Xylanase and β-glucosidase production by Aspergillus fumigatus using commercial and lignocellulosic substrates submitted to chemical pre-treatments

Rodrigues, Patrísia de Oliveira; Santos, Beatriz Vieira dos; Costa, Lílian Lúcia; Henrique, Mariana Alves; Pasquini, Daniel; Baffi, Milla Alves

doi:10.1016/j.indcrop.2016.10.055

Cited by 30 publications

(5 citation statements)

References 34 publications

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“…In addition, BS also induced the production of endo-and exoglucanases, enzymes which were not detected by dos Santos et al (2015). One explanation for this result may be the high lignin content in SCB (around 29Á5% as observed by Rodrigues et al 2017) in comparison to BS (21Á72%), which could reduce the induction of cellulolytic enzymes. Furthermore, in the SSF carried out with (NH 4 ) 2 SO 4 and only BS, the production of b-glucosidase was low (36Á77 U g À1 ), suggesting once more that peptone could potentiate the production of this enzyme (Table 4).…”

Section: Discussionmentioning

confidence: 86%

Biomass sorghum as a novel substrate in solid-state fermentation for the production of hemicellulases and cellulases byAspergillus nigerandA. fumigatus

et al. 2018

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Section: Discussionmentioning

confidence: 86%

Biomass sorghum as a novel substrate in solid-state fermentation for the production of hemicellulases and cellulases byAspergillus nigerandA. fumigatus

et al. 2018

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“…The contents of cellulose, hemicellulose and lignin in the samples before and after pretreatment were calculated by following the National Renewable Energy Laboratory (NREL) method as described in Sluiter et al [21]. The surface morphology of pretreated and enzyme-hydrolyzed samples was imaged using NanoSEM 490 SEM (FEI.Co, USA) with an accelerating voltage of 15 kV at magnifications of 1000 [22]. The crystalline phases of pretreated and hydrolyzed straws were characterized by X-ray diffractogram (XRD, Bruker D5005, Karlsruhe, Germany).…”

Section: Methodsmentioning

confidence: 99%

Pretreatment of sweet sorghum straw and its enzymatic digestion: insight into the structural changes and visualization of hydrolysis process

Dong

Wang

et al. 2019

Biotechnol Biofuels

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BackgroundThe efficient utilization of lignocellulosic biomass for biofuel production has received increasing attention. Previous studies have investigated the pretreatment process of biomass, but the detailed enzymatic hydrolysis process of pretreated biomass remains largely unclear. Thus, this study investigated the pretreatment efficiency of dilute alkali, acid, hydrogen peroxide and its ultimate effects on enzymatic hydrolysis. Furthermore, to better understand the enzymatic digestion process of alkali-pretreated sweet sorghum straw (SSS), multimodal microscopy techniques were used to visualize the enzymatic hydrolysis process.ResultAfter pretreatment with alkali, an enzymatic hydrolysis efficiency of 86.44% was obtained, which increased by 99.54% compared to the untreated straw (43.23%). The FTIR, XRD and SEM characterization revealed a sequence of microstructural changes occurring in plant cell walls after pretreatment, including the destruction of lignin–polysaccharide interactions, the increase of porosity and crystallinity, and reduction of recalcitrance. During the course of hydrolysis, the cellulase dissolved the cell walls in the same manner and the digestion firstly occurred from the middle of cell walls and then toward the cell wall corners. The CLSM coupled with fluorescent labeling demonstrated that the sclerenchyma cells and vascular bundles in natural SSS were highly lignified, which caused the nonproductive bindings of cellulase on lignin. However, the efficient delignification significantly increased the accessibility and digestibility of cellulase to biomass, thereby improving the saccharification efficiency.ConclusionThis work will be helpful in investigating the biomass pretreatment and its structural characterization. In addition, the visualization results of the enzymatic hydrolysis process of pretreated lignocellulose could be used for guidance to explore the lignocellulosic biomass processing and large-scale biofuel production.

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“…Interestingly, selection of alternative lower cost agriculture residues to treat by solid-state fermentation plays a prominent role in the green eco-friendly system, since it benefits in two ways. One of them, providing a possibility to the production of several microbial enzymes with an elevated yield and a lower cost in comparison to the most used culture media (de Cassia Pereira et al 2015 ; Rodrigues et al 2017 ; Singhania et al 2010 ). The other way, the fiber characteristics in these alternative substrates would be improved as a result of the synergistic action of microbial enzymes along with the hydrolyzing COW, thus yielding maximum heating capacity yield.…”

Section: Resultsmentioning

confidence: 99%

A novel pressed coal from citrus and cooking oil wastes using fungi

Hasanin

Hashem

Hashish

et al. 2022

Bioresour. Bioprocess.

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Nowadays renewable energy with low prices is a global target that has taken the attention to compare alternatives energy sources with fossil fuels. Therefore, this study was established to find suitable and sustainable alternative low-cost fuels source. Cooking oil waste (COW) was mixed with non-pretreated citrus tree fibers (CTF) (0.5 mL to 1 g ratio) and pressed to formulate coal (CTF/COW). Otherwise, this mixture was subjected to in situ fungal pretreated using Aspergillus flavus isolate to simplify the mixture composition and pressed to offer in a usable form with enhancing their heating value for the first time. CTF/COW was characterized using attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR), scanning electron microscope (SEM) and thermal analysis (TGA) before and after treatment. The fungal isolate was observed with enzyme productivity and activity of CMCase, avicelase, xylanase, mannanase, α-glucosidase, β-glucosidase, lignin peroxidase and lipase according to enzyme assays and the chemical compositions of CTF before and after fungal treatment, where the best PH for enzymes extraction was between 5 and 7. The fungal enzymes increased the heating value by about two and half folds in comparison with non-pretreated coal. Moreover, the calorific value of tCTF/COW was 43,422 kJ/kg, which was higher than CTF recorded 18,214 kJ/kg and COW recorded 39,823 kJ/kg. Our result suggests that fungal treatment of the mixture of citrus trees and cooking oil waste presents as a promising low-cost and eco-friendly coal. Graphical Abstract

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Xylanase and β-glucosidase production by Aspergillus fumigatus using commercial and lignocellulosic substrates submitted to chemical pre-treatments

Cited by 30 publications

References 34 publications

Biomass sorghum as a novel substrate in solid-state fermentation for the production of hemicellulases and cellulases byAspergillus nigerandA. fumigatus

Biomass sorghum as a novel substrate in solid-state fermentation for the production of hemicellulases and cellulases byAspergillus nigerandA. fumigatus

Pretreatment of sweet sorghum straw and its enzymatic digestion: insight into the structural changes and visualization of hydrolysis process

A novel pressed coal from citrus and cooking oil wastes using fungi

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