YAP balances the osteogenic and adipogenic differentiation of hPDLSCs in vitro partly through the Wnt/β-catenin signaling pathway

Jia, Linglu; Zhang, Yunpeng; Ji, Yongming; Xiong, Yixuan; Zhang, Wenjing; Yang, Wen; Xu, Xin

doi:10.1016/j.bbrc.2019.08.024

Cited by 27 publications

(31 citation statements)

References 26 publications

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“…The multilineage differentiation capacities of cells were detected as previously described [ 30 , 31 ], and cells were directed to osteogenic, adipogenic, and chondrocyte differentiation. Osteogenic induction medium contained α-MEM (Corning, USA) supplemented with 10% FBS (Gibco, USA), 10 nM dexamethasone (Dex) (Solarbio, China), 10 mM β-glycerophosphate (Solarbio, China), and 50 mg/L-ascorbic acid (Sigma, USA).…”

Section: Methodsmentioning

confidence: 99%

Downregulation of Prolactin-Induced Protein Promotes Osteogenic Differentiation of Periodontal Ligament Stem Cells

Zhang

Jia

et al. 2021

Med Sci Monit

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Background Periodontal ligament stem cells (PDLSCs) are promising seed cells for bone tissue engineering and periodontal regeneration applications. However, the mechanism underlying the osteogenic differentiation process remains largely unknown. Previous reports showed that prolactin-induced protein (PIP) was upregulated after PDLSCs osteogenic induction. However, few studies have reported on the function of PIP in osteogenic differentiation. The purpose of the present study was to investigate the effect of PIP on osteogenic differentiation of PDLSCs. Material/Methods The expression pattern of PIP during PDLSCs osteogenic differentiation was detected and the effect of each component in the osteogenic induction medium on PIP was also tested by qRT-PCR. Then, the PIP knockdown cells were established using lentivirus. The knockdown efficiency was measured and the proliferation, apoptosis, and osteogenic differentiation ability were examined to determine the functional role of PIP on PDLSCs. Results QRT-PCR showed that PIP was sustainedly upregulated during the osteogenic induction process and the phenomenon was mainly caused by the stimulation of dexamethasone in the induction medium. CCK-8 and flow cytometer showed that knocking down PIP had no influence on proliferation and apoptosis of PDLSCs. ALP staining and activity, Alizarin Red staining, and western blot analysis demonstrated PIP knockdown enhanced the osteogenic differentiation and mineralization of PDLSCs. Conclusions PIP was upregulated after osteogenic induction; however, PIP knockdown promoted PDLSCs osteogenic differentiation. PIP might be a by-product of osteogenic induction, and downregulating of PIP might be a new target in bone tissue engineering applications.

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Section: Methodsmentioning

confidence: 99%

Downregulation of Prolactin-Induced Protein Promotes Osteogenic Differentiation of Periodontal Ligament Stem Cells

Zhang

Jia

et al. 2021

Med Sci Monit

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“…Imajo et al reported that the Hippo pathway effectors, YAP and TAZ, bound to β-catenin and retained it in the cytoplasm to suppress Wnt/β-catenin signaling from 293T cells (29), whereas Jia et al found that YAP promoted the osteogenic differentiation of human periodontal ligament stem cells in vitro and inhibited adipogenic differentiation via low-density lipoprotein receptor-related protein 6 (LRP6) and dishevelled segment polarity protein 3 (dVL3)-mediated Wnt/β-catenin signaling (28). Therefore, it remains unclear whether YAP affects the proliferation and differentiation of AEcIIs via the Wnt/β-catenin pathway in BPd.…”

Section: Yap and Wnt3a Independently Promote Aeciis Proliferation Andmentioning

confidence: 99%

“…Wnt3a signals via the canonical pathway and is expressed in bronchial and alveolar epithelial cells; however, it exhibits an increased expression in fibrotic regions and the hypertrophic alveolar epithelium (27). Several studies have reported conflicting associations between YAP and the Wnt/β-catenin signaling pathway (19)(20)(21)28,29). For instance,…”

