Xylastriasan A, a new cytochalasan from the fungus Xylaria striata

Abstract

Xylastriasan A (1), a new cytochalasan alkaloid with a rare 5/6/6/5/6 pentacyclic skeleton, and ergosterol (2) were isolated from the ethanol extract of fruiting bodies of the fungus Xylaria striata. Their structures were determined by analysis of their spectroscopic data. Compound 1 exhibited weak cytotoxic activity against HEPG2, B16 and A549 cell lines with IC values of 93.61, 85.61 and 91.58 μM, respectively. Ergosterol (2) potentiated pentobarbital-induced sleep by not only increasing the number of falling asleep and prolonging sleeping time but also reducing sleep latency at a dosage of 5 mg/kg.

Westalsan: A New Acetylcholine Esterase Inhibitor from the Endophytic FungusWesterdykella nigra

“…The correlations between H‐13 with H‐19 and H‐26 ( Figure 2) suggested that H‐13, H‐19, and H‐26 were oriented in the same direction, rings C/D were cis‐ fused and the oxygen bridge was oriented outward. The NOESY signals of H‐8/H‐25, and H‐18/H‐20 also confirmed the above conclusion of the relative configurations on rings C, D and E [13] …”

“…APT and HSQC spectra revealed the presence of four methylene groups at δ(C) 47.4 (C‐10), 39.6 (C‐15), 29.5 (C‐17), 43.0 (C‐20), eight methine groups at δ(C) 51.9 (C‐3), 51.7 (C‐4), 34.8 (C‐5), 36.6 (C‐8), 44.6 (C‐13), 31.3 (C‐16), 41.4 (C‐19), 24.8 (C‐22) and three quaternary carbons at δ(C) 64.0 (C‐9), 80.9 (C‐14), and 139.5 (C‐6). The above data suggested 1 to be a cytochalasan type alkaloid having a pentacyclic structure [12,13] …”

“…In the NOESY spectrum, the cross peaks of H‐3 with H‐11, H‐4 with H‐10, H‐5 with H‐8 confirmed the relative configurations on rings A and B. This is in agreement with the previous studies that reported that most cytochalasan skeletons show the same configuration, viz., cis configurations at the C‐4/C‐9 and C‐8/C‐9 ring junctions, hence the cis configurations of rings A and B [7,13–15] . The correlations between H‐13 with H‐19 and H‐26 ( Figure 2) suggested that H‐13, H‐19, and H‐26 were oriented in the same direction, rings C/D were cis‐ fused and the oxygen bridge was oriented outward.…”

Investigation of α/γ hybrid peptide self‐assembled structures with antimicrobial and antibiofilm properties

“…The experiments were performed in triplicate, and the data were averaged. To study the effect of peptides P1 and P2 on bacterial membrane integrity, 4′,6‐diamidino‐2‐phenylindole dihydrochloride (DAPI) and propidium iodide (PI) were used . Briefly, the bacterial cultures at mid‐log phase were incubated with peptide P1 and P2 at 250 μg/mL concentration against E. coli as well as 400 μg/mL for K .…”

Anti-aging Activity of Xylaria striata in Drosophila melanogaster

“…It was used as folk medicine in China [7]. After finding its diverse biological activities in the preliminary screening of large numbers of edible and medicinal mushrooms, our research group started a systematic study on Xylaria striata from cultivation method to chemical components since 2013 [8][9]. These research results clarified its effect of anti-animal and plant pathogens [10], anti-tumor [11], and promoting sleep [12].…”