Xenopus laevis is a potential alternative model animal species to study reproductive toxicity of phytoestrogens

Lin, Cong; Qin, Zhan-Fen; Jing, Xiang-Ning; Yang, Lei; Zhou, Jingming; Xu, Xing

doi:10.1016/j.aquatox.2005.12.014

Cited by 13 publications

(17 citation statements)

References 47 publications

(45 reference statements)

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“…The present results confirm an earlier report (Skibola and Smith, 2000) which showed that these compounds cross the placenta and place the unborn fetus at great risk. It has shown that exposure of X. laevis to quercetin was found to cause abnormal testes, including necrosis, apoptosis of spermatogonia, delay in the development of seminiferous tubules (Cong et al, 2006). The treatment with fenugreek was also found to induce significant changes in the chromosomal aberrations after sub-chronic treatment.…”

Section: Discussionmentioning

confidence: 82%

“…The mode of action of fenugreek on the observed reproductive, cytological and biochemical changes is not known. Nevertheless, it may be related with the generation of oxygen radicals by some of the toxic constituents of fenugreek (Wu et al, 1997;Kumar and Muralidhara 1999;Skibola and Smith, 2000;Cong et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…Quercetin has been shown to cause abnormal testes with necrosis, apoptosis of spermatogonia, delay in the development of seminiferous tubes in Xenopus laevis (Cong et al, 2006). Quinones and trigonelline have been found to be cytotoxic and are mutagenic in bacteria (Wu et al, 1997), act as pro-oxidants (Skibola and Smith, 2000), depletion of serum triiodothyronine (T3) concentration and T3/T4 ratio, but increases thyroxine (T4) levels both in mice and rats (Panda et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

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Reproductive, cytological and biochemical toxicity of fenugreek in male Swiss albino mice

Al-Yahya¹

2013

Afr. J. Pharm. Pharmacol.

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Fenugreek was evaluated for its effects on reproductive, cytological and biochemical toxicity in mice.On the basis of evaluated maximum tolerated dose (MTD; 9.77 g/kg), the present doses selected for sub-chronic (90 days) treatment were 153, 305 and 610 mg/kg/day by oral gavage corresponding to 1/64, 1/32 and 1/16, of MTD, respectively. Total sperm count, motility and spermatozoa morphology was screened. Cytological changes in testicular chromosomes and pregnancy rate in untreated females after mating with treated males were estimated. In testicular cells, total proteins, nucleic acids, malondialdehyde (MDA) and non-protein sulfhydryl (NP-SH) and serum hormonal levels were estimated. The fenugreek treatment particularly with higher dose caused significant changes in the percent motility, sperm count, spermatozoa morphology, chromosomal aberrations, rate of pregnancy and preimplantation loss. Male fertility was decreased in higher treated doses. In testicular cells, nucleic acids and NP-SH were depleted while MDA levels increased. In conclusion, fenugreek administration at higher dose induced toxicity including teratogenic, foetotoxic, reproductive changes and the abnormal shapes of the sperms. In view of the observed oxidant potentials, present study paves a path to further investigate its clinical effects on reproductive system.

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Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Reproductive, cytological and biochemical toxicity of fenugreek in male Swiss albino mice

Al-Yahya¹

2013

Afr. J. Pharm. Pharmacol.

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“…Additionally, rats fed a genistein supplemented diet during development had decreased sperm counts as adults [62]. Adverse effects of phytoestrogens on testes have also been found in fish [63] and frogs [64]. Few studies have examined the effects of phytoestrogens on avian testes, but Opalka et al (2006) found that various phytoestrogens inhibited testosterone production in cultured Bilgoraj gander Leydig cells [65].…”

Section: Discussionmentioning

confidence: 99%

Dietary red clover (Trifolium pratense) induces oviduct growth and decreases ovary and testes growth in Japanese quail chicks

Rochester

Klasing

Stevenson

et al. 2009

Reproductive Toxicology

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To determine whether drought-stress alters phytoestrogens in red clover and whether red clover in the diet influences sexual development in Japanese quail, we fed chicks diets containing irrigated or non-irrigated clover. Irrigation altered phytoestrogenic activity of red clover (determined using an in vitro bioassay), with extracts of irrigated clover diet containing more estrogenic activity than extracts of non-irrigated clover diet. Chick growth was negatively correlated with the amount of irrigated or non-irrigated clover in the diet. Dietary red clover also depressed both absolute and relative gonad weights; however, relative oviduct weight was increased by the irrigated diet. Diets did not affect serum vitellogenin. These results reveal a negative influence of drought-stress on phytoestrogenic potency of clover, and that red clover in the diet can inhibit avian growth and development independent of irrigation state. Thus, phytoestrogens may affect reproductive development in wild birds, and environmental stressors may influence levels of phytoestrogens in the field.

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“…Although the uterotrophic assay is one of the most extensively used in vivo assays for estrogenicity, it may not be suitable for chemicals such as selective estrogen-receptor modulators (SERMs) or those predictive for dose (Diel et al, 2002;Owens and Ashby, 2002). In a Xenopus assay, embryos/eggs were exposed to test chemicals, quercetin, or control solvent, and, after certain periods of time, frogs were subjected to morphological and histological examinations (Cong et al, 2006). The results of animal tests are often reevaluated by other assays to confirm the effects or to find the differences in species, principles or materials among the assays; for example, the rainbow trout assay was reevaluated by reverse-transcription polymerase chain-reaction (RT-PCR), DNA microarray assay and Western blotting (Tilton et al, 2006), rodent uterotrophic assay by ligand-binding assay (Owens and Ashby, 2002) and medaka assay by signaling pathway analyses using human breast cancer cells (Li et al, 2016).…”

Section: Animal Testmentioning

confidence: 99%

Estrogenic Potentials of Traditional Chinese Medicine

Kiyama

2017

Am. J. Chin. Med.

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Estrogen, a steroid hormone, is associated with several human activities, including environmental, industrial, agricultural, pharmaceutical and medical fields. In this review paper, estrogenic activity associated with traditional Chinese medicines (TCMs) is discussed first by focusing on the assays needed to detect estrogenic activity (animal test, cell assay, ligand-binding assay, protein assay, reporter-gene assay, transcription assay and yeast two-hybrid assay), and then, their sources, the nature of activities (estrogenic or antiestrogenic, or other types), and pathways/functions, along with the assay used to detect the activity, which is followed by a summary of effective chemicals found in or associated with TCM. Applications of estrogens in TCM are then discussed by a comprehensive search of the literature, which include basic study/pathway analysis, cell functions, diseases/symptoms and medicine/supplements. Discrepancies and conflicting cases about estrogenicity of TCM among assays or between TCM and their effective chemicals, are focused on to enlarge estrogenic potentials of TCM by referring to omic knowledge such as transcriptome, proteome, glycome, chemome, cellome, ligandome, interactome and effectome.

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Xenopus laevis is a potential alternative model animal species to study reproductive toxicity of phytoestrogens

Cited by 13 publications

References 47 publications

Reproductive, cytological and biochemical toxicity of fenugreek in male Swiss albino mice

Reproductive, cytological and biochemical toxicity of fenugreek in male Swiss albino mice

Dietary red clover (Trifolium pratense) induces oviduct growth and decreases ovary and testes growth in Japanese quail chicks

Estrogenic Potentials of Traditional Chinese Medicine

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