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Jangiam, Witawat; Loprasert, Suvit; Tungpradabkul, Sumalee

doi:10.2306/scienceasia1513-1874.2008.34.023

Cited by 3 publications

(1 citation statement)

References 15 publications

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“…Furthermore, the expression of most of the genes encoding sigma factors was repressed in the intracellular bacteria. Under oxidative stress, rpoS regulates oxyR and katG dpsA operons [29]. As expected, we observed down regulation of oxyR and katG dpsA expression at 1 and 2 h post-infection.…”

Section: Resultssupporting

confidence: 86%

Burkholderia pseudomallei transcriptional adaptation in macrophages

2012

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BackgroundBurkholderia pseudomallei is a facultative intracellular pathogen of phagocytic and non-phagocytic cells. How the bacterium interacts with host macrophage cells is still not well understood and is critical to appreciate the strategies used by this bacterium to survive and how intracellular survival leads to disease manifestation.ResultsHere we report the expression profile of intracellular B. pseudomallei following infection of human macrophage-like U937 cells. During intracellular growth over the 6 h infection period, approximately 22 % of the B. pseudomallei genome showed significant transcriptional adaptation. B. pseudomallei adapted rapidly to the intracellular environment by down-regulating numerous genes involved in metabolism, cell envelope, motility, replication, amino acid and ion transport system and regulatory function pathways. Reduced expression in catabolic and housekeeping genes suggested lower energy requirement and growth arrest during macrophage infection, while expression of genes encoding anaerobic metabolism functions were up regulated. However, whilst the type VI secretion system was up regulated, expression of many known virulence factors was not significantly modulated over the 6hours of infection.ConclusionsThe transcriptome profile described here provides the first comprehensive view of how B. pseudomallei survives within host cells and will help identify potential virulence factors and proteins that are important for the survival and growth of B. pseudomallei within human cells.

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Section: Resultssupporting

confidence: 86%

Burkholderia pseudomallei transcriptional adaptation in macrophages

2012

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Role ofBurkholderia pseudomalleiSigma N2 in Amino Acids Utilization and in Regulation of Catalase E Expression at the Transcriptional Level

Diep

Phuong

Hlaing

et al. 2015

International Journal of Bacteriology

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Burkholderia pseudomallei is the causative agent of melioidosis. The complete genome sequences of this pathogen have been revealed, which explain some pathogenic mechanisms. In various hostile conditions, for example, during nitrogen and amino acid starvation, bacteria can utilize alternative sigma factors such as RpoS and RpoN to modulate genes expression for their adaptation and survival. In this study, we demonstrate that mutagenesis of rpoN2, which lies on chromosome 2 of B. pseudomallei and encodes a homologue of the sigma factor RpoN, did not alter nitrogen and amino acid utilization of the bacterium. However, introduction of B. pseudomallei rpoN2 into E. coli strain deficient for rpoN restored the ability to utilize amino acids. Moreover, comparative partial proteomic analysis of the B. pseudomallei wild type and its rpoN2 isogenic mutant was performed to elucidate its amino acids utilization property which was comparable to its function found in the complementation assay. By contrast, the rpoN2 mutant exhibited decreased katE expression at the transcriptional and translational levels. Our finding indicates that B. pseudomallei RpoN2 is involved in a specific function in the regulation of catalase E expression.

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Burkholderia pseudomalleiAdaptation for Survival in Stressful Conditions

Duangurai

Indrawattana

Pumirat

2018

BioMed Research International

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Burkholderia pseudomallei is a Gram-negative bacterium that causes melioidosis, which can be fatal in humans. Melioidosis is prevalent in the tropical regions of Southeast Asia and Northern Australia. Ecological data have shown that this bacterium can survive as a free-living organism in environmental niches, such as soil and water, as well as a parasite living in host organisms, such as ameba, plants, fungi, and animals. This review provides an overview of the survival and adaptation of B. pseudomallei to stressful conditions induced by hostile environmental factors, such as salinity, oxidation, and iron levels. The adaptation of B. pseudomallei in host cells is also reviewed. The adaptive survival mechanisms of this pathogen mainly involve modulation of gene and protein expression, which could cause alterations in the bacteria's cell membrane, metabolism, and virulence. Understanding the adaptations of this organism to environmental factors provides important insights into the survival and pathogenesis of B. pseudomallei, which may lead to the development of novel strategies for the control, prevention, and treatment of melioidosis in the future.

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Cited by 3 publications

References 15 publications

Burkholderia pseudomallei transcriptional adaptation in macrophages

Burkholderia pseudomallei transcriptional adaptation in macrophages

Role ofBurkholderia pseudomalleiSigma N2 in Amino Acids Utilization and in Regulation of Catalase E Expression at the Transcriptional Level

Burkholderia pseudomalleiAdaptation for Survival in Stressful Conditions

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