XRD, SEM-EDS, and FTIR studies ofin vitro growth of an apatite-like layer on sol-gel glasses

Abstract

A glass with a composition (in mole %) of: SiO2 (70), CaO (26), and P2O5 (4) was obtained using a sol-gel method. The in vitro bioactivity of the glass was assessed by determining the changes in surface morphology and composition after soaking in simulated body fluid (SBF) for periods of up to 14 days at 37 degrees C. X-ray diffraction, scanning electron microscopy, X-ray energy dispersive spectroscopy, and FTIR analyses of the glass surface after different soaking periods in SBF demonstrated the growth of an apatite-like layer on the glass surface. In the first stage, an amorphous calcium phosphate layer was formed; after 7 days this surface consisted of spheres, with diameters ranging between 2 and 15 microm, composed of needle-like apatite crystallites (250 x 100 nm) with a crystallinity similar to that of a biological apatite.

The effect of phosphate content on the bioactivity of soda-lime-phosphosilicate glasses

“…(7) show FTIR spectra of selected glasses from both series immersed in SBF for up to 21 days. For the glasses containing phosphate, in the 500 to 600 cm -1 region there is a broad feature corresponding to the P-O bending mode and also a Si-O-Si bending vibration in this region [13,[15][16][17]. Around 720 cm -1 in the glass spectra is the band due to Si-O bending vibrations [18,19].…”

“…This decrease in pH is advantageous for bioactivity as a pH of 7.2 is optimal in physiological fluid for apatite deposition [8]. corresponding to the (002) and (211) and reflections [12,13]. The region containing the (211) peak (30 to 35) also contains a number of other features, namely the (112), (300) and (202) reflections [12,13].…”

“…corresponding to the (002) and (211) and reflections [12,13]. The region containing the (211) peak (30 to 35) also contains a number of other features, namely the (112), (300) and (202) reflections [12,13]. However due to the noise and degree of peak overlap, these lines could not be used for crystallite size analysis.…”

The effect of serum proteins on apatite growth for 45S5 Bioglass and common sol-gel derived glass in SBF

“…Amorphous Ca/P compounds precipitate on the silica gel layer and crystallise into the needle-like HCA. Here, peaks at approximately 26 ∘ and 32 ∘ 2θ were found in the XRD patterns of the filtered BG particles in pSBF after 1 day of immersion and these peaks match the XRD pattern of hydroxylapatite (HA) [19]. Comparing the XRD patterns of BG ( Figure 1a) and TG ( Figure 1b) following immersion in pSBF, HA peaks did not appear for the TG glass until 7 day, compared to 1 day for BG, and the intensity and sharpness were less than for BG.…”

Understanding Biomineralization of Polycaprolactone Surfaces

“…For further in-depth technical details, please consult Jenkins et al 80 . An example of tracking the formation of HA through SBF immersion with XRD is demonstrated in the figure below from Vallet-Regí et al 81 . DCPD also has an established diffractogram that has previously been used as a means to illuminate changes in the phase composition of DCPD granules towards HA when in the presence of amino acids such as citrate 82 .…”