XPS analysis of chemical functions at the surface of Bacillus subtilis

Ahimou, François; Boonaert, Christophe J. P.; Adriaensen, Yasmine; Jacques, Philippe; Thonart, Philippe; Paquot, Michel; Rouxhet, Paul

doi:10.1016/j.jcis.2007.01.055

Cited by 127 publications

(97 citation statements)

References 41 publications

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“…The result is in accordance with the significant increase in easily oxidized carbon contents. This is considered to be probably associated with the oxidized matter formed on the surface of biochar, and also in agreement with founding of Qian and Chen [33]. Furthermore, DRIFTS and XPS analyses indicated that the surfaces of biochar exposed to higher temperatures underwent decarboxylation and hydroxylation to form alcohol/ether functional groups.…”

Section: Discussionsupporting

confidence: 87%

Effect of aging on surface chemistry of rice husk‐derived biochar

Huang

Zhou

et al. 2017

Env Prog and Sustain Energy

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Herein, we aimed to determine the physicochemical properties of biochar produced from rice husk at 350 and 5508C, to characterize changes in the biochar properties during aging and to identify the effect of aging time on these changes. To simulate different stages in the aging process, samples were incubated at a constant temperature in the dark and maintained at 60% water holding capacity for 100-300 d. The transformation and morphology of the biochar particles were monitored by diffuse-reflectance Fourier transform infrared spectroscopy (DRIFTS), scanning electron microscopy (SEM) coupled with energy X-ray dispersive spectroscopy, and X-ray photoelectron spectroscopy (XPS). During aging, a layer formed on the surface and the content of easily oxidized substances increased with increased aging time. In addition, SEM indicated that oxidation occurred close to the biochar surface rather than in the biochar particle interior. Furthermore, the DRIFTS and XPS results indicated that the biochar surface produced at 5508C underwent decarboxylation and hydroxylation, while for the 3508C biochar, the increase in surface oxygen content was associated with the presence of hydroxyl moieties.

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Section: Discussionsupporting

confidence: 87%

Effect of aging on surface chemistry of rice husk‐derived biochar

Huang

Zhou

et al. 2017

Env Prog and Sustain Energy

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“…The above could be attributed to the fact that the presence of carboxyl groups is not restricted to amides, as demonstrated by FTIR spectra. Our results are consistent with the findings of other authors [19,23].…”

Section: Xps Spectroscopysupporting

confidence: 94%

Assignment of functional groups in Gram-positive bacteria

Buszewski¹

2015

J Anal Bioanal Tech

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The chemical composition, molecular structure and physicochemical properties of five Gram-positive bacterial strain: Bacillus cereus, Bacillus subtilis, Sarcina lutea, Staphylococcus aureus, Micococcus luteus were investigated by Fourier transform infrared (FTIR), X-ray photoelectron spectroscopy (XPS), NMR spectroscopy and intact cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (IC MALDI TOF MS). An analysis of FTIR spectra as a function of pH revealed the three major types of cell wall functional groups -carboxyl group, amino group and phosphate group. An analysis of XPS spectra was determinate the major surface components of bacterial cell.13 C NMR and IC MALDI TOF MS spectra of six bacterial species were registered. Our findings indicate that chemical and structural differences in the cell composition of Gram-positive bacteria can be detected.The obtained results also demonstrate that the combination of FTIR, XPS and NMR spectroscopy with IC MALDI TOF MS technique yields useful information and complements other biochemical and physical methods of microbial cells characteristics.

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“…The binding energy of the spectrometer has been calibrated by referencing to the C1s peak at 284.8 eV. It is a common practice to use the carbon C 1s peak at 284.8 eV as reference for charge compensation as the C1s component is bound exclusively to carbon or hydrogen (Ahimou et al, 2007;Liu et al, 2014;Olivares et al, 2006;Yang et al, 2016). Peak fitting was performed with custom VBA program in Microsoft Excel using Voigt profiles together with a Shirley background function…”

Section: X-ray Photoelectron Spectroscopy (Xps)mentioning

confidence: 99%

“…This peak could be an artifact, or an unidentified C species. Currently, most studies (Ahimou et al, 2007;Liu et al, 2014;Yang et al, 2016) assigned the major C(1) peak as the lowest C peak with the binding energy at 284.8 eV. However, literature is available for C species with lower binding energy values.…”

Section: Xps Analysismentioning

confidence: 99%

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Surface Characterization of Cottonseed Meal Products by SEM, SEM-EDS, XRD and XPS Analysis

He¹,

Cheng²,

Olanya³

et al. 2017

JMSR

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The utilization of cottonseed meal products as valuable industrial materials needs to be exploited. We have recently produced water-washed cottonseed meal, total cottonseed protein, sequentially extracted water-and alkali-soluble proteins, and two residues after the total and sequential protein extractions at a pilot scale. In this work, the surface characteristics of the six cottonseed meal products were examined by scanning electron microscopy (SEM), scanning electron microscopy-energy dispersive spectrometer (SEM-EDS), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The results showed that the surface properties of the six products differed from those of a commercial soy protein flour examined comparatively in this work. The compact morphology and relative-high N composition were observed in all three protein products, with greater similarity between the total protein and alkali-soluble protein. The surfaces of the two residue products were more porous with polysaccharide features. Washed cottonseed meal possessed the surface features similar to those of the residues. In the meantime, the N-associated functional groups were under-represented in the surfaces of all samples, compared to their bulk composition. Information derived from this work increased the understanding of the surface functional properties of cottonseed meal products, which would benefit their practical utilization.

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XPS analysis of chemical functions at the surface of Bacillus subtilis

Cited by 127 publications

References 41 publications

Effect of aging on surface chemistry of rice husk‐derived biochar

Effect of aging on surface chemistry of rice husk‐derived biochar

Assignment of functional groups in Gram-positive bacteria

Surface Characterization of Cottonseed Meal Products by SEM, SEM-EDS, XRD and XPS Analysis

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