Downregulation of Prolactin-Induced Protein Promotes Osteogenic Differentiation of Periodontal Ligament Stem Cells

Li, Yongming; Zhang, Yunpeng; Jia, Linglu; Xing, Yingjie; Zhao, Bin; Sui, Lei; Liu, Dayong; Xu, Xin

doi:10.12659/msm.930610

Cited by 6 publications

(6 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Though little is known about its exact physiological function, studies observed that prolactin-induced protein is able to bind to CD4 receptors of T lymphocytes or macrophages and the Fc fragment of immunoglobulin G, and therefore supposedly has immunomodulatory capabilities [ 51 , 52 ]. Its high expression on day 14 of osteogenic differentiation is in line with the findings of Li et al [ 53 ], where PIP upregulations were observed during the osteogenic induction of periodontal ligament stem cells (PDLSC). They concluded that the upregulation seems to be caused by dexamethasone, a glucocorticoid which is also part the StemPro ® Osteogenesis Differentiation Kit.…”

Section: Discussionsupporting

confidence: 89%

See 1 more Smart Citation

Molecular Biological Comparison of Dental Pulp- and Apical Papilla-Derived Stem Cells

Smeda

Galler

Woelflick

et al. 2022

IJMS

View full text Add to dashboard Cite

Both the dental pulp and the apical papilla represent a promising source of mesenchymal stem cells for regenerative endodontic protocols. The aim of this study was to outline molecular biological conformities and differences between dental pulp stem cells (DPSC) and stem cells from the apical papilla (SCAP). Thus, cells were isolated from the pulp and the apical papilla of an extracted molar and analyzed for mesenchymal stem cell markers as well as multi-lineage differentiation. During induced osteogenic differentiation, viability, proliferation, and wound healing assays were performed, and secreted signaling molecules were quantified by enzyme-linked immunosorbent assays (ELISA). Transcriptome-wide gene expression was profiled by microarrays and validated by quantitative reverse transcription PCR (qRT-PCR). Gene regulation was evaluated in the context of culture parameters and functionality. Both cell types expressed mesenchymal stem cell markers and were able to enter various lineages. DPSC and SCAP showed no significant differences in cell viability, proliferation, or migration; however, variations were observed in the profile of secreted molecules. Transcriptome analysis revealed the most significant gene regulation during the differentiation period, and 13 biomarkers were identified whose regulation was essential for both cell types. DPSC and SCAP share many features and their differentiation follows similar patterns. From a molecular biological perspective, both seem to be equally suitable for dental pulp tissue engineering.

show abstract

Section: Discussionsupporting

confidence: 89%

“…Dexamethasone supposedly stimulates PIP expression by a glucocorticoid receptor-dependent transcriptional activation. Moreover, they observed that a knockdown of PIP and its fibronectin-degrading properties even enhanced mineralization of PDLSC [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular Biological Comparison of Dental Pulp- and Apical Papilla-Derived Stem Cells

Smeda

Galler

Woelflick

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…Periodontitis induced by bacteria especially Gram-negative bacteria is the most prevalent chronic inflammatory disease worldwide [ 15 ]. Infections and the subsequent host immune responses have remodeled an aberrant microenvironment, which generally lead to the dysfunction of PDLSCs, which are the potential “seed cells” for periodontal regeneration applications and bone tissues engineering [ 16 , 17 ]. Interestingly, recent studies have reported that scathing PDLSCs may conduce to the perturbation of periodontal homeostasis [ 18 – 20 ].…”

Section: Discussionmentioning

confidence: 99%

EZH2 Regulates Lipopolysaccharide-Induced Periodontal Ligament Stem Cell Proliferation and Osteogenesis through TLR4/MyD88/NF-κB Pathway

