Xylanase from Acinetobacter pittii MASK 25 and developed magnetic cross-linked xylanase aggregate produce predominantly xylopentose and xylohexose from agro biomass

Purohit, Anjali; Kumar, Shushil; Chownk, Manisha; Sangwan, Rajender S.; Yadav, Sudesh Kumar

doi:10.1016/j.biortech.2017.08.034

Cited by 37 publications

(13 citation statements)

References 41 publications

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“…The extracellular protein and endoxylanase activity on the other hand still increased after 14 h, thus ruling out any correlation between the growth and the extracellular protein production and the endoxylanase activity. Purohit and coworkers (2017) also observed that the maximum enzyme activity was observed after 24 h when the maximum growth for Acinetobacter putii MASK25 was achieved [ 29 ]. It was also reported by Shulami and coworkers (2014) that Geobacillus stearothermophilus produced xylanase during the late exponential phase, where the cells could survive the nutrient limiting conditions, and produced more xylanase to hydrolyze the xylan and generate more sugar [ 15 ].…”

Section: Resultsmentioning

confidence: 99%

Thermostable Xylanase Production by Geobacillus sp. Strain DUSELR13, and Its Application in Ethanol Production with Lignocellulosic Biomass

2018

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The aim of the current study was to optimize the production of xylanase, and its application for ethanol production using the lignocellulosic biomass. A highly thermostable crude xylanase was obtained from the Geobacillus sp. strain DUSELR13 isolated from the deep biosphere of Homestake gold mine, Lead, SD. Geobacillus sp. strain DUSELR13 produced 6 U/mL of the xylanase with the beechwood xylan. The xylanase production was improved following the optimization studies, with one factor at a time approach, from 6 U/mL to 19.8 U/mL with xylan. The statistical optimization with response surface methodology further increased the production to 31 U/mL. The characterization studies revealed that the crude xylanase complex had an optimum pH of 7.0, with a broad pH range of 5.0–9.0, and an optimum temperature of 75 °C. The ~45 kDa xylanase protein was highly thermostable with t1/2 of 48, 38, and 13 days at 50, 60, and 70 °C, respectively. The xylanase activity increased with the addition of Cu+2, Zn+2, K+, and Fe+2 at 1 mM concentration, and Ca+2, Zn+2, Mg+2, and Na+ at 10 mM concentration. The comparative analysis of the crude xylanase against its commercial counterpart Novozymes Cellic HTec and Dupont, Accellerase XY, showed that it performed better at higher temperature, hydrolyzing 65.4% of the beechwood at 75 °C. The DUSEL R13 showed the mettle to hydrolyze, and utilize the pretreated, and untreated lignocellulosic biomass: prairie cord grass (PCG), and corn stover (CS) as the substrate, and gave a maximum yield of 20.5 U/mL with the untreated PCG. When grown in co-culture with Geobacillus thermoglucosidasius, it produced 3.53 and 3.72 g/L ethanol, respectively with PCG, and CS. With these characteristics the xylanase under study could be an industrial success for the high temperature bioprocesses.

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Section: Resultsmentioning

confidence: 99%

Thermostable Xylanase Production by Geobacillus sp. Strain DUSELR13, and Its Application in Ethanol Production with Lignocellulosic Biomass

2018

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“…Nonetheless, Reddy and Krishnan (2016a), da Silva Menezes et al ( 2018), Palaniappan et al (2017) and Purohit et al (2017) reported similar yield values using corncob, 670-720 mg/g, though the production times vary greatly (from 1 to 30 h), emphasizing the importance of using suitable residues and xylanase sources. Moreover, it is noteworthy that the majority of the processes include a first step of residue pretreatment, therefore the Y XOS/xylan values showed in Table 1 do not represent the overall production process yield (Faryar et al, 2015;Reddy and Krishnan, 2016a;Mathew et al, 2018;Palaniappan et al, 2017;Rajagopalan et al, 2017;Salas-Veizaga et al, 2017;Seesuriyachan et al, 2017;Liu et al, 2018;Singh et al, 2018), except for the ones reported by Amorim et al (2018Amorim et al ( , 2019b, Purohit et al (2017) andda Silva Menezes et al (2018). Purohit et al (2017) andda Silva Menezes et al (2018) used direct enzymatic hydrolysis of rice straw and corn cobs, and rice husk, respectively.…”

Section: Future Trends On Xos Productionmentioning

confidence: 97%

“…Table 1 summarizes the yields of XOS produced (mg) per gram of xylan, Y XOS/xylan (mg/g), that have been recently reported for enzymatic hydrolysis of agro-residues with and without pretreatment. Purohit et al (2017) reported both the highest yield and the lowest production time, using a magnetic cross-linked xylanase aggregate developed from Acinetobacter pittii MASK 25 xylanase to hydrolyze milled rice straw. This study clearly reinforces the importance of sophisticated genetic strategies to increase the process efficiency.…”

Section: Future Trends On Xos Productionmentioning

confidence: 99%

From lignocellulosic residues to market: Production and commercial potential of xylooligosaccharides

Amorim

Silvério

Prather

et al. 2019

Biotechnology Advances

205

108

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The updated definition of prebiotic expands the range of potential applications in which emerging xylooligosaccharides (XOS) can be used. It has been demonstrated that XOS exhibit prebiotic effects at lower amounts compared to others, making them competitively priced prebiotics. As a result, the industry is focused on developing alternative approaches to improve processes efficiency that can meet the increasing demand while reducing costs. Recent advances have been made towards greener and more efficient processes, by applying process integration strategies to produce XOS from costless lignocellulosic residues and using genetic engineering to create microorganisms that convert these residues to XOS. In addition, collecting more in vivo data on their performance will be key to achieve regulatory claims, greatly increasing XOS commercial value.

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“…Process improvement through sequential optimization of culture condition for enhanced production of xylanases has been reported from wild isolated Acinetobacter sp (Lo et al, 2010). Moreover, Purohit et al (2017) identified and characterized a new bacterial strain Acinetobacter pittii MASK25 producing xylanase.…”

Section: Issn: 2320-5407mentioning

confidence: 99%

SEMI-SIMULTANOUS SACCHARIFICATION AND FERMENTATION OF Opuntia ficus-indica CLADODE FOR BIOETHANOL PRODUCTION USING WILD STRAIN.

Domínguez¹,

Sucre²,

Buenfil³

2018

IJAR

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Xylanase from Acinetobacter pittii MASK 25 and developed magnetic cross-linked xylanase aggregate produce predominantly xylopentose and xylohexose from agro biomass

Cited by 37 publications

References 41 publications

Thermostable Xylanase Production by Geobacillus sp. Strain DUSELR13, and Its Application in Ethanol Production with Lignocellulosic Biomass

Thermostable Xylanase Production by Geobacillus sp. Strain DUSELR13, and Its Application in Ethanol Production with Lignocellulosic Biomass

From lignocellulosic residues to market: Production and commercial potential of xylooligosaccharides

SEMI-SIMULTANOUS SACCHARIFICATION AND FERMENTATION OF Opuntia ficus-indica CLADODE FOR BIOETHANOL PRODUCTION USING WILD STRAIN.

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