Section: Introductionmentioning

confidence: 99%

YAP and Wnt3a independently promote AECIIs proliferation and differentiation by increasing nuclear β‑catenin expression in experimental bronchopulmonary dysplasia

Jia

Huang

et al. 2020

Int J Mol Med

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Arrested alveolar development is the main pathological characteristic of neonatal bronchopulmonary dysplasia (BPD); however, a number of studies aiming to improve alveolarization have focused on alveolar epithelial cell damage and impairment. Previously, the authors reported that the Wnt signaling plays a key role in alveolar injury and repair by regulating alveolar epithelial type II cell (AECII) proliferation and differentiation. In the present study, the authors wished to investigate whether Yes-associated protein (YAP), a transcriptional coactivator in the Hippo signaling pathway, affects AECII proliferation and differentiation via the Wnt/β-catenin pathway in BPD. It was found that YAP regulated AECII proliferation and differentiation. A decreased expression of YAP, Wnt3a and nuclear β-catenin was observed in lung tissues affected by BPD. In vitro , YAP and Wnt3a overexpression in BPD promoted AECII proliferation and differentiation into AECIs by increasing the nuclear transfer of β-catenin and vice versa. The effects of a decreased Wnt3a expression in primary AECIIs in BPD were compensated by YAP overexpression, as were the effects of Wnt3a knockdown in primary AECIIs. On the whole, the findings of the present study demonstrate that YAP and Wnt3a independently promote AECII proliferation and differentiation in experimental BPD by increasing the nuclear β-catenin levels. Therefore, Wnt3a or YAP may be candidate regulatory targets for improving the outcomes of BPD.

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“…YAP1/TAZ has recently been recognized as a key regulator that promotes osteogenesis and inhibits adipogenesis through the Smad4 or β-catenin signaling pathways to maintain mouse bone homeostasis (Pan et al, 2018;Jia et al, 2019;Park et al, 2019). Pan et al (2018) elucidated the underlying mechanism by which YAP1 regulates bone homeostasis.…”

Section: Regulation Of the Osteogenic Differentiation Of Mscsmentioning

confidence: 99%

Yes-Associated Protein 1: Role and Treatment Prospects in Orthopedic Degenerative Diseases

Xie

Xiao

Tang

et al. 2020

Front. Cell Dev. Biol.

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The Hippo/yes-associated protein 1 signaling pathway is an evolutionarily conserved signaling pathway. This signaling pathway is primarily involved in the regulation of stem cell self-renewal, organ size and tissue regeneration by regulating cell proliferation, differentiation and apoptosis. It plays an important role in embryonic development and tissue organ formation. Yes-associated protein 1 (YAP1) is a key transcription factor in the Hippo signaling pathway and is negatively regulated by this pathway. Changes in YAP1 expression levels affect the occurrence and development of a variety of tumors, but the specific mechanism associated with this phenomenon has not been thoroughly studied. Recently, several studies have described the role of YAP1 in osteoarthritis (OA). Indeed, YAP1 is involved in orthopedic degenerative diseases such as osteoporosis (OP) in addition to OA. In this review, we will summarize the significance of YAP1 in orthopedic degenerative diseases and discuss the potential of the targeted modulation of YAP1 for the treatment of these diseases.

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YAP balances the osteogenic and adipogenic differentiation of hPDLSCs in vitro partly through the Wnt/β-catenin signaling pathway

Cited by 27 publications

References 26 publications

Downregulation of Prolactin-Induced Protein Promotes Osteogenic Differentiation of Periodontal Ligament Stem Cells

Downregulation of Prolactin-Induced Protein Promotes Osteogenic Differentiation of Periodontal Ligament Stem Cells

YAP and Wnt3a independently promote AECIIs proliferation and differentiation by increasing nuclear β‑catenin expression in experimental bronchopulmonary dysplasia

Yes-Associated Protein 1: Role and Treatment Prospects in Orthopedic Degenerative Diseases

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