Wang

Tian

Zhang

et al. 2021

Stem Cells International

View full text Add to dashboard Cite

Background. Periodontitis induced by bacteria especially Gram-negative bacteria is the most prevalent chronic inflammatory disease worldwide. Emerging evidence supported that EZH2 plays a significant role in the inflammatory response of periodontal tissues. However, little information is available regarding the underlying mechanism of EZH2 in periodontitis. This study is aimed at determining the potential role and underlying mechanism of EZH2 in periodontitis. Methods. The protein levels of EZH2, H3K27ME, p-p65, p-IKB, TLR4, MyD88, Runx2, and OCN were examined by western blot assay. Proliferation was evaluated by CCK8 assay. The levels of TNFα, IL1β, and IL6 were detected by ELISA assay. Migration was detected by wound healing assay. The distribution of p65 was detected by immunofluorescence. The formation of mineralized nodules was analyzed using alizarin red staining. Results. LPS stimulation significantly promoted EZH2 and H3K27me3 expression in primary human periodontal ligament stem cells (PDLSCs). Targeting EZH2 prevented LPS-induced upregulation of the inflammatory cytokines and inhibition of cell proliferation and migration. Furthermore, EZH2 knockdown attenuated the TLR4/MyD88/NF-κB signaling to facilitate PDLSC osteogenesis. Conclusions. Modulation of the NF-κB pathway through the inhibition of EZH2 may offer a new perspective on the treatment of chronic apical periodontitis.

show abstract

“…PIP is predominantly present within normal breast tissue, and its presence becomes lower to completely absent when the process of differentiation is lost due to the advancement of a tumor. The expression of PIP is closely connected with the differentiation of luminal epithelial cells in the mammary gland, a phenomenon that is induced by prolactin [ 22 , 23 , 24 ]. The existence of PIP under physiological and pathological conditions suggests that it is involved in many functional activities of cells.…”

Section: Pip: Structure Expression and Homeostatic Functionmentioning

confidence: 99%

Prognostic Role of Prolactin-Induced Protein (PIP) in Breast Cancer

Sauer,

Matkowski,

Bodalska

et al. 2023

Cells

View full text Add to dashboard Cite

Prolactin-inducible protein (PIP), also referred to as gross cystic disease fluid protein 15 (GCDFP-15), has been a trending topic in recent years due to its potential role as a specific marker in breast cancer. PIP binds to aquaporin-5 (AQP5), CD4, actin, fibrinogen, β-tubulin, serum albumin, hydroxyapatite, zinc α2-glycoprotein, and the Fc fragment of IgGs, and the expression of PIP has been demonstrated to be modulated by various cytokines, including IL4/13, IL1, and IL6. PIP gene expression has been extensively studied due to its captivating nature. It is influenced by various factors, with androgens, progesterone, glucocorticosteroids, prolactin, and growth hormone enhancing its expression while estrogens suppress it. The regulatory mechanisms involve important proteins such as STAT5A, STAT5B, Runx2, and androgen receptor, which collaborate to enhance PIP gene transcription and protein production. The expression level of PIP in breast cancer is dependent on the tumor stage and subtype. Higher expression is observed in early-stage tumors of the luminal A subtype, while lower expression is associated with luminal B, basal-like, and triple-negative subtypes, which have a poorer prognosis. PIP expression is also correlated with apocrine differentiation, hormone receptor positivity, and longer metastasis-free survival. PIP plays a role in supporting the immune system’s antitumor response during the early stages of breast cancer development. However, as cancer progresses, the protective role of PIP may become less effective or diminished. In this work, we summarized the clinical significance of the PIP molecule in breast cancer and its potential role as a new candidate for cell-based therapies.

show abstract

Downregulation of Prolactin-Induced Protein Promotes Osteogenic Differentiation of Periodontal Ligament Stem Cells

Cited by 6 publications

References 48 publications

Molecular Biological Comparison of Dental Pulp- and Apical Papilla-Derived Stem Cells

Molecular Biological Comparison of Dental Pulp- and Apical Papilla-Derived Stem Cells

EZH2 Regulates Lipopolysaccharide-Induced Periodontal Ligament Stem Cell Proliferation and Osteogenesis through TLR4/MyD88/NF-κB Pathway

Prognostic Role of Prolactin-Induced Protein (PIP) in Breast Cancer

Contact Info

Product

Resources

About