recapitulate the Stem Cell Niche

Madl

Heilshorn

2018

Annu. Rev. Biomed. Eng.

Self Cite

120

View full text Add to dashboard Cite

Stem cells are a powerful resource for many applications including regenerative medicine, patient-specific disease modeling, and toxicology screening. However, eliciting the desired behavior from stem cells, such as expansion in a naïve state or differentiation into a particular mature lineage, remains challenging. Drawing inspiration from the native stem cell niche, hydrogel platforms have been developed to regulate stem cell fate by controlling microenvironmental parameters including matrix mechanics, degradability, cell-adhesive ligand presentation, local microstructure, and cell-cell interactions. We survey techniques for modulating hydrogel properties and review the effects of microenvironmental parameters on maintaining stemness and controlling differentiation for a variety of stem cell types. Looking forward, we envision future hydrogel designs spanning a spectrum of complexity, ranging from simple, fully defined materials for industrial expansion of stem cells to complex, biomimetic systems for organotypic cell culture models.

show abstract

Section: Microstructurementioning

confidence: 99%

Engineering Hydrogel Microenvironments to Recapitulate the Stem Cell Niche

Madl

Heilshorn

2018

Annu. Rev. Biomed. Eng.

Self Cite

120

View full text Add to dashboard Cite

show abstract

“…Neutrophil activation and recruitment is a finely tuned process, as excessive neutrophil activity can result in significant tissue damage and increased morbidity. 2 Mechanotransduction, the ability of cells to translate mechanical forces into biochemical responses, plays an essential role in many biologic processes, including embryonic 3 and tissue development, 4 cell fate determination, 5 cell migration, 6 cell morphology, 7 cytokine activation, 8 gene expression, 5,9 and cancer cell invasion. 10 Neutrophils and other circulating leukocytes have evolved in the context of shear forces due to blood flow, consequently, they have adapted mechanosensitive molecular switches that trigger appropriate biologic responses in a shear force sensitive manner.…”

Section: Neutrophils Shear Stress and Mechanotransductionmentioning

confidence: 99%

Go with the flow: GEF-H1 mediated shear stress mechanotransduction in neutrophils

2017

View full text Add to dashboard Cite

Neutrophils in circulation experience significant shear forces due to blood flow when they tether to the vascular endothelium. Biochemical and biophysical responses of neutrophils to the physical force of flowing blood modulate their behavior and promote tissue recruitment under pro-inflammatory conditions. Neutrophil mechanotransduction responses occur through mechanisms that are not yet fully understood. In our recent work, we showed that GEF-H1, a RhoA specific guanine nucleotide exchange factor (GEF), is required to maintain neutrophil motility and migration in response to shear stress. GEF-H1 re-localizes to flottilin-rich uropods in neutrophils in response to fluid shear stress and promotes spreading and crawling on activated endothelial cells. GEF-H1 drives cellular contractility through myosin light chain (MLC) phosphorylation downstream of the Rho-ROCK signaling axis. We propose that GEF-H1-dependent cell spreading and crawling in shear stress-dependent neutrophil recruitment from the vasculature are due to the specific localization of Rho-induced contractility in the uropod.

show abstract

“…Literature data implies that curved cell bears greater in‐plane tensile forces, linking membrane curvature to increased cytoskeletal contractility, which results in activation of YAP signaling pathway. This signaling cascade plays a role in cell migration, proliferation and survival.…”

Section: Discussionmentioning

confidence: 99%

The effect of larger than cell diameter polylactic acid surface patterns on osteogenic differentiation of rat dental pulp stem cells

Alksnė

Šimoliūnas

Kalvaitytė

et al. 2018

J Biomedical Materials Res

View full text Add to dashboard Cite

Topography of the scaffold is one of the most important factors defining the quality of artificial bone. However, the production of precise micro-and nano-structured scaffolds, which is known to enhance osteogenic differentiation, is expensive and time-consuming. Meanwhile, little is known about macro-patterns (larger than cell diameter) effect on cell fate, while this kind of structures would significantly facilitate the manufacturing of artificial skeleton. Therefore, this research is focused on polylactic acid scaffold's macropattern impact on rat's dental pulp stem cells (DPSCs) morphology, proliferation, and osteogenic differentiation. For this study, two types of scaffolds were 3D printed: wavy and porous. Wavy scaffolds consisted of 188 μm wide joined threads, meaning that cells might have been curved on the filament as well as compressed in the groove. Porous scaffolds were designed to avoid groove formation and consisted of 500 μm threads, arranged in the woodpile manner, forming 300 μm diameter pores. We found that both macro-surfaces influenced DPSC morphology compared to control. As a consequence, enhanced DPSC proliferation and increased osteogenic differentiation potential was registered in cells grown on these scaffolds. Finally, our results showed that the construction of an artificial bone did not necessarily require the precise structuring of the scaffold, because both types of macrotopographic PLA scaffolds were sufficient enough to induce spontaneous DPSC osteogenic differentiation. How to cite this article: Alksne M, Simoliunas E, Kalvaityte M, Skliutas E, Rinkunaite I, Gendviliene I, Baltriukiene D, Rutkunas V, Bukelskiene V. 2019. The effect of larger than cell diameter polylactic acid surface patterns on osteogenic differentiation of rat dental pulp stem cells. J Biomed Mater Res Part A 2019:107A:174-186.

show abstract

YAP-dependent mechanotransduction is required for proliferation and migration on native-like substrate topography

Cited by 58 publications

References 55 publications

Engineering Hydrogel Microenvironments to Recapitulate the Stem Cell Niche

Engineering Hydrogel Microenvironments to Recapitulate the Stem Cell Niche

Go with the flow: GEF-H1 mediated shear stress mechanotransduction in neutrophils

The effect of larger than cell diameter polylactic acid surface patterns on osteogenic differentiation of rat dental pulp stem cells

Contact Info

Product

Resources

About