Assignment of functional groups in Gram-positive bacteria

Abstract

The chemical composition, molecular structure and physicochemical properties of five Gram-positive bacterial strain: Bacillus cereus, Bacillus subtilis, Sarcina lutea, Staphylococcus aureus, Micococcus luteus were investigated by Fourier transform infrared (FTIR), X-ray photoelectron spectroscopy (XPS), NMR spectroscopy and intact cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (IC MALDI TOF MS). An analysis of FTIR spectra as a function of pH revealed the three major types of cell wall functional groups -carboxyl group, amino group and phosphate group. An analysis of XPS spectra was determinate the major surface components of bacterial cell.13 C NMR and IC MALDI TOF MS spectra of six bacterial species were registered. Our findings indicate that chemical and structural differences in the cell composition of Gram-positive bacteria can be detected.The obtained results also demonstrate that the combination of FTIR, XPS and NMR spectroscopy with IC MALDI TOF MS technique yields useful information and complements other biochemical and physical methods of microbial cells characteristics.

Effects of Phosphorus in Growth Media on Biomineralization and Cell Surface Properties of Marine Cyanobacteria Synechococcus

“…PCC8806, vC-O-C of polysaccharides and v(CO) of the C-O-C glycosidic bridge were most sensitive to pH changes. The impact of carboxylic function groups was consistent with the results from the titration and FTIR observations previously described [31,32,34].…”

“…PCC8806 cell envelope were proteins, and their weight fraction tended to increase with P in the media (Figure 2, Table 2). Quantitative variations of the functional groups associated with carboxylic functions C-(O,N)/C (286.3 eV) and O = C, P = O or P-O-P (531.3 eV), and hydroxide (C-OH) or (C-O-C) (532.7 eV) were observed [31,32,34]. The discrepancy between these bands in the different cells was mostly noted in Syn.…”

“…For carbon (C1s), the most significant peak was found at the binding energy of~284.8 eV, which was linked to carbon and hydrogen, C-(C,H) in amino acids or lipids ( Table 2). The lowest value for this peak was found to be associated with cells cultured in~90 µM of P. The other C1s components were found at~286.3 eV, assigned to carbon singly bound to oxygen or nitrogen (C-(O,N)), including ether, alcohol, amine, and amide; at~287 eV, assigned to carbon forming a double bond with oxygen C = O or two single bonds O-C-O attributed to carbonyl, carboxyl groups, amide, acetyl, and hemiacetal; and at~288 eV, associated with carbon forming one double bond and one single bond with oxygen C = O-O, including the carboxyl group, and C = O-OR, including esters [31,32,34]. The other C1s components were found at~286.3,~287 eV, and~288 eV and were found to be higher for cells cultured in 90 µM of P, in particular the peak at 288 eV ( Table 2).…”

“…The oxygen peak (O1s) was deconvolved into three components: A peak at 531.1 eV corresponding to oxygen forming a double bond with carbon O = C and oxygen forming a double bond with phosphate P = O or oxygen forming a single bond with phosphate P-O-ring in the esters, amides, and phosphate group; a peak at 532.1 eV attributed to hydroxide, acetal, and hemiacetal; and a peak at 533.5 eV associated with oxygen forming a single bond with hydrogen (HO-C), including the hydroxyl group, or carbon (C-O-C), including acetals and hemiacetals [31,32,34]. We observed a decrease of O1s at 286.28 eV (O1s A) and an increase of O1s at 287.9 eV (O1s B) in the Syn.…”

Study of silver nanoparticles synthesized by acidophilic strain of Actinobacteria isolated from the of Picea sitchensis forest soil

“…(Buszewski et al . ). Deprotonated functional groups of antibiotics may bind of the released silver cations.…”