Xiaoaiping Induces Developmental Toxicity in Zebrafish Embryos Through Activation of ER Stress, Apoptosis and the Wnt Pathway

Li, Juanjuan; Zhang, Yun; Liu, Kechun; He, Qiuxia; Sun, Chen; Han, Jian; Han, Liwen; Tian, Qingping

doi:10.3389/fphar.2018.01250

Cited by 50 publications

(36 citation statements)

References 48 publications

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“…The rate of hatching, which normally occurs with zebrafish embryo from 48 to 96 hpf, is a critical indicator of the developmental state of these embryos [64,65]. At a concentration of 25 μg/L, AEO-7 eliminated hatching (Fig.…”

Section: Aeo-7 Did Not Affect the Hatching Rate (Hr)mentioning

confidence: 99%

AEO-7 surfactant is “super toxic” and induces severe cardiac, liver and locomotion damage in zebrafish embryos

et al. 2020

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Background Fatty alcohol polyoxyethylene ether-7 (AEO-7), a non-ionic surfactant, has recently been receiving extensive attention from the ocean pipeline industry for its ability to inhibit corrosion. However, the present lack of information concerning the potential environmental toxicity of AEO-7, especially towards aquatic organisms, is a major impediment to its wider application. Here, we assess potential adverse effects of AEO-7 on zebrafish embryos employing a variety of assays, including (i) a mortality/survival assay which allowed the median lethal concentration (LC50) to be calculated; (ii) a teratogenicity assay on the basis of which the no observed effect concentration (NOEC) was determined; and (iii) specific assays of cardiotoxicity, neurotoxicity (based on locomotion), hematopoietic toxicity (the level of hemoglobin as revealed by o-dianisidine staining) and hepatotoxicity (liver steatosis and yolk retention examined by staining with Oil Red O). Results AEO-7 caused mortality with a calculated LC50 of 15.35 μg/L, which, according to the U.S. Fish and Wildlife Service (USFWS) Acute Toxicity Rating scale, should be considered “super toxic”. Although at its NOEC (0.8 μg/L), there were no signs of significant teratogenicity, cardiotoxicity, or hemopoiesis toxicity, 3.2 µg/L AEO-7 exerted dramatic detrimental effects on organ development. Conclusion On the basis of these findings, we recommend that the industrial usage and environmental impact of AEO-7 be re-evaluated and strictly monitored by environmental and public health organizations.

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Section: Aeo-7 Did Not Affect the Hatching Rate (Hr)mentioning

confidence: 99%

AEO-7 surfactant is “super toxic” and induces severe cardiac, liver and locomotion damage in zebrafish embryos

et al. 2020

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“…However, they focused on two endpoints in their experiment: abnormal spastic behaviors (72 hpf) and alternations in the larval photomotor responses assay (120 hpf). Li et al [ 99 ] exposed zebrafish to Xiaoping at high doses, and many larvae opted to swim at a medium to a low rate, which led to decreased locomotion ability. Changes in larval behavior have been confirmed to be associated with damage to the central nervous system.…”

Section: Chemical Studies Of Embryotoxicitymentioning

confidence: 99%

Zebrafish as a Successful Animal Model for Screening Toxicity of Medicinal Plants

Chahardehi

Arsad

Lim

2020

Plants

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The zebrafish (Danio rerio) is used as an embryonic and larval model to perform in vitro experiments and developmental toxicity studies. Zebrafish may be used to determine the toxicity of samples in early screening assays, often in a high-throughput manner. The zebrafish embryotoxicity model is at the leading edge of toxicology research due to the short time required for analyses, transparency of embryos, short life cycle, high fertility, and genetic data similarity. Zebrafish toxicity studies range from assessing the toxicity of bioactive compounds or crude extracts from plants to determining the optimal process. Most of the studied extracts were polar, such as ethanol, methanol, and aqueous solutions, which were used to detect the toxicity and bioactivity. This review examines the latest research using zebrafish as a study model and highlights its power as a tool for detecting toxicity of medicinal plants and its effectiveness at enhancing the understanding of new drug generation. The goal of this review was to develop a link to ethnopharmacological zebrafish studies that can be used by other researchers to conduct future research.

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“…Hatching is a critical stage in the embryogenesis of Danio rerio for it aids in the evaluation of developmental delays and toxicity caused by different substances [93]. A normally developing Danio rerio typically hatches between 48 to 72 hpf [94].…”

Section: Hatchingmentioning

confidence: 99%

Embryotoxic and teratogenic effects of polyethylene microbeads found in facial wash products in Zebrafish (Danio rerio)using the Fish Embryo Acute Toxicity Test

Guzman

Chua

Sedano

2020

Preprint

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Use of polyethylene beads in facial cleansers has been continuously questioned by scientific communities for they adversely affect aquatic organisms once these beads find their way into their habitats. This study specifically aims to determine Danio rerio mortality rate using lethal endpoints and to evaluate sublethal teratogenic effects in Danio rerio due to polyethylene microbead exposure. Danio rerio, a model organism for ecotoxicology, was subjected to the Fish Embryo Acute Toxicity Test. Embryos were exposed to polyethylene microbead suspensions (PE-MBS) of varying concentrations (i.e., 20 μg/L, 200 μg/L, 2000 μg/L). They were also exposed to 5% ethanol (positive control), reconstituted water (negative control), 0.01% Tween 80 (emulsifier control), and 1% DMSO (solvent control). Toxicological endpoints (i.e., egg coagulation, lack of somite formation, non-detachment of tail, and lack of heartbeat) were observed every 24 hours until the 96th hour exposure. Hatching was observed from 48 hpf while teratogenicity was observed at 144 hpf. Significant differences between means and variances were observed for all treatment groups in relation to the negative control. For all groups, 0.01% Tween 80, 1% DMSO and 20 μg/L PE-MBS did not significantly differ with the negative control due to negligible concentration but 5% ethanol and higher concentrations of PE-MBS did. This indicated that high concentrations of PE-MBS exposure may induce early hatching, mortality, increased malformation, and increased heart rate. Tukey Kramer post hoc Test substantiated that PE-MBS toxicity is dose dependent since embryotoxicity and teratogenicity increases at higher concentrations. LC50 obtained using probit analysis based on experimental data was 2455.096 μg/L, and was higher than the concentrations used in this study. Further studies should be conducted to know more about the adverse effects of polyethylene microbeads to the biota.Author SummaryMargaret De Guzman, MSc, Patricia Chua, and Franceska Sedano have all equally contributed to this work in conceptualization, formal analysis, funding acquisition, and investigation. All authors have also equally headed project administration, procurement of resources and writing.

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Xiaoaiping Induces Developmental Toxicity in Zebrafish Embryos Through Activation of ER Stress, Apoptosis and the Wnt Pathway

Cited by 50 publications

References 48 publications

AEO-7 surfactant is “super toxic” and induces severe cardiac, liver and locomotion damage in zebrafish embryos

AEO-7 surfactant is “super toxic” and induces severe cardiac, liver and locomotion damage in zebrafish embryos

Zebrafish as a Successful Animal Model for Screening Toxicity of Medicinal Plants

Embryotoxic and teratogenic effects of polyethylene microbeads found in facial wash products in Zebrafish (Danio rerio)using the Fish Embryo Acute Toxicity Test

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