High contiguity de novo genome sequence assembly of 1 Trifoliate yam (Dioscorea dumetorum) using long read 2 sequencing 3 4 Abstract: The yam species Dioscorea dumetorum is one example of an orphan crop, not traded 17 internationally. Post-harvest hardening starts within 24 hours after harvesting and renders the tubers inedible.18 Genomic resources are required for trifoliate yam to improve breeding for non-hardening varieties and for 19 other traits. Here, we describe the sequencing of the D. dumetorum genome and the generation of a de novo 20 assembly together with a corresponding annotation. The two haplophases of this highly heterozygous genome 21 are separated to a large extent. The assembly represents 485 Mbp of the genome with an N50 of over 3.2 Mbp. 22 A total of 35,269 protein-encoding gene structures as well as 9,941 non-coding RNA genes were predicted and 23 functional annotations were assigned. 24 25