XRF analysis of micronutrients in endive grown on soils with sewage sludge

Moringa oleifera is a multipurpose plant used in Ghana and most parts of Africa. Its high mineral, protein, and vitamins content has enabled its use as a nutraceutical and panacea for various diseases. This study aimed at measuring the micro- and macroelements content of dried Moringa oleifera leaves using energy dispersive X-ray fluorescence spectroscopic (EDXRF) and assessing its toxicological effect in rats. Acute toxicity (5000 mg/kg) and a subacute toxicity studies of the leaf (40 mg/kg to 1000 mg/kg) extract were conducted in rats. Blood samples were assessed for biochemical and haematological parameters. Results showed significant levels of thirty-five (35) elements (14 macroelements and 21 microelements) in M. oleifera extract. There were no observed overt adverse reactions in the acute and subacute studies. Although there were observed elevations in liver enzymes ALT and ALP (P < 0.001) and lower creatinine levels in the extract treated groups, no adverse histopathological findings were found. Moringa oleifera dried leaf extract may, therefore, be reasonably safe for consumption. However, the consumption of Moringa oleifera leaves should not exceed a maximum of 70 grams per day to prevent cumulative toxicity of these essential elements over long periods.

show abstract

“…Copper deficiency, although rare, results in hypochromic anaemia [26]; possible side-effects may include liver damage and Wilson's syndrome.…”

Section: Discussionmentioning

confidence: 99%

Micro- and Macroelemental Composition and Safety Evaluation of the NutraceuticalMoringa oleiferaLeaves

Asiedu-Gyekye

Frimpong–Manso

Awortwe

et al. 2014

Journal of Toxicology

View full text Add to dashboard Cite

show abstract

“…25 However, the time between pelletization and analysis should be as short as possible in order to avoid deformation of the flat surfaces of the pellets, as pointed out previous work. 17,18,25 Considering the morphology of plant powder and the capacity to be compacted together, the manufacture of a pellet can hardly be done without addition of a binder. Following recommendations and details from previous work, 17,18,25,26 we prepared a powder pellet by mixing 2 g of plant powder with 0.2 g of pure wax (Hoechst Cridust wax) and homogenising the mixture by using an agate mortar.…”

Section: Sampling and Pretreatment For Wdxrf-edxrf Analysismentioning

confidence: 99%

“…17,18,25 Considering the morphology of plant powder and the capacity to be compacted together, the manufacture of a pellet can hardly be done without addition of a binder. Following recommendations and details from previous work, 17,18,25,26 we prepared a powder pellet by mixing 2 g of plant powder with 0.2 g of pure wax (Hoechst Cridust wax) and homogenising the mixture by using an agate mortar. These prepared samples were pressed at 20 t for 120 s to obtain a cylindrical pellet 40 mm in diameter.…”

Section: Sampling and Pretreatment For Wdxrf-edxrf Analysismentioning

confidence: 99%

Quantitative determination of essential and trace element content of medicinal plants and their infusions by XRF and ICP techniques

et al. 2005

View full text Add to dashboard Cite

Macro-and microelement contents of five medicinal plants (Taraxacum officinale Weber, Eucalyptus globulus Labill, Plantago lanceolata L., Matricaria chamomilla L. and Mentha piperita L.) and their infusions were evaluated by the combined use of x-ray fluorescence (WDXRF and EDXRF, bulk raw plants) and inductively coupled plasma (ICP-MS and ICP-AES, infusions) techniques. The analytical methods allow the determination of 17 elements (Na, Mg, Al, Si, P, S, K, Ca, Ti, Mn, Fe, Cu, Zn, As, Rb, Sr, and Pb) both in plants and in the infusions. The use of XRF techniques offer a good multielemental approach for the rapid quality control of bulk raw plant materials whereas ICP techniques are well suited for the analytical control of infusions in order to ascertain the nutritional role of medicinal plants and the daily dietary intake.

show abstract

“…For example, 30% of sludge is applied to agricultural land in Japan (Ito et al, 2000). In addition to N, sludges often contain micronutrients that could benefit crop growth (Abdou and El-Nennah, 1980;Perez-Murcia et al, 2006;Vazquez et al, 2003). However, metal loadings to soil from repeated sludge applications can cause reduced numbers of nitrogen fixing bacteria in soil (McGrath et al, 1988;McGrath et al, 1995) and result in a shortage of arbuscular mycorrhizal fungi (del Val et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Soil and Crop Micronutrient Concentrations as Modified by Wastewater Sludge

McGonigle

Evans

Wells³

2014

JEE

View full text Add to dashboard Cite

Soil extracts are used to provide a measure of plant nutrient bioavailability, but their predictive capacity is variable. In a greenhouse experiment, soil was amended with sewage sludge from textile manufacturing wastewater. We investigated the correspondence between maize (Zea mays L.) shoot uptake and concentrations in soil extractions for plant essential micronutrients concentrated in the sludge: B, Cu, Mn, and Zn. Hot water extract was used for B, whereas diethylene triamine pentaacetic acid (DTPA) extract was used for Cu, Mn, and Zn. Separate pots with NH 4 NO 3 amendment, but no sludge, allowed comparison of soil micronutrient extraction and plant uptake under conditions of elevated mineral N fertility similar to those with sludge amendment, but in the absence of sludge. Addition of sludge did not change soil extractable concentrations nor shoot concentrations of B and Zn. B was ISSN 2157-6092 2014 www.macrothink.org/jee 34 abundant in the sludge but likely remained strongly bound as organic complexes, whereas the quantity of Zn was not high enough to register difference. Shoot Mn was increased by both sludge amendment and an NH 4 NO 3 effect, likely from pH decrease associated with nitrification of the ammonium in the sludge and in the fertilizer. DTPA-extractable Mn increased in sludge-amended plots. DTPA-extractable Cu increased strongly following sludge amendment, but, in contrast to Mn, shoot Cu concentration was not changed. Thus, selectivity of roots to avoid Cu is not revealed by DTPA. The correspondence between extraction-based estimation of availability and the actual plant uptake varies among micronutrients due to effects of soil conditions and selectivity of roots. Journal of Environment and Ecology

show abstract

XRF analysis of micronutrients in endive grown on soils with sewage sludge

Cited by 15 publications

References 7 publications

Micro- and Macroelemental Composition and Safety Evaluation of the NutraceuticalMoringa oleiferaLeaves

Micro- and Macroelemental Composition and Safety Evaluation of the NutraceuticalMoringa oleiferaLeaves

Quantitative determination of essential and trace element content of medicinal plants and their infusions by XRF and ICP techniques

Soil and Crop Micronutrient Concentrations as Modified by Wastewater Sludge

Contact Info

Product

Resources

About