Xylanases of Cellulomonas flavigena: expression, biochemical characterization, and biotechnological potential

Lisov, A. V.; Belova, O. V.; Lisova, Zoya A.; Vinokurova, N. G.; Nagel, Alexey; Andreeva-Kovalevskaya, Zhanna I.; Budarina, Zhanna I.; Nagornykh, Maxim; Zakharova, Marina; Shadrin, Andrey M.; Solonin, Alexander S.; Leontievsky, A. A.

doi:10.1186/s13568-016-0308-7

Cited by 19 publications

(14 citation statements)

References 31 publications

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“…Their (hemi)cellulolytic system was studied by growth in defined substrates, such as xylan or cellulose, revealing a complex array of enzymatic activities involved in polysaccharide utilization (Ponce-Noyola and De La Torre 2001;Amaya-Delgado et al 2006). Some individual GHs of both species have also been characterized, confirming their activity (Din et al 1990;Mayorga-Reyes et al 2002;Santiago-Hern andez et al 2007;Gao and Wakarchuk 2014;Lisov et al 2017;Yang et al 2015).…”

Section: Introductionmentioning

confidence: 90%

Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates

et al. 2018

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Aims Lignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value‐added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual biomass for the formulation of cost‐efficient enzymatic cocktails. Methods and Results We identified 205 potential CAZymes in the genome of Cellulomonas sp. B6, 91 of which were glycoside hydrolases (GH). By secretome analysis of supernatants from cultures in either extruded wheat straw (EWS), grinded sugar cane straw (SCR) or carboxymethylcellulose (CMC), we identified which proteins played a role in lignocellulose deconstruction. Growth on CMC resulted in the secretion of two exoglucanases (GH6 and GH48) and two GH10 xylanases, while growth on SCR or EWS resulted in the identification of a diversity of CAZymes. From the 32 GHs predicted to be secreted, 22 were identified in supernatants from EWS and/or SCR cultures, including endo‐ and exoglucanases, xylanases, a xyloglucanase, an arabinofuranosidase/β‐xylosidase, a β‐glucosidase and an AA10. Surprisingly, among the xylanases, seven were GH10. Conclusions Growth of Cellulomonas sp. B6 on lignocellulosic biomass induced the secretion of a diverse repertoire of CAZymes. Significance and Impact of the Study Cellulomonas sp. B6 could serve as a source of lignocellulose‐degrading enzymes applicable to bioprocessing and biotechnological industries.

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Section: Introductionmentioning

confidence: 90%

Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates

et al. 2018

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“…4). Lisov et al (2017) found that the temperature optima for xylanases from Cellulomonas flavigena are 40 °C by CFXyl1 and 50 °C for CFXyl4, CFXyl3, and CFXyl2. Xylanases are stable below 60 °C and degrade rapidly at 65 to 70 °C (Amaya et al 2010).…”

Section: Enzyme Activity Assaysmentioning

confidence: 99%

“…The optimal pH of xylanase from C. flavigena was determined by Amaya et al (2010) to be 6.5 and 5.7 in C. fimi by Chen et al (2012). Xylanases show the highest stability at pH 8 to 10 (Lisov et al 2017). Table 1 shows the content of cellulose hemicelluloses and lignin content in the agricultural substrates.…”

Section: Enzyme Activity Assaysmentioning

confidence: 99%

“…Sugar content released from corn stover was 18.903+0.6506 mM/L when treated with crude xylanase as compared to sugar content released by treatment with CMCase (14.442+0.724 mM/L). Lisov et al (2017) hydrolyzed rye, wheat, and oat with the xylanases isolated from Cellulomonas flavigena and obtained the highest yield from rye (approximately 1.3mM/L). Bacillus sp.…”

Section: Enzyme Activity Assaysmentioning

confidence: 99%

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Cellulomonas sp. Isolated from Termite Gut for Saccharification and Fermentation of Agricultural Biomass

et al. 2017

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Biofuel is an important alternative source of fuel, as many countries are looking to decrease their dependence on fossil fuels. One of the critical steps in biofuel production is the conversion of lignocelluloses to fermentable sugars, and there is need for cheaper and more efficient enzymatic strategies. Consequently, lignocellulase genes from various organisms have been explored. Termites possess varied sets of efficient micro-scale lignocellulose degrading systems. In this study, bacteria that degraded cellulose and xylan were isolated from termite gastrointestinal tract. The isolate was identified as Cellulomonas sp. by 16S rRNA gene sequencing. The bacterial enzymes cellulase and xylanase showed the highest activity at 50 °C and pH 8.0. The agricultural substrates were hydrolyzed by cellulases and xylanases, and more sugar was released from corn stover (18.903+0.65 mM/L) than from rice straw or cotton stalk. After direct hydrolysis and fermentation of agricultural substrates, ethanol (0.425+0.035 g/L) and lactate (0.772+0.075 g/L) were the major end products. Thus, termite gut bacteria can efficiently hydrolyze hemicellulose and cellulose, and these bacteria also have the potential to convert these fermentable sugars into valuable secondary metabolites.

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“…Diversos gêneros bacterianos já foram reportados como produtores de xilanases, como Bacillus, Cellulomonas, Micrococcus, Staphylococcus, Paenibacillus, Arthrobacter, Microbacterium, Pseudoxanthomonas eRhodothermus (Chakdar H et al, 2016). Algumas espécies, como Bacillusarseniciselenatis (Kamble RD, Jadhav AR, 2012), Cellulomonasflavigena (Lisov AV et al, 2017),…”

Section: Xilanasesunclassified

Production of thermostable multiple enzymes from Bacillus amyloliquefaciens KUB29

Devaraj

Aathika

Periyasamy

et al. 2018

Natural Product Research

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A strain of Bacillus amyloliquefaciens KUB29 was identified by 16S ribosomal RNA sequencing (Genbank: MF772779.1). Production of thermostable protease, amylase and lipase were done by the isolated strain. The produced enzymes were partially purified by ammonium precipitation followed by dialysis process. Protease and lipase enzymes are effectively used in bio-oil extraction from proteinaceous sample followed by transesterification to produce methyl ester. Amylase enzyme is widely used in food and laundry industry. The produced enzymes are active at thermophilic condition of 55 °C. Use of these enzymes in biofuel production process will make the process cleaner and greener.

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Xylanases of Cellulomonas flavigena: expression, biochemical characterization, and biotechnological potential

Cited by 19 publications

References 31 publications

Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates

Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates

Cellulomonas sp. Isolated from Termite Gut for Saccharification and Fermentation of Agricultural Biomass

Production of thermostable multiple enzymes from Bacillus amyloliquefaciens KUB29

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