Xylose transport in yeast for lignocellulosic ethanol production: Current status

Sharma, Nilesh Kumar; Behera, Shuvashish; Arora, Ramesh; Kumar, Sachin; Sani, Rajesh K.

doi:10.1016/j.jbiosc.2017.10.006

Cited by 33 publications

(21 citation statements)

References 99 publications

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“…Under acidic conditions, symporters from P. guilliermondii and A. leucospermi have been detected, especially in cells cultured in YPX at pH 4.5. The highest D-xylose uptake rate observed for cells collected at 24 h for P. guilliermondii and 48 h for A. leucospermi may be associated with a low D-xylose concentration in medium, which could increase the expression of higher affinity symporters for the D-xylose substrate [16]. At longer incubation times, D-xylose from the medium had already been completely exhausted and lower transport kinetics was observed.…”

Section: Discussionmentioning

confidence: 92%

“…Other known D-xylose transporters, such as Gxf1 and Gxs1 from Candida intermedia, Xyp29, Rgt2, and Xut3 from Scheffersomyces stipitis, XylE from Escherichia coli, and An25 from Neurospora crassa [2,9], have shown limitations due to their competitive inhibition by D-glucose. To date, research in metabolic engineering has shown that S. cerevisiae, even after modification for the metabolism of pentoses, has not reported a satisfactory yield of ethanol and organic acid production [2,8,16].…”

Section: Discussionmentioning

confidence: 99%

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Prospecting for l-arabinose/d-xylose symporters from Pichia guilliermondii and Aureobasidium leucospermi

et al. 2019

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With the strong trend toward sustainable technologies, such as the gradual substitution of fossil fuel consumption, improvement in the utilization of sugars from lignocellulosic biomass appears to be an alternative for bioenergy. However, from a number of C 5 sugars, few are used in fermentative processes for ethanol production. One of the reasons is because wild-type Saccharomyces cerevisiae is unable to efficiently co-utilize hexoses and pentoses via specific transporters for each type of sugar. Thus, a system of pentose uptake that is not modulated by D-glucose is required. Here, we were able to identify the presence of sugar/H + symporters for D-xylose and L-arabinose, especially for Pichia guilliermondii, where an uptake of D-glucose via symporter was not detected. The best D-xylose uptake route in P. guilliermondii exhibited a K M of 48 mM and V MAX of 0.48 mmol h −1 g −1 at the early stationary phase (24 h). For L-arabinose, the best route of uptake exhibited a K M of 109 mM and V MAX of 0.8 mmol h −1 g −1 on log phase (12 h). The highest kinetic uptake was observed when the final pH of the medium was below 7. In general, an alkaline medium limited the expression of symporters. The results obtained in this study will help in the further investigation of these symporters through their overexpression in engineered S. cerevisiae.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Prospecting for l-arabinose/d-xylose symporters from Pichia guilliermondii and Aureobasidium leucospermi

et al. 2019

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“…As such, a number of companies and laboratories have developed very efficient recombinant yeasts that harbour native xylose-assimilating routes from bacteria, allowing co-fermentation of glucose and xylose (Ho et al, 1998). These yeasts simultaneously ferment glucose and xylose to ethanol, with more than 96% glucose and more than 90% of xylose converted into ethanol ( Alvarez et al, 2016;Ko and Lee, 2018;Sharma et al, 2018). While this is true with herbaceous lignocellulose material, when the raw material comes from wood, a series of inhibitors is generated during the pre-treatment that make the fermentation processes less efficient (Heer and Sauer, 2008;Tom as-Pej o and Olsson, 2015).…”

Section: Bioproducts From 2g Sugarsmentioning

confidence: 99%

Twenty‐first‐century chemical odyssey: fuels versus commodities and cell factories versus chemical plants

Ramos

Duque

2019

Microbial Biotechnology

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Summary The harmful effects of pollution from the massive and widespread use of fossil fuels have led various organizations and governments to search for alternative energy sources. To address this, a new energy bioprocess is being developed that utilizes non‐edible lignocellulose – the only sustainable source of organic carbon in nature. In this mini‐review, we consider the potential use of synthetic biology to develop new‐to‐nature pathways for the biosynthesis of chemicals that are currently synthesized using classical industrial approaches. The number of industrial processes based on starch or lignocellulose is still very modest. Advances in the area require the development of more efficient approaches to deconstruct plant materials, better exploitation of the catalytic potential of prokaryotes and lower eukaryotes and the identification of new and useful genes for product synthesis. Further research and progress is urgently needed in order for government and industry to achieve the major milestone of transitioning 30% of the total industry to renewable sources by 2050.

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“…Different strategies have been implemented to improve the uptake of pentose sugars including the overexpression of native and engineered HXT genes, the overexpression of heterologous xylose transporters and the evolutionary engineering of xylose-utilizing strains [6,[8][9][10][11][12]. For example, in S. cerevisiae the overexpression of hexose transporters (Hxt7 and Gal2) resulted in improved pentose consumption in the presence of glucose [13].…”

Section: Introductionmentioning

confidence: 99%

Sugar transport for enhanced xylose utilization in Ashbya gossypii

Díaz-Fernández

Muñoz‐Fernández

Martı́n

et al. 2020

Journal of Industrial Microbiology and Biotechnology

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The co-utilization of mixed (pentose/hexose) sugars constitutes a challenge for microbial fermentations. The fungus Ashbya gossypii, which is currently exploited for the industrial production of riboflavin, has been presented as an efficient biocatalyst for the production of biolipids using xylose-rich substrates. However, the utilization of xylose in A. gossypii is hindered by hexose sugars. Three A. gossypii homologs (AFL204C, AFL205C and AFL207C) of the yeast HXT genes that code for hexose transporters have been identified and characterized by gene-targeting approaches. Significant differences in the expression profile of the HXT homologs were found in response to different concentrations of sugars. More importantly, an amino acid replacement (N355V) in AFL205Cp, introduced by CRISPR/Cas9-mediated genomic edition, notably enhanced the utilization of xylose in the presence of glucose. Hence, the introduction of the afl205c-N355V allele in engineered strains of A. gossypii will further benefit the utilization of mixed sugars in this fungus.

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Xylose transport in yeast for lignocellulosic ethanol production: Current status

Cited by 33 publications

References 99 publications

Prospecting for l-arabinose/d-xylose symporters from Pichia guilliermondii and Aureobasidium leucospermi

Prospecting for l-arabinose/d-xylose symporters from Pichia guilliermondii and Aureobasidium leucospermi

Twenty‐first‐century chemical odyssey: fuels versus commodities and cell factories versus chemical plants

Sugar transport for enhanced xylose utilization in Ashbya gossypii

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