Xuanfei Baidu formula alleviates impaired mitochondrial dynamics and activated NLRP3 inflammasome by repressing NF-κB and MAPK pathways in LPS-induced ALI and inflammation models

Li, Zhenhao; Pan, Haitao; Yang, Jihong; Chen, Dongjie; Wang, Yu; Zhang, Han; Cheng, Yiyu

doi:10.1016/j.phymed.2022.154545

Cited by 42 publications

(20 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A mouse model of pulmonary fibrosis bleomycin treated with Xuanfei Baidu decoction showed that this medicine could effectively protect the alveolar structure, reduce the cellular changes of lung, improve the infiltration of inflammatory cells, and inhibit the thickening of the blood vessel wall and collagen deposition; furthermore, after network pharmacology and protein-protein interaction analysis, we found that the above effects were related to inhibition of the activation of interleukin (IL)-6/STAT3 signaling pathway, preventing the differentiation of M2 macrophages, and downregulating the overexpression of inflammatory factors such as IL-6 and transforming growth factor (TGF)-β [14] . The model of acute lung injury induced by lipopolysaccharide LPS showed that Xuanfei Baidu decoction can improve the pathological changes of lung tissue in mice, downregulate the levels of inflammatory factors IL-6, tumor necrosis factor (TNF)-α, and IL-1β in serum and alveolar lavage fluid of mice, and also inhibit the infiltration of macrophages and neutrophils [15][16][17] . Its mechanism is related to the inhibition of programmed death (PD)-1/IL17A pathways [15] and the polarization of proinflammatory macrophages by increasing mitochondrial fusion and inhibiting the activation of NLRP3 inflammatory bodies mediated by nuclear factor kappa-B (NF-κB) and mitogen-activated protein kinase (MAPK) pathways [16][17] .…”

Section: Discussionmentioning

confidence: 99%

“…The model of acute lung injury induced by lipopolysaccharide LPS showed that Xuanfei Baidu decoction can improve the pathological changes of lung tissue in mice, downregulate the levels of inflammatory factors IL-6, tumor necrosis factor (TNF)-α, and IL-1β in serum and alveolar lavage fluid of mice, and also inhibit the infiltration of macrophages and neutrophils [15][16][17] . Its mechanism is related to the inhibition of programmed death (PD)-1/IL17A pathways [15] and the polarization of proinflammatory macrophages by increasing mitochondrial fusion and inhibiting the activation of NLRP3 inflammatory bodies mediated by nuclear factor kappa-B (NF-κB) and mitogen-activated protein kinase (MAPK) pathways [16][17] . In addition, Xuanfei Baidu decoction can also improve weight loss, spleen index, disease activity index, TNF-α level, and colon tissue injury of mice with acute colitis.…”

Section: Discussionmentioning

confidence: 99%

“…The mechanism may be related to the PI3K-Akt, HIF-1, and TNF signaling pathways. Moreover, more than 60 ingredients and metabolites were detected in plasma of animals that were intragastrically administered with Xuanfei Baidu decoction, and more than 50 ingredients and metabolites were distributed in lung tissues, among which the active ingredients polydatin, isoliquiritin, ephedrine, kaempferol, atractylenolide I, and verbascoside are closely related to the negative regulation of inflammation-induced macrophage activation and the reduction of lung inflammation [16] .…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Expert Consensus on the clinical application of Xuanfei Baidu granule

Liu¹,

Zhang²,

Wang³

et al. 2023

Acupuncture and Herbal Medicine

View full text Add to dashboard Cite

Xuanfei Baidu granule is an effective prescription for COVID-19, which is modified from classical famous prescriptions. In order to further standardize clinician’s rational use of Xuanfei Baidu granule, enhance clinical efficacy, improve rational medication level, and let it play a more significant role, the frontline clinical experts are invited for discussion on the major functions, usage, adverse reactions, and considerations of Xuanfei Baidu granule to form the consensus. The consensus is that: Xuanfei Baidu granule has the efficacy of promoting the lung and resolving dampness, clearing heat and removing pathogeneses, purging the lung and depoisoning; it can be used for the treatment of patients with mild, moderate, and heavy COVID-19 and other common upper respiratory tract infectious diseases such as influenza; acute tracheitis and bronchitis and community-acquired lower respiratory tract infectious diseases all belong to damp-toxin constraint in the lungs, Xuanfei Baidu granule can relieve cough, expectoration, and other symptoms caused by an acute exacerbation of the chronic obstructive pulmonary disease, with definite clinical efficacy and good safety. According to the principles of evidence-based medicine, this expert consensus will be updated regularly.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Expert Consensus on the clinical application of Xuanfei Baidu granule

Liu¹,

Zhang²,

Wang³

et al. 2023

Acupuncture and Herbal Medicine

View full text Add to dashboard Cite

show abstract

“…Once NF-κB is activated, the secretions of a large number of inflammatory cytokines will aggravate secondary damage and induce cell apoptosis in sepsis ( 13 , 14 ). In many disease models, inhibition of NF-κB activation also has been shown to effectively reduce cell apoptosis ( 8 , 13 , 15 – 17 ). Therefore, NF-κB is considered as an important therapeutic target for sepsis and inflammatory diseases.…”

Section: Introductionmentioning

confidence: 99%

Ginkgolic acid promotes inflammation and macrophage apoptosis via SUMOylation and NF-κB pathways in sepsis

et al. 2023

View full text Add to dashboard Cite

BackgroundExcessive inflammation and increased apoptosis of macrophages contribute to organ damage and poor prognosis of sepsis. Ginkgolic acid (GA) is a natural constituent extracted from the leaves of Ginkgo biloba, that can regulate inflammation and apoptosis. The present study aims to investigate the potential effect of GA in treating sepsis and its possible mechanisms.Materials and methodsHere, a classic septic mice model and a lipopolysaccharide (LPS)-induced RAW 264.7 inflammation model were established. Cytokines in serum and culture supernatant were detected by ELISA, and the mRNA levels of them were examined by PCR. Hematoxylin and eosin (H&E) staining was performed to determine histopathological changes in liver, lung and kidney. Bacterial burden in the blood, peritoneal lavage fluids (PLFs) and organs were observed on Luria-Bertani agar medium. Flow cytometry and western blotting was used to detect apoptosis and the expression level of apoptosis related molecules, respectively. Moreover, the levels of SUMOylation were detected by western blotting. The activity of NF-κB p65 was assessed by immunofluorescence staining and western blotting.ResultsThe result showed that GA promoted inflammatory responses, reduced bacterial clearance, aggravated organ damage, and increased mortality in septic mice. GA increased apoptosis in peritoneal macrophages (PMs) and RAW 264.7 cells. Meanwhile, GA inhibited SUMOylation and increased the nuclear translocation of NF-κB p65 as well as its phosphorylation level.ConclusionCollectively, GA promotes inflammation and macrophage apoptosis in sepsis, which may be mediated by inhibiting the SUMOylation process and increasing NF-κB p65 activity.

show abstract

“…COVID-19 is a global pandemic disease that has affected hundreds of millions of people worldwide. Most patients with COVID-19 may have mild to moderate symptoms such as cough and shortness of breath in the first week, and some patients may show extensive pneumonia, ultimate organ failure and diffuse intravascular coagulation in approximately three weeks ( Fenollar et al, 2021 ; Li et al, 2023 ). The main cause of death from COVID-19 is acute lung injury (ALI).…”

Section: Introductionmentioning

confidence: 